Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications

Radial flow perfusion of cell-seeded hollow cylindrical porous scaffolds may overcome the transport limitations of pure diffusion and direct axial perfusion in the realization of bioengineered substitutes of failing or missing tissues. Little has been reported on the optimization criteria of such bi...

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Main Authors:	Danilo Donato, Ilaria E. De Napoli, Gerardo Catapano
Format:	Article
Language:	English
Published:	MDPI AG 2014-01-01
Series:	Processes
Subjects:	bioreactor model oxygen radial flow tissue engineering transport
Online Access:	http://www.mdpi.com/2227-9717/2/1/34

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spelling	doaj-48d1258bc86148728f9d7d541a6e49732020-11-25T01:52:00ZengMDPI AGProcesses2227-97172014-01-0121345710.3390/pr2010034pr2010034Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic ApplicationsDanilo Donato0Ilaria E. De Napoli1Gerardo Catapano2Department of Environmental, Territory and Chemical Engineering, University of Calabria, Via P. Bucci, 87030 Rende (CS), ItalyDepartment of Environmental, Territory and Chemical Engineering, University of Calabria, Via P. Bucci, 87030 Rende (CS), ItalyDepartment of Environmental, Territory and Chemical Engineering, University of Calabria, Via P. Bucci, 87030 Rende (CS), ItalyRadial flow perfusion of cell-seeded hollow cylindrical porous scaffolds may overcome the transport limitations of pure diffusion and direct axial perfusion in the realization of bioengineered substitutes of failing or missing tissues. Little has been reported on the optimization criteria of such bioreactors. A steady-state model was developed, combining convective and dispersive transport of dissolved oxygen with Michaelis-Menten cellular consumption kinetics. Dimensional analysis was used to combine more effectively geometric and operational variables in the dimensionless groups determining bioreactor performance. The effectiveness of cell oxygenation was expressed in terms of non-hypoxic fractional construct volume. The model permits the optimization of the geometry of hollow cylindrical constructs, and direction and magnitude of perfusion flow, to ensure cell oxygenation and culture at controlled oxygen concentration profiles. This may help engineer tissues suitable for therapeutic and drug screening purposes.http://www.mdpi.com/2227-9717/2/1/34bioreactormodeloxygenradial flowtissue engineeringtransport
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Danilo Donato Ilaria E. De Napoli Gerardo Catapano
spellingShingle	Danilo Donato Ilaria E. De Napoli Gerardo Catapano Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications Processes bioreactor model oxygen radial flow tissue engineering transport
author_facet	Danilo Donato Ilaria E. De Napoli Gerardo Catapano
author_sort	Danilo Donato
title	Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications
title_short	Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications
title_full	Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications
title_fullStr	Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications
title_full_unstemmed	Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications
title_sort	model-based optimization of scaffold geometry and operating conditions of radial flow packed-bed bioreactors for therapeutic applications
publisher	MDPI AG
series	Processes
issn	2227-9717
publishDate	2014-01-01
description	Radial flow perfusion of cell-seeded hollow cylindrical porous scaffolds may overcome the transport limitations of pure diffusion and direct axial perfusion in the realization of bioengineered substitutes of failing or missing tissues. Little has been reported on the optimization criteria of such bioreactors. A steady-state model was developed, combining convective and dispersive transport of dissolved oxygen with Michaelis-Menten cellular consumption kinetics. Dimensional analysis was used to combine more effectively geometric and operational variables in the dimensionless groups determining bioreactor performance. The effectiveness of cell oxygenation was expressed in terms of non-hypoxic fractional construct volume. The model permits the optimization of the geometry of hollow cylindrical constructs, and direction and magnitude of perfusion flow, to ensure cell oxygenation and culture at controlled oxygen concentration profiles. This may help engineer tissues suitable for therapeutic and drug screening purposes.
topic	bioreactor model oxygen radial flow tissue engineering transport
url	http://www.mdpi.com/2227-9717/2/1/34
work_keys_str_mv	AT danilodonato modelbasedoptimizationofscaffoldgeometryandoperatingconditionsofradialflowpackedbedbioreactorsfortherapeuticapplications AT ilariaedenapoli modelbasedoptimizationofscaffoldgeometryandoperatingconditionsofradialflowpackedbedbioreactorsfortherapeuticapplications AT gerardocatapano modelbasedoptimizationofscaffoldgeometryandoperatingconditionsofradialflowpackedbedbioreactorsfortherapeuticapplications
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Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications

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