Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

Abstract: We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors...

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Main Authors:	Katerina Vlahos, Koula Sourris, Robyn Mayberry, Penelope McDonald, Freya F. Bruveris, Jacqueline V. Schiesser, Kiymet Bozaoglu, Paul J. Lockhart, Edouard G. Stanley, Andrew G. Elefanty
Format:	Article
Language:	English
Published:	Elsevier 2019-01-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506118303155

id	doaj-48f4df3ffb25402b8a472d00f85c9950
record_format	Article
spelling	doaj-48f4df3ffb25402b8a472d00f85c99502020-11-24T22:13:44ZengElsevierStem Cell Research1873-50612019-01-0134Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adultsKaterina Vlahos0Koula Sourris1Robyn Mayberry2Penelope McDonald3Freya F. Bruveris4Jacqueline V. Schiesser5Kiymet Bozaoglu6Paul J. Lockhart7Edouard G. Stanley8Andrew G. Elefanty9Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Corresponding author.Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, AustraliaMurdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, AustraliaAbstract: We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.http://www.sciencedirect.com/science/article/pii/S1873506118303155
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Katerina Vlahos Koula Sourris Robyn Mayberry Penelope McDonald Freya F. Bruveris Jacqueline V. Schiesser Kiymet Bozaoglu Paul J. Lockhart Edouard G. Stanley Andrew G. Elefanty
spellingShingle	Katerina Vlahos Koula Sourris Robyn Mayberry Penelope McDonald Freya F. Bruveris Jacqueline V. Schiesser Kiymet Bozaoglu Paul J. Lockhart Edouard G. Stanley Andrew G. Elefanty Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults Stem Cell Research
author_facet	Katerina Vlahos Koula Sourris Robyn Mayberry Penelope McDonald Freya F. Bruveris Jacqueline V. Schiesser Kiymet Bozaoglu Paul J. Lockhart Edouard G. Stanley Andrew G. Elefanty
author_sort	Katerina Vlahos
title	Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
title_short	Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
title_full	Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
title_fullStr	Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
title_full_unstemmed	Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
title_sort	generation of ipsc lines from peripheral blood mononuclear cells from 5 healthy adults
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2019-01-01
description	Abstract: We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.
url	http://www.sciencedirect.com/science/article/pii/S1873506118303155
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Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

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