Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness
To adapt to changing environmental niches, bacteria require taxis, a movement toward or away from a stimulus (ligand). Chemotaxis has been studied in some members of the Soft Rot Pectobacteriaceae (SRP), particularly members of the genus Dickeya. On the contrary, there are fewer studies on this topi...
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doaj-4845f5b5334e4b4fbb55b115c91a804c2021-04-22T06:45:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.650894650894Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological FitnessCollins Kipngetich Tanui0Divine Yutefar Shyntum1Precious K. Sedibane2Precious K. Sedibane3Daniel Bellieny-Rabelo4Lucy N. Moleleki5Lucy N. Moleleki6Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South AfricaDepartment of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South AfricaDepartment of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South AfricaForestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South AfricaDepartment of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South AfricaDepartment of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South AfricaForestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South AfricaTo adapt to changing environmental niches, bacteria require taxis, a movement toward or away from a stimulus (ligand). Chemotaxis has been studied in some members of the Soft Rot Pectobacteriaceae (SRP), particularly members of the genus Dickeya. On the contrary, there are fewer studies on this topic for the other genus in the SRP group, namely Pectobacterium. This study evaluated chemotactic responses in Pectobacterium brasiliense (Pb 1692) to various ligands. A total of 34 methyl-accepting chemotactic proteins (MCPs) were identified in the Pb 1692 genome and the domain architectures of these MCPs were determined. Four Pb 1692 MCPs previously shown to be differentially expressed during potato tuber infection were selected for further functional characterization. Toward this end, Pb 1692 mutant strains each lacking either AED-0001492, AED-0003671, AED-0000304, or AED-0000744 were generated. Two of these mutants (AED-0001492 and AED-0003671), were attenuated in their ability to grow and respond to citrate and are thus referred to as MCPcit2 and MCPcit1, respectively, while the other two, AED-0000304 (MCPxyl) and AED-0000744 (MCPasp), were affected in their ability to respond to xylose and aspartate, respectively. Trans-complementation of the mutant strains restored swimming motility in the presence of respective ligands. The four MCP mutants were not affected in virulence but were significantly attenuated in their ability to attach to potato leaves suggesting that ecological fitness is an important contribution of these MCPs toward Pb 1692 biology.https://www.frontiersin.org/articles/10.3389/fpls.2021.650894/fullchemotaxissoft rotblackleg diseasePectobacterium brasilienseecological fitnessvirulence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Collins Kipngetich Tanui Divine Yutefar Shyntum Precious K. Sedibane Precious K. Sedibane Daniel Bellieny-Rabelo Lucy N. Moleleki Lucy N. Moleleki |
spellingShingle |
Collins Kipngetich Tanui Divine Yutefar Shyntum Precious K. Sedibane Precious K. Sedibane Daniel Bellieny-Rabelo Lucy N. Moleleki Lucy N. Moleleki Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness Frontiers in Plant Science chemotaxis soft rot blackleg disease Pectobacterium brasiliense ecological fitness virulence |
author_facet |
Collins Kipngetich Tanui Divine Yutefar Shyntum Precious K. Sedibane Precious K. Sedibane Daniel Bellieny-Rabelo Lucy N. Moleleki Lucy N. Moleleki |
author_sort |
Collins Kipngetich Tanui |
title |
Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness |
title_short |
Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness |
title_full |
Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness |
title_fullStr |
Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness |
title_full_unstemmed |
Pectobacterium brasiliense 1692 Chemotactic Responses and the Role of Methyl-Accepting Chemotactic Proteins in Ecological Fitness |
title_sort |
pectobacterium brasiliense 1692 chemotactic responses and the role of methyl-accepting chemotactic proteins in ecological fitness |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-04-01 |
description |
To adapt to changing environmental niches, bacteria require taxis, a movement toward or away from a stimulus (ligand). Chemotaxis has been studied in some members of the Soft Rot Pectobacteriaceae (SRP), particularly members of the genus Dickeya. On the contrary, there are fewer studies on this topic for the other genus in the SRP group, namely Pectobacterium. This study evaluated chemotactic responses in Pectobacterium brasiliense (Pb 1692) to various ligands. A total of 34 methyl-accepting chemotactic proteins (MCPs) were identified in the Pb 1692 genome and the domain architectures of these MCPs were determined. Four Pb 1692 MCPs previously shown to be differentially expressed during potato tuber infection were selected for further functional characterization. Toward this end, Pb 1692 mutant strains each lacking either AED-0001492, AED-0003671, AED-0000304, or AED-0000744 were generated. Two of these mutants (AED-0001492 and AED-0003671), were attenuated in their ability to grow and respond to citrate and are thus referred to as MCPcit2 and MCPcit1, respectively, while the other two, AED-0000304 (MCPxyl) and AED-0000744 (MCPasp), were affected in their ability to respond to xylose and aspartate, respectively. Trans-complementation of the mutant strains restored swimming motility in the presence of respective ligands. The four MCP mutants were not affected in virulence but were significantly attenuated in their ability to attach to potato leaves suggesting that ecological fitness is an important contribution of these MCPs toward Pb 1692 biology. |
topic |
chemotaxis soft rot blackleg disease Pectobacterium brasiliense ecological fitness virulence |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.650894/full |
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