Synchronous onset of oestradiol-17β secretion by Meishan conceptuses
<p>Abstract</p> <p>The response of Meishan conceptuses to an exogenous precursor for oestradiol-17β biosynthesis was investigated <it>in vitro</it>, to determine whether gestational age or morphological stage of development elicit changes in hormone metabolism. Conceptu...
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doaj-48f2e95acc05405ea6c19de4ed6941af2020-11-24T20:54:14ZengBMCReproductive Biology and Endocrinology1477-78272003-02-01111610.1186/1477-7827-1-16Synchronous onset of oestradiol-17β secretion by Meishan conceptusesMiller Samuel JPickard Amanda RAshworth Cheryl J<p>Abstract</p> <p>The response of Meishan conceptuses to an exogenous precursor for oestradiol-17β biosynthesis was investigated <it>in vitro</it>, to determine whether gestational age or morphological stage of development elicit changes in hormone metabolism. Conceptuses were recovered on days 11, 12, 13 or 15 after the onset of oestrus and cultured for 6 hours at 37°C, in the presence or absence of testosterone. On days 12 and 13 after the onset of oestrus spherical conceptuses were recovered from some gilts, whereas others yielded elongated or filamentous conceptuses. All conceptuses recovered on day 15 after oestrus had elongated. The number of cells per individual conceptus increased from days 11 to 13 after the onset of oestrus (<it>P </it>< 0.001), as did conceptus surface area (<it>P </it>= 0.038). Supplementing culture media with testosterone, as a substrate for oestrogen biosynthesis, significantly increased conceptus oestradiol-17β secretion <it>in vitro </it>on days 12, 13 and 15, regardless of whether pre- or post-elongation conceptuses were cultured. However, on day 11 oestradiol-17β was only detected at significant concentrations in the culture media of four testosterone supplemented conceptuses and only one gilt produced conceptuses capable of secreting oestradiol-17β in the absence of testosterone. Therefore, the onset of conceptus oestradiol-17β secretion is apparently limited by the expression of aromatase enzymes that are activated synchronously, irrespective of the stage of morphological development, within Meishan litters. Once established, Meishan conceptus oestradiol-17β secretion <it>in vitro </it>is increased in the presence of exogenous testosterone.</p> http://www.RBEj.com/content/1/1/16pigconceptusaromatasetestosteronemorphology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miller Samuel J Pickard Amanda R Ashworth Cheryl J |
spellingShingle |
Miller Samuel J Pickard Amanda R Ashworth Cheryl J Synchronous onset of oestradiol-17β secretion by Meishan conceptuses Reproductive Biology and Endocrinology pig conceptus aromatase testosterone morphology |
author_facet |
Miller Samuel J Pickard Amanda R Ashworth Cheryl J |
author_sort |
Miller Samuel J |
title |
Synchronous onset of oestradiol-17β secretion by Meishan conceptuses |
title_short |
Synchronous onset of oestradiol-17β secretion by Meishan conceptuses |
title_full |
Synchronous onset of oestradiol-17β secretion by Meishan conceptuses |
title_fullStr |
Synchronous onset of oestradiol-17β secretion by Meishan conceptuses |
title_full_unstemmed |
Synchronous onset of oestradiol-17β secretion by Meishan conceptuses |
title_sort |
synchronous onset of oestradiol-17β secretion by meishan conceptuses |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2003-02-01 |
description |
<p>Abstract</p> <p>The response of Meishan conceptuses to an exogenous precursor for oestradiol-17β biosynthesis was investigated <it>in vitro</it>, to determine whether gestational age or morphological stage of development elicit changes in hormone metabolism. Conceptuses were recovered on days 11, 12, 13 or 15 after the onset of oestrus and cultured for 6 hours at 37°C, in the presence or absence of testosterone. On days 12 and 13 after the onset of oestrus spherical conceptuses were recovered from some gilts, whereas others yielded elongated or filamentous conceptuses. All conceptuses recovered on day 15 after oestrus had elongated. The number of cells per individual conceptus increased from days 11 to 13 after the onset of oestrus (<it>P </it>< 0.001), as did conceptus surface area (<it>P </it>= 0.038). Supplementing culture media with testosterone, as a substrate for oestrogen biosynthesis, significantly increased conceptus oestradiol-17β secretion <it>in vitro </it>on days 12, 13 and 15, regardless of whether pre- or post-elongation conceptuses were cultured. However, on day 11 oestradiol-17β was only detected at significant concentrations in the culture media of four testosterone supplemented conceptuses and only one gilt produced conceptuses capable of secreting oestradiol-17β in the absence of testosterone. Therefore, the onset of conceptus oestradiol-17β secretion is apparently limited by the expression of aromatase enzymes that are activated synchronously, irrespective of the stage of morphological development, within Meishan litters. Once established, Meishan conceptus oestradiol-17β secretion <it>in vitro </it>is increased in the presence of exogenous testosterone.</p> |
topic |
pig conceptus aromatase testosterone morphology |
url |
http://www.RBEj.com/content/1/1/16 |
work_keys_str_mv |
AT millersamuelj synchronousonsetofoestradiol17bsecretionbymeishanconceptuses AT pickardamandar synchronousonsetofoestradiol17bsecretionbymeishanconceptuses AT ashworthcherylj synchronousonsetofoestradiol17bsecretionbymeishanconceptuses |
_version_ |
1716795110794985472 |