Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study
Background: Benzo(a)pyrene (BaP) as an environmental pollutant is ubiquitous in the environment and it has destructive effects on human health. So far, various studies have demonstrated that BaP can cause adverse effects on the female reproductive system, but the existing information is limited abou...
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Shahid Sadoughi University of Medical Sciences
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doaj-489df1e7e29e437ea899e0b2ffe366b02020-11-25T02:18:07ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722018-12-011612745756Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental studyZeinab Mardanshahi0Abbasali Karimpour Malekshah1Fereshteh Talebpour Amiri2Reza Valadan3 Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Department of Immunology, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran Background: Benzo(a)pyrene (BaP) as an environmental pollutant is ubiquitous in the environment and it has destructive effects on human health. So far, various studies have demonstrated that BaP can cause adverse effects on the female reproductive system, but the existing information is limited about the effects of BaP on the endometrial receptivity and embryo implantation. Objective: The aim of this study was to investigate the effects of BaP on the endometrial receptivity and implantation in mice. Materials and Methods: In this experimental study, 40 pregnant BALB/c mice were divided into 5 groups (n = 8/each) as follows: experimental groups received the doses of 100 μg/kg, 200 μg/kg, and 500 μg/kg BaP dissolved in corn oil, the control group received normal saline and sham group received corn oil. Pregnant mice administered these solutions from Day 1 to Day 5 of gestation by gavage. On Day 6, the mice were sacrificed. Then their embryos were counted and the hormonal, histomorphological and molecular analyses were performed on themocusa of uterine tube. Results: The data revealed that BaP reduces estrogen and progesterone levels, decreases the number of implantation site, endometrium thickness, uterine lumen diameter, stromal cells and endometrial glands, and blood vessels in the endometrium. However, the expression of Activin receptor-like kinase 5 and E cadherin genes was not changed by BaP with different doses. Conclusion: The finding of this study showed that BaP can change estrogen and progesterone levels, and endometrial morphology leads to impairing the endometrial receptivity and decreasing the number of implantation site.http://journals.ssu.ac.ir/ijrmnew/article-1-1330-en.htmlbenzo(a)pyreneembryo implantationestrogenprogesteronealk5e-cadherin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zeinab Mardanshahi Abbasali Karimpour Malekshah Fereshteh Talebpour Amiri Reza Valadan |
spellingShingle |
Zeinab Mardanshahi Abbasali Karimpour Malekshah Fereshteh Talebpour Amiri Reza Valadan Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study International Journal of Reproductive BioMedicine benzo(a)pyrene embryo implantation estrogen progesterone alk5 e-cadherin |
author_facet |
Zeinab Mardanshahi Abbasali Karimpour Malekshah Fereshteh Talebpour Amiri Reza Valadan |
author_sort |
Zeinab Mardanshahi |
title |
Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study |
title_short |
Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study |
title_full |
Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study |
title_fullStr |
Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study |
title_full_unstemmed |
Effects of Benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: An experimental study |
title_sort |
effects of benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: an experimental study |
publisher |
Shahid Sadoughi University of Medical Sciences |
series |
International Journal of Reproductive BioMedicine |
issn |
2476-4108 2476-3772 |
publishDate |
2018-12-01 |
description |
Background: Benzo(a)pyrene (BaP) as an environmental pollutant is ubiquitous in the environment and it has destructive effects on human health. So far, various studies have demonstrated that BaP can cause adverse effects on the female reproductive system, but the existing information is limited about the effects of BaP on the endometrial receptivity and embryo implantation.
Objective: The aim of this study was to investigate the effects of BaP on the endometrial receptivity and implantation in mice.
Materials and Methods: In this experimental study, 40 pregnant BALB/c mice were divided into 5 groups (n = 8/each) as follows: experimental groups received the doses of 100 μg/kg, 200 μg/kg, and 500 μg/kg BaP dissolved in corn oil, the control group received normal saline and sham group received corn oil. Pregnant mice administered these solutions from Day 1 to Day 5 of gestation by gavage. On Day 6, the mice were sacrificed. Then their embryos were counted and the hormonal, histomorphological and molecular analyses were performed on themocusa of uterine tube.
Results: The data revealed that BaP reduces estrogen and progesterone levels, decreases the number of implantation site, endometrium thickness, uterine lumen diameter, stromal cells and endometrial glands, and blood vessels in the endometrium.
However, the expression of Activin receptor-like kinase 5 and E cadherin genes was not changed by BaP with different doses.
Conclusion: The finding of this study showed that BaP can change estrogen and progesterone levels, and endometrial morphology leads to impairing the endometrial receptivity and decreasing the number of implantation site. |
topic |
benzo(a)pyrene embryo implantation estrogen progesterone alk5 e-cadherin |
url |
http://journals.ssu.ac.ir/ijrmnew/article-1-1330-en.html |
work_keys_str_mv |
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