Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study
Background: Streptococcus pyogenes infection is one of the common upper respiratory infections among children. Human milk has antibacterial properties that may play a role against infections. The study was conducted to observe the antibacterial effect of human milk against Streptococcus pyogenes....
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Universitas Padjadjaran
2019-12-01
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doaj-48a980490b4b4fcaadab8cb5bd211b042020-11-25T01:23:41ZengUniversitas PadjadjaranAlthea Medical Journal2337-43302019-12-016420120410.15850/amj.v6n4.16971153Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro StudyNovi Vicahyani Utami0Nurul Nadiya Binti Abdul Razak1Dzulfikar D. Lukmanul Hakim2Department of Biomedical Sciences Faculty of Medicine Universitas PadjadjaranFaculty of Medicine, Universitas PadjadjaranDepartment of Child Health Faculty of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital BandungBackground: Streptococcus pyogenes infection is one of the common upper respiratory infections among children. Human milk has antibacterial properties that may play a role against infections. The study was conducted to observe the antibacterial effect of human milk against Streptococcus pyogenes. Methods: This was an in vitro experimental study conducted on October–November 2012 in Microbiology Laboratory, Faculty of Medicine, Universitas Padjadjaran, Bandung, using agar well diffusion method. Human milk was collected from eligible donors and tested at concentrations of 70%, 80%, 90%, and 100% dilution with water. Inhibition zone formed surrounding the wells were measured after 24 hours of incubation. Results: All samples incubated with human mils (in concentration of 70%, 80%, 90% and 100%) inhibited the growth of Streptococcus pyogenes, with the inhibition zone mean diameters of 26.33, 28.00, 28.00 and 28.33mm, respectively, compared to the well containing sterile aquadest that did not show any zone of inhibition. Conclusions: Human milk has an in vitro antibacterial effect against Streptococcus pyogenes. Giving human milk to babies is important for preventing infectious diseases.http://journal.fk.unpad.ac.id/index.php/amj/article/view/1697antibacterialhuman milkin vitrostreptococcus pyogenes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Novi Vicahyani Utami Nurul Nadiya Binti Abdul Razak Dzulfikar D. Lukmanul Hakim |
spellingShingle |
Novi Vicahyani Utami Nurul Nadiya Binti Abdul Razak Dzulfikar D. Lukmanul Hakim Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study Althea Medical Journal antibacterial human milk in vitro streptococcus pyogenes |
author_facet |
Novi Vicahyani Utami Nurul Nadiya Binti Abdul Razak Dzulfikar D. Lukmanul Hakim |
author_sort |
Novi Vicahyani Utami |
title |
Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study |
title_short |
Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study |
title_full |
Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study |
title_fullStr |
Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study |
title_full_unstemmed |
Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study |
title_sort |
antibacterial effect of human milk against streptococcus pyogenes: an in vitro study |
publisher |
Universitas Padjadjaran |
series |
Althea Medical Journal |
issn |
2337-4330 |
publishDate |
2019-12-01 |
description |
Background: Streptococcus pyogenes infection is one of the common upper respiratory infections among children. Human milk has antibacterial properties that may play a role against infections. The study was conducted to observe the antibacterial effect of human milk against Streptococcus pyogenes.
Methods: This was an in vitro experimental study conducted on October–November 2012 in Microbiology Laboratory, Faculty of Medicine, Universitas Padjadjaran, Bandung, using agar well diffusion method. Human milk was collected from eligible donors and tested at concentrations of 70%, 80%, 90%, and 100% dilution with water. Inhibition zone formed surrounding the wells were measured after 24 hours of incubation.
Results: All samples incubated with human mils (in concentration of 70%, 80%, 90% and 100%) inhibited the growth of Streptococcus pyogenes, with the inhibition zone mean diameters of 26.33, 28.00, 28.00 and 28.33mm, respectively, compared to the well containing sterile aquadest that did not show any zone of inhibition.
Conclusions: Human milk has an in vitro antibacterial effect against Streptococcus pyogenes. Giving human milk to babies is important for preventing infectious diseases. |
topic |
antibacterial human milk in vitro streptococcus pyogenes |
url |
http://journal.fk.unpad.ac.id/index.php/amj/article/view/1697 |
work_keys_str_mv |
AT novivicahyaniutami antibacterialeffectofhumanmilkagainststreptococcuspyogenesaninvitrostudy AT nurulnadiyabintiabdulrazak antibacterialeffectofhumanmilkagainststreptococcuspyogenesaninvitrostudy AT dzulfikardlukmanulhakim antibacterialeffectofhumanmilkagainststreptococcuspyogenesaninvitrostudy |
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1725120613100552192 |