The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia
Blastocystis spp. has become one of the protozoans arousing the greatest scientific interest because of the controversy surrounding its biology; it is currently considered one of the most prevalent organisms in humans and animals worldwide. Such prevalence increases, especially in tropical countries...
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doaj-48a36489591543e1b784892543d0af6d2020-11-25T02:20:54ZengElsevierParasite Epidemiology and Control2405-67312020-05-019The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, ColombiaCaterine Potes-Morales0Laura A. Osorio-Delgado1Julio César Carranza2Gustavo Adolfo Vallejo3Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Barrio Santa Helena, Ibagué, ColombiaLaboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Barrio Santa Helena, Ibagué, ColombiaLaboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Barrio Santa Helena, Ibagué, ColombiaCorresponding author at: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Ibagué, Colombia.; Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Barrio Santa Helena, Ibagué, ColombiaBlastocystis spp. has become one of the protozoans arousing the greatest scientific interest because of the controversy surrounding its biology; it is currently considered one of the most prevalent organisms in humans and animals worldwide. Such prevalence increases, especially in tropical countries where infection rates are high, highlighting the need to conduct studies focused on understanding this protozoan's biology. Interestingly, molecular tools are emerging as the best option for diagnosing this infection. This study was thus aimed at conventional PCR molecular detection and characterisation of Blastocystis spp. in human faecal samples from Ibagué, Colombia, using primers targeting the small subunit ribosomal ribonucleic acid (rRNA) gene. One hundred human faecal samples with confirmed Blastocystis spp. were studied, revealing the following subtype genetic diversity: ST1 50%, ST2 33% and ST3 17%. The results contributed to the limited information available regarding Blastocystis spp. in Colombia and created a reference point for further studies in the region.http://www.sciencedirect.com/science/article/pii/S2405673120300015ProtozoanIntestinal infectionBlastocystisSubtypingMolecular diagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caterine Potes-Morales Laura A. Osorio-Delgado Julio César Carranza Gustavo Adolfo Vallejo |
spellingShingle |
Caterine Potes-Morales Laura A. Osorio-Delgado Julio César Carranza Gustavo Adolfo Vallejo The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia Parasite Epidemiology and Control Protozoan Intestinal infection Blastocystis Subtyping Molecular diagnosis |
author_facet |
Caterine Potes-Morales Laura A. Osorio-Delgado Julio César Carranza Gustavo Adolfo Vallejo |
author_sort |
Caterine Potes-Morales |
title |
The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia |
title_short |
The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia |
title_full |
The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia |
title_fullStr |
The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia |
title_full_unstemmed |
The first molecular detection of Blastocystis subtypes in human faecal samples from Ibague, Colombia |
title_sort |
first molecular detection of blastocystis subtypes in human faecal samples from ibague, colombia |
publisher |
Elsevier |
series |
Parasite Epidemiology and Control |
issn |
2405-6731 |
publishDate |
2020-05-01 |
description |
Blastocystis spp. has become one of the protozoans arousing the greatest scientific interest because of the controversy surrounding its biology; it is currently considered one of the most prevalent organisms in humans and animals worldwide. Such prevalence increases, especially in tropical countries where infection rates are high, highlighting the need to conduct studies focused on understanding this protozoan's biology. Interestingly, molecular tools are emerging as the best option for diagnosing this infection. This study was thus aimed at conventional PCR molecular detection and characterisation of Blastocystis spp. in human faecal samples from Ibagué, Colombia, using primers targeting the small subunit ribosomal ribonucleic acid (rRNA) gene. One hundred human faecal samples with confirmed Blastocystis spp. were studied, revealing the following subtype genetic diversity: ST1 50%, ST2 33% and ST3 17%. The results contributed to the limited information available regarding Blastocystis spp. in Colombia and created a reference point for further studies in the region. |
topic |
Protozoan Intestinal infection Blastocystis Subtyping Molecular diagnosis |
url |
http://www.sciencedirect.com/science/article/pii/S2405673120300015 |
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