Bioluminescence imaging of leukemia cell lines <it>in vitro</it> and in mouse xenografts: effects of monoclonal and polyclonal cell populations on intensity and kinetics of photon emission

• Main Authors: Christoph Sandra, Schlegel Jennifer, Alvarez-Calderon Francesca, Kim Yong-Mi, Brandao Luis N, DeRyckere Deborah, Graham Douglas K
• Format: Article
• Language: English
• Published: BMC 2013-01-01
• Series: Journal of Hematology & Oncology
• Subjects: Bioluminescence imaging; Monoclonal population; Polyclonal population; Firefly luciferase; Leukemia; Xenograft
• Online Access: http://www.jhoonline.org/content/6/1/10

<p>Abstract</p> <p>Background</p> <p>We investigated the utility of bioluminescence imaging (BLI) using firefly luciferase in monoclonal and polyclonal populations of leukemia cells <it>in vitro</it> and <it>in vivo</it>.</p> <p>Methods</p> <p>Monoclonal and polyclonal human lymphoid and myeloid leukemia cell lines transduced with firefly luciferase were used for BLI.</p> <p>Results</p> <p>Kinetics and dynamics of bioluminescence signal were cell line dependent. Luciferase expression decreased significantly over time in polyclonal leukemia cells <it>in vitro.</it> Transplantation of polyclonal luciferase-tagged cells in mice resulted in inconsistent signal intensity. After selection of monoclonal cell populations, luciferase activity was stable, equal kinetic and dynamic of bioluminescence intensity and strong correlation between cell number and light emission <it>in vitro</it> were observed. We obtained an equal development of leukemia burden detected by luciferase activity in NOD-scid-gamma mice after transplantation of monoclonal populations.</p> <p>Conclusion</p> <p>The use of monoclonal leukemia cells selected for stable and equal luciferase activity is recommended for experiments <it>in vitro</it> and xenograft mouse models. The findings are highly significant for bioluminescence imaging focused on pre-clinical drug development.</p>