Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress
Abstract Background Soil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated a...
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doaj-4846b7bd89f24653bc91bb4b4d68e02b2021-04-25T11:49:56ZengBMCJournal of Cotton Research2523-32542021-04-014111310.1186/s42397-021-00085-5Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stressLin CHEN0Heng SUN1Jie KONG2Haijiang XU3Xiyan YANG4National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), College of Plant Science and Technology, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), College of Plant Science and Technology, Huazhong Agricultural UniversityInstitute of Economic Crops, Xinjiang Academy of Agricultural SciencesInstitute of Economic Crops, Xinjiang Academy of Agricultural SciencesNational Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), College of Plant Science and Technology, Huazhong Agricultural UniversityAbstract Background Soil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis. Results Through transcriptome analysis in cotton leaves under salt stress for 0 h (T0), 3 h (T3) and 12 h (T12), we identified 8 436, 11 628 and 6 311 differentially expressed genes (DEGs) in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. A total of 459 differentially expressed proteins (DEPs) were identified by proteomic analysis, of which 273, 99 and 260 DEPs were identified in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. Metabolic pathways, biosynthesis of secondary metabolites, photosynthesis and plant hormone signal transduction were enriched among the identified DEGs or DEPs. Detail analysis of the DEGs or DEPs revealed that complex signaling pathways, such as abscisic acid (ABA) and jasmonic acid (JA) signaling, calcium signaling, mitogen-activated protein kinase (MAPK) signaling cascade, transcription factors, activation of antioxidant and ion transporters, were participated in regulating salt response in cotton. Conclusions Our research not only contributed to understand the mechanism of cotton response to salt stress, but also identified nine candidate genes, which might be useful for molecular breeding to improve salt-tolerance in cotton.https://doi.org/10.1186/s42397-021-00085-5CottonProteomeSalt stressTranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin CHEN Heng SUN Jie KONG Haijiang XU Xiyan YANG |
spellingShingle |
Lin CHEN Heng SUN Jie KONG Haijiang XU Xiyan YANG Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress Journal of Cotton Research Cotton Proteome Salt stress Transcriptome |
author_facet |
Lin CHEN Heng SUN Jie KONG Haijiang XU Xiyan YANG |
author_sort |
Lin CHEN |
title |
Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress |
title_short |
Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress |
title_full |
Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress |
title_fullStr |
Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress |
title_full_unstemmed |
Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress |
title_sort |
integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress |
publisher |
BMC |
series |
Journal of Cotton Research |
issn |
2523-3254 |
publishDate |
2021-04-01 |
description |
Abstract Background Soil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis. Results Through transcriptome analysis in cotton leaves under salt stress for 0 h (T0), 3 h (T3) and 12 h (T12), we identified 8 436, 11 628 and 6 311 differentially expressed genes (DEGs) in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. A total of 459 differentially expressed proteins (DEPs) were identified by proteomic analysis, of which 273, 99 and 260 DEPs were identified in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. Metabolic pathways, biosynthesis of secondary metabolites, photosynthesis and plant hormone signal transduction were enriched among the identified DEGs or DEPs. Detail analysis of the DEGs or DEPs revealed that complex signaling pathways, such as abscisic acid (ABA) and jasmonic acid (JA) signaling, calcium signaling, mitogen-activated protein kinase (MAPK) signaling cascade, transcription factors, activation of antioxidant and ion transporters, were participated in regulating salt response in cotton. Conclusions Our research not only contributed to understand the mechanism of cotton response to salt stress, but also identified nine candidate genes, which might be useful for molecular breeding to improve salt-tolerance in cotton. |
topic |
Cotton Proteome Salt stress Transcriptome |
url |
https://doi.org/10.1186/s42397-021-00085-5 |
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