Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids
<p>We examined the in vivo behavior of liver natural killer T cells (NKT cells) by intravital fluorescence microscopic imaging of mice in which a green fluorescent protein cDNA was used to replace the gene encoding the chemokine receptor CXCR6. NKT cells, which account for most CXCR6<sup>...
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doaj-4827ff8ffc334a9193a87518195f3cfb2021-07-02T03:51:09ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-01-0134e11310.1371/journal.pbio.0030113.20050521Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver SinusoidsGeissmann FredericCameron Thomas OSidobre StephaneManlongat NatashaKronenberg MitchellBriskin Michael JDustin Michael LLittman Dan RLittman Dan R<p>We examined the in vivo behavior of liver natural killer T cells (NKT cells) by intravital fluorescence microscopic imaging of mice in which a green fluorescent protein cDNA was used to replace the gene encoding the chemokine receptor CXCR6. NKT cells, which account for most CXCR6<sup>+</sup> cells in liver, were found to crawl within hepatic sinusoids at 10-20 µm/min and to stop upon T cell antigen receptor activation. CXCR6-deficient mice exhibited a selective and severe reduction of CD1d-reactive NKT cells in the liver and decreased susceptibility to T-cell-dependent hepatitis. CXCL16, the cell surface ligand for CXCR6, is expressed on sinusoidal endothelial cells, and CXCR6 deficiency resulted in reduced survival, but not in altered speed or pattern of patrolling of NKT cells. Thus, NKT cells patrol liver sinusoids to provide intravascular immune surveillance, and CXCR6 contributes to liver-based immune responses by regulating their abundance.</p> http://dx.doi.org/10.1371/journal.pbio.0030113Cell BiologyImmunology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Geissmann Frederic Cameron Thomas O Sidobre Stephane Manlongat Natasha Kronenberg Mitchell Briskin Michael J Dustin Michael L Littman Dan R Littman Dan R |
spellingShingle |
Geissmann Frederic Cameron Thomas O Sidobre Stephane Manlongat Natasha Kronenberg Mitchell Briskin Michael J Dustin Michael L Littman Dan R Littman Dan R Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids PLoS Biology Cell Biology Immunology |
author_facet |
Geissmann Frederic Cameron Thomas O Sidobre Stephane Manlongat Natasha Kronenberg Mitchell Briskin Michael J Dustin Michael L Littman Dan R Littman Dan R |
author_sort |
Geissmann Frederic |
title |
Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids |
title_short |
Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids |
title_full |
Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids |
title_fullStr |
Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids |
title_full_unstemmed |
Intravascular Immune Surveillance by CXCR6<sup>+</sup> NKT Cells Patrolling Liver Sinusoids |
title_sort |
intravascular immune surveillance by cxcr6<sup>+</sup> nkt cells patrolling liver sinusoids |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2005-01-01 |
description |
<p>We examined the in vivo behavior of liver natural killer T cells (NKT cells) by intravital fluorescence microscopic imaging of mice in which a green fluorescent protein cDNA was used to replace the gene encoding the chemokine receptor CXCR6. NKT cells, which account for most CXCR6<sup>+</sup> cells in liver, were found to crawl within hepatic sinusoids at 10-20 µm/min and to stop upon T cell antigen receptor activation. CXCR6-deficient mice exhibited a selective and severe reduction of CD1d-reactive NKT cells in the liver and decreased susceptibility to T-cell-dependent hepatitis. CXCL16, the cell surface ligand for CXCR6, is expressed on sinusoidal endothelial cells, and CXCR6 deficiency resulted in reduced survival, but not in altered speed or pattern of patrolling of NKT cells. Thus, NKT cells patrol liver sinusoids to provide intravascular immune surveillance, and CXCR6 contributes to liver-based immune responses by regulating their abundance.</p> |
topic |
Cell Biology Immunology |
url |
http://dx.doi.org/10.1371/journal.pbio.0030113 |
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