Bacterial Community Characterization in Paper Mill White Water
The paper production process is significantly affected by direct and indirect effects of microorganism proliferation. Microorganisms can be introduced in different steps. Some microorganisms find optimum growth conditions and proliferate along the production process, affecting both the end product q...
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North Carolina State University
2014-03-01
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doaj-48a3db69e7604836a41bda178e6a63202020-11-24T21:58:35ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-03-01922541255910.15376/biores.9.2.2541-2559Bacterial Community Characterization in Paper Mill White WaterCarolina Chiellini0Renato Iannelli1Raissa Lena2Maria Gullo3Giulio Petroni4Department of Biology, unit of Protistology-Zoology, University of Pisa, Via Volta 4, Pisa, Italy CRA Agricultural Research Council, Research Centre for Agrobiology and Pedology (CRA-ABP) (Florence) Piazza D'Azeglio, 30, Florence, ItalyDepartment of Engineering for Energy, Systems, Territory and Construction, University of Pisa, via Gabba 22, Pisa, ItalyDepartment of Biology, unit of Protistology-Zoology, University of Pisa, Via Volta 4, Pisa, ItalyDepartment of Life Science, University of Modena and Reggio Emilia, Via Amendola, 2, Reggio Emilia, ItalyUniversity of PisaThe paper production process is significantly affected by direct and indirect effects of microorganism proliferation. Microorganisms can be introduced in different steps. Some microorganisms find optimum growth conditions and proliferate along the production process, affecting both the end product quality and the production efficiency. The increasing need to reduce water consumption for economic and environmental reasons has led most paper mills to reuse water through increasingly closed cycles, thus exacerbating the bacterial proliferation problem. In this work, microbial communities in a paper mill located in Italy were characterized using both culture-dependent and independent methods. Fingerprinting molecular analysis and 16S rRNA library construction coupled with bacterial isolation were performed. Results highlighted that the bacterial community composition was spatially homogeneous along the whole process, while it was slightly variable over time. The culture-independent approach confirmed the presence of the main bacterial phyla detected with plate counting, coherently with earlier cultivation studies (Proteobacteria, Bacteroidetes, and Firmicutes), but with a higher genus diversification than previously observed. Some minor bacterial groups, not detectable by cultivation, were also detected in the aqueous phase. Overall, the population dynamics observed with the double approach led us to hypothesize a possible role of suspended bacteria in the re-formation mechanisms of resistant biofilms.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_2541_Chiellini_Bacterial_Paper_MillPaper mill16S rRNAIsolationLibraryWhite water |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carolina Chiellini Renato Iannelli Raissa Lena Maria Gullo Giulio Petroni |
spellingShingle |
Carolina Chiellini Renato Iannelli Raissa Lena Maria Gullo Giulio Petroni Bacterial Community Characterization in Paper Mill White Water BioResources Paper mill 16S rRNA Isolation Library White water |
author_facet |
Carolina Chiellini Renato Iannelli Raissa Lena Maria Gullo Giulio Petroni |
author_sort |
Carolina Chiellini |
title |
Bacterial Community Characterization in Paper Mill White Water |
title_short |
Bacterial Community Characterization in Paper Mill White Water |
title_full |
Bacterial Community Characterization in Paper Mill White Water |
title_fullStr |
Bacterial Community Characterization in Paper Mill White Water |
title_full_unstemmed |
Bacterial Community Characterization in Paper Mill White Water |
title_sort |
bacterial community characterization in paper mill white water |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 1930-2126 |
publishDate |
2014-03-01 |
description |
The paper production process is significantly affected by direct and indirect effects of microorganism proliferation. Microorganisms can be introduced in different steps. Some microorganisms find optimum growth conditions and proliferate along the production process, affecting both the end product quality and the production efficiency. The increasing need to reduce water consumption for economic and environmental reasons has led most paper mills to reuse water through increasingly closed cycles, thus exacerbating the bacterial proliferation problem. In this work, microbial communities in a paper mill located in Italy were characterized using both culture-dependent and independent methods. Fingerprinting molecular analysis and 16S rRNA library construction coupled with bacterial isolation were performed. Results highlighted that the bacterial community composition was spatially homogeneous along the whole process, while it was slightly variable over time. The culture-independent approach confirmed the presence of the main bacterial phyla detected with plate counting, coherently with earlier cultivation studies (Proteobacteria, Bacteroidetes, and Firmicutes), but with a higher genus diversification than previously observed. Some minor bacterial groups, not detectable by cultivation, were also detected in the aqueous phase. Overall, the population dynamics observed with the double approach led us to hypothesize a possible role of suspended bacteria in the re-formation mechanisms of resistant biofilms. |
topic |
Paper mill 16S rRNA Isolation Library White water |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_2541_Chiellini_Bacterial_Paper_Mill |
work_keys_str_mv |
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1725851227129905152 |