Polycromatophilic cell count in differentiation of non regenerative anemia from regenerative anemia in dogs

• Main Authors: Nina Cunha Medeiros, Rosangela Locatelli Dittrich
• Format: Article
• Language: English
• Published: Universidade Estadual de Londrina 2014-10-01
• Series: Semina: Ciências Agrárias
• Subjects: Bone marrow response; Anemia; Dogs; Polychromatophilic Cell; Reticulocytes
• Online Access: http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/16029

The method of quantitation of polychromatophilic cell is routinely used in veterinary clinical laboratories to identify the bone marrow response. However, there is no standardization of this approach over the absolute reticulocyte count. The present study evaluated blood samples from 135 anemic dogs and 21 healthy dogs. CBC and quantification of reticulocytes and polychromatophilic cell were performed. The sensitivity, specificity, accuracy and Kappa of the polychromatophilic cell count were, respectively, 86.11%, 87.5%, 87.12% and 71%. The Pearson correlation coefficient (r) was 0.8125 and the coefficient of determination equation (R2) was 0.6603, between the number of polychromatophilic cell and reticulocytes. The identification of two polychromatophilic cells per field could allow a differentiation between regenerative and nonregenerative anemia, but it was not possible to associate it to the absolute reticulocyte count and classify the intensity of regeneration of anemia.