Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney
Considering the key role of renal dopamine in tubular sodium handling, we hypothesized that c-type natriuretic peptide (CNP) and Ang-(1-7) may regulate renal dopamine availability in tubular cells, contributing to Na+, K+-ATPase inhibition. Present results show that CNP did not affect either 3H-dopa...
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doaj-48970334847541f891ffd0d982de99882020-11-25T00:53:40ZengHindawi LimitedScientifica2090-908X2016-01-01201610.1155/2016/63023766302376Regulation of Dopamine Uptake by Vasoactive Peptides in the KidneyN. L. Rukavina Mikusic0N. M. Kouyoumdzian1E. Rouvier2M. M. Gironacci3J. E. Toblli4B. E. Fernández5M. R. Choi6Instituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaCátedras de Química Biológica, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaConsidering the key role of renal dopamine in tubular sodium handling, we hypothesized that c-type natriuretic peptide (CNP) and Ang-(1-7) may regulate renal dopamine availability in tubular cells, contributing to Na+, K+-ATPase inhibition. Present results show that CNP did not affect either 3H-dopamine uptake in renal tissue or Na+, K+-ATPase activity; meanwhile, Ang-(1-7) was able to increase 3H-dopamine uptake and decreased Na+, K+-ATPase activity in renal cortex. Ang-(1-7) and dopamine together decreased further Na+, K+-ATPase activity showing an additive effect on the sodium pump. In addition, hydrocortisone reversed Ang-(1-7)-dopamine overinhibition on the enzyme, suggesting that this inhibition is closely related to Ang-(1-7) stimulation on renal dopamine uptake. Both anantin and cANP (4-23-amide) did not modify CNP effects on 3H-dopamine uptake by tubular cells. The Mas receptor antagonist, A-779, blocked the increase elicited by Ang-(1-7) on 3H-dopamine uptake. The stimulatory uptake induced by Ang-(1-7) was even more pronounced in the presence of losartan, suggesting an inhibitory effect of Ang-(1-7) on AT1 receptors on 3H-dopamine uptake. By increasing dopamine bioavailability in tubular cells, Ang-(1-7) enhances Na+, K+-ATPase activity inhibition, contributing to its natriuretic and diuretic effects.http://dx.doi.org/10.1155/2016/6302376 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N. L. Rukavina Mikusic N. M. Kouyoumdzian E. Rouvier M. M. Gironacci J. E. Toblli B. E. Fernández M. R. Choi |
spellingShingle |
N. L. Rukavina Mikusic N. M. Kouyoumdzian E. Rouvier M. M. Gironacci J. E. Toblli B. E. Fernández M. R. Choi Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney Scientifica |
author_facet |
N. L. Rukavina Mikusic N. M. Kouyoumdzian E. Rouvier M. M. Gironacci J. E. Toblli B. E. Fernández M. R. Choi |
author_sort |
N. L. Rukavina Mikusic |
title |
Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
title_short |
Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
title_full |
Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
title_fullStr |
Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
title_full_unstemmed |
Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
title_sort |
regulation of dopamine uptake by vasoactive peptides in the kidney |
publisher |
Hindawi Limited |
series |
Scientifica |
issn |
2090-908X |
publishDate |
2016-01-01 |
description |
Considering the key role of renal dopamine in tubular sodium handling, we hypothesized that c-type natriuretic peptide (CNP) and Ang-(1-7) may regulate renal dopamine availability in tubular cells, contributing to Na+, K+-ATPase inhibition. Present results show that CNP did not affect either 3H-dopamine uptake in renal tissue or Na+, K+-ATPase activity; meanwhile, Ang-(1-7) was able to increase 3H-dopamine uptake and decreased Na+, K+-ATPase activity in renal cortex. Ang-(1-7) and dopamine together decreased further Na+, K+-ATPase activity showing an additive effect on the sodium pump. In addition, hydrocortisone reversed Ang-(1-7)-dopamine overinhibition on the enzyme, suggesting that this inhibition is closely related to Ang-(1-7) stimulation on renal dopamine uptake. Both anantin and cANP (4-23-amide) did not modify CNP effects on 3H-dopamine uptake by tubular cells. The Mas receptor antagonist, A-779, blocked the increase elicited by Ang-(1-7) on 3H-dopamine uptake. The stimulatory uptake induced by Ang-(1-7) was even more pronounced in the presence of losartan, suggesting an inhibitory effect of Ang-(1-7) on AT1 receptors on 3H-dopamine uptake. By increasing dopamine bioavailability in tubular cells, Ang-(1-7) enhances Na+, K+-ATPase activity inhibition, contributing to its natriuretic and diuretic effects. |
url |
http://dx.doi.org/10.1155/2016/6302376 |
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