Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient
An HIV positive patient presenting a clinical picture of visceral leishmaniasis co-infection was submitted to a bone marrow aspiration after admission to hospital. Amastigotes forms were seen in the bone marrow aspirate and the parasite grew in culture as promastigotes. Molecular analyses showed tha...
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Instituto Oswaldo Cruz, Ministério da Saúde
1998-07-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000400021 |
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doaj-48f8e6242d364764b968502c92fed2f52020-11-24T22:58:51ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601998-07-0193453153710.1590/S0074-02761998000400021Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive PatientRaquel S PachecoMauro CA MarzochiMarize Q PiresCélia MM BritoMaria de Fátima MadeiraElizabeth GO Barbosa-SantosAn HIV positive patient presenting a clinical picture of visceral leishmaniasis co-infection was submitted to a bone marrow aspiration after admission to hospital. Amastigotes forms were seen in the bone marrow aspirate and the parasite grew in culture as promastigotes. Molecular analyses showed that the flagellates isolated did not belong to the genera Leishmania, Trypanosoma or Sauroleishmania. It was not possible to establish infection in laboratory animals. In vitro culture of mouse peritoneal macrophages revealed the invasion of the host cells by the flagellates and their killing 48 hr after infection. Opportunistic infection with an insect trypanosomatid was suspected. Further hybridization analyses against a pannel of different monoxenous and heteroxenous trypanosomatids showed kDNA cross-homology with Leptomonas pulexsimulantis a trypanosomatid found in the dog's fleahttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000400021opportunistic infectionAIDSmonoxenous trypanosomatidminicirclehybridization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raquel S Pacheco Mauro CA Marzochi Marize Q Pires Célia MM Brito Maria de Fátima Madeira Elizabeth GO Barbosa-Santos |
spellingShingle |
Raquel S Pacheco Mauro CA Marzochi Marize Q Pires Célia MM Brito Maria de Fátima Madeira Elizabeth GO Barbosa-Santos Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient Memórias do Instituto Oswaldo Cruz. opportunistic infection AIDS monoxenous trypanosomatid minicircle hybridization |
author_facet |
Raquel S Pacheco Mauro CA Marzochi Marize Q Pires Célia MM Brito Maria de Fátima Madeira Elizabeth GO Barbosa-Santos |
author_sort |
Raquel S Pacheco |
title |
Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient |
title_short |
Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient |
title_full |
Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient |
title_fullStr |
Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient |
title_full_unstemmed |
Parasite Genotypically Related to a Monoxenous Trypanosomatid of Dog's Flea Causing Opportunistic Infection in an HIV Positive Patient |
title_sort |
parasite genotypically related to a monoxenous trypanosomatid of dog's flea causing opportunistic infection in an hiv positive patient |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
1998-07-01 |
description |
An HIV positive patient presenting a clinical picture of visceral leishmaniasis co-infection was submitted to a bone marrow aspiration after admission to hospital. Amastigotes forms were seen in the bone marrow aspirate and the parasite grew in culture as promastigotes. Molecular analyses showed that the flagellates isolated did not belong to the genera Leishmania, Trypanosoma or Sauroleishmania. It was not possible to establish infection in laboratory animals. In vitro culture of mouse peritoneal macrophages revealed the invasion of the host cells by the flagellates and their killing 48 hr after infection. Opportunistic infection with an insect trypanosomatid was suspected. Further hybridization analyses against a pannel of different monoxenous and heteroxenous trypanosomatids showed kDNA cross-homology with Leptomonas pulexsimulantis a trypanosomatid found in the dog's flea |
topic |
opportunistic infection AIDS monoxenous trypanosomatid minicircle hybridization |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000400021 |
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