G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla
With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possess...
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doaj-4886badfafe248f2895e345ab05712702020-11-25T02:28:45ZengMDPI AGSensors1424-82202013-01-011311064107510.3390/s130101064G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophyllaJuan HuWensheng PangZhenzhu ZhengJing HanWith an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2− to blue-green ABTS•− by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2−-H2O2 system. The “turn-off” assay allows the detection of T-DNA from 1.0 × 10−9 to 3.0 × 10−7 mol·L−1 (R2 = 0.9906), with a low detection limit of 3.1 × 10−10 mol·L−1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines.http://www.mdpi.com/1424-8220/13/1/1064G-quadruplex DNAzymemolecular beaconcolorimetricPseudostellaria heterophylla |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Hu Wensheng Pang Zhenzhu Zheng Jing Han |
spellingShingle |
Juan Hu Wensheng Pang Zhenzhu Zheng Jing Han G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla Sensors G-quadruplex DNAzyme molecular beacon colorimetric Pseudostellaria heterophylla |
author_facet |
Juan Hu Wensheng Pang Zhenzhu Zheng Jing Han |
author_sort |
Juan Hu |
title |
G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
title_short |
G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
title_full |
G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
title_fullStr |
G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
title_full_unstemmed |
G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
title_sort |
g-quadruplex dnazyme molecular beacon for amplified colorimetric biosensing of pseudostellaria heterophylla |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2013-01-01 |
description |
With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2− to blue-green ABTS•− by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2−-H2O2 system. The “turn-off” assay allows the detection of T-DNA from 1.0 × 10−9 to 3.0 × 10−7 mol·L−1 (R2 = 0.9906), with a low detection limit of 3.1 × 10−10 mol·L−1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines. |
topic |
G-quadruplex DNAzyme molecular beacon colorimetric Pseudostellaria heterophylla |
url |
http://www.mdpi.com/1424-8220/13/1/1064 |
work_keys_str_mv |
AT juanhu gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla AT wenshengpang gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla AT zhenzhuzheng gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla AT jinghan gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla |
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