Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis
<p>Abstract</p> <p>Background</p> <p>A giant congenital melanocytic nevus (GCMN) is a malformation of the pigment cells. It is a distress to the patients for two reasons: one is disfigurement, and the other is the possibility of malignant changes. However, the underlyin...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2012-08-01
|
Series: | Proteome Science |
Subjects: | |
Online Access: | http://www.proteomesci.com/content/10/1/50 |
id |
doaj-48b6a2bdab5f40c7a527957fa24ec062 |
---|---|
record_format |
Article |
spelling |
doaj-48b6a2bdab5f40c7a527957fa24ec0622020-11-24T22:02:43ZengBMCProteome Science1477-59562012-08-011015010.1186/1477-5956-10-50Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesisKim Hyoung KyuKim Yong KyuSong In-SungLee Sung-RyulJeong Seung HunKim Min HeeSeo Dae YunKim NariRhee Byoung DooKo Kyoung SooTark Kwan ChulPark Chul GyooCho Je-YoelHan Jin<p>Abstract</p> <p>Background</p> <p>A giant congenital melanocytic nevus (GCMN) is a malformation of the pigment cells. It is a distress to the patients for two reasons: one is disfigurement, and the other is the possibility of malignant changes. However, the underlying mechanisms of the development of GCMN and melanotumorigenesis in GCMN are unknown. Hence, the aim of this study was to identify the proteomic alterations and associated functional pathways in GCMN.</p> <p>Results</p> <p>Proteomic differences between GCMN (n = 3) and normal skin samples (n = 3) were analyzed by one-dimensional-liquid chromatography-tandem mass spectrometry Relative levels of the selected proteins were validated using western blot analysis. The biological processes associated with the abundance modified proteins were analyzed using bioinformatic tools. Among the 46 abundance modified proteins, expression of 4 proteins was significantly downregulated and expression of 42 proteins was significantly upregulated in GCMN compared to normal skin samples (p < 0.05). More importantly, 31% of the upregulated proteins were implicated in various cancers, with five proteins being specifically related with melanoma. The abundance modified proteins in GCMN were involved in the biological processes of neurotrophin signaling, melanosome, and downregulated of MTA-3 in ER-negative breast tumors. In particular, an increase in the expression of the 14-3-3 protein family members appeared to be associated with key cellular biological functions in GCMN. Western blot analysis confirmed the upregulation of 14-3-3epsilon, 14-3-3 tau, and prohibitin in GCMN.</p> <p>Conclusion</p> <p>These findings suggest that GCMN exhibits potential proteomic alterations, which may play a role in melanotumorigenesis, and the significant alteration of 14-3-3 family proteins could be a key regulator of the biological pathway remodeling in GCMN.</p> http://www.proteomesci.com/content/10/1/50Giant congenital melanocytic neviMelanotumorigenesisProteomics14-3-3 epsilon14-3-3 tauSystemic analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kim Hyoung Kyu Kim Yong Kyu Song In-Sung Lee Sung-Ryul Jeong Seung Hun Kim Min Hee Seo Dae Yun Kim Nari Rhee Byoung Doo Ko Kyoung Soo Tark Kwan Chul Park Chul Gyoo Cho Je-Yoel Han Jin |
spellingShingle |
Kim Hyoung Kyu Kim Yong Kyu Song In-Sung Lee Sung-Ryul Jeong Seung Hun Kim Min Hee Seo Dae Yun Kim Nari Rhee Byoung Doo Ko Kyoung Soo Tark Kwan Chul Park Chul Gyoo Cho Je-Yoel Han Jin Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis Proteome Science Giant congenital melanocytic nevi Melanotumorigenesis Proteomics 14-3-3 epsilon 14-3-3 tau Systemic analysis |
author_facet |
Kim Hyoung Kyu Kim Yong Kyu Song In-Sung Lee Sung-Ryul Jeong Seung Hun Kim Min Hee Seo Dae Yun Kim Nari Rhee Byoung Doo Ko Kyoung Soo Tark Kwan Chul Park Chul Gyoo Cho Je-Yoel Han Jin |
author_sort |
Kim Hyoung Kyu |
title |
Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis |
title_short |
Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis |
title_full |
Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis |
title_fullStr |
Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis |
title_full_unstemmed |
Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis |
title_sort |
human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis |
publisher |
BMC |
series |
Proteome Science |
issn |
1477-5956 |
publishDate |
2012-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>A giant congenital melanocytic nevus (GCMN) is a malformation of the pigment cells. It is a distress to the patients for two reasons: one is disfigurement, and the other is the possibility of malignant changes. However, the underlying mechanisms of the development of GCMN and melanotumorigenesis in GCMN are unknown. Hence, the aim of this study was to identify the proteomic alterations and associated functional pathways in GCMN.</p> <p>Results</p> <p>Proteomic differences between GCMN (n = 3) and normal skin samples (n = 3) were analyzed by one-dimensional-liquid chromatography-tandem mass spectrometry Relative levels of the selected proteins were validated using western blot analysis. The biological processes associated with the abundance modified proteins were analyzed using bioinformatic tools. Among the 46 abundance modified proteins, expression of 4 proteins was significantly downregulated and expression of 42 proteins was significantly upregulated in GCMN compared to normal skin samples (p < 0.05). More importantly, 31% of the upregulated proteins were implicated in various cancers, with five proteins being specifically related with melanoma. The abundance modified proteins in GCMN were involved in the biological processes of neurotrophin signaling, melanosome, and downregulated of MTA-3 in ER-negative breast tumors. In particular, an increase in the expression of the 14-3-3 protein family members appeared to be associated with key cellular biological functions in GCMN. Western blot analysis confirmed the upregulation of 14-3-3epsilon, 14-3-3 tau, and prohibitin in GCMN.</p> <p>Conclusion</p> <p>These findings suggest that GCMN exhibits potential proteomic alterations, which may play a role in melanotumorigenesis, and the significant alteration of 14-3-3 family proteins could be a key regulator of the biological pathway remodeling in GCMN.</p> |
topic |
Giant congenital melanocytic nevi Melanotumorigenesis Proteomics 14-3-3 epsilon 14-3-3 tau Systemic analysis |
url |
http://www.proteomesci.com/content/10/1/50 |
work_keys_str_mv |
AT kimhyoungkyu humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT kimyongkyu humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT songinsung humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT leesungryul humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT jeongseunghun humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT kimminhee humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT seodaeyun humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT kimnari humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT rheebyoungdoo humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT kokyoungsoo humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT tarkkwanchul humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT parkchulgyoo humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT chojeyoel humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis AT hanjin humangiantcongenitalmelanocyticnevusexhibitspotentialproteomicalterationsleadingtomelanotumorigenesis |
_version_ |
1725834308349853696 |