Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis
<i>Background and Objectives</i>: The clinical manifestations and course of chronic pancreatitis (CP) are often nonspecific and variable, hampering diagnosis of the risk of exocrine pancreatic insufficiency (EPI). Development of new, reproducible, and non-invasive methods to diagnose EPI...
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doaj-490e230c5f8646879502360253bf78a52021-09-26T00:39:10ZengMDPI AGMedicina1010-660X1648-91442021-08-015787687610.3390/medicina57090876Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic PancreatitisCaridad Díaz0Cristina Jiménez-Luna1Carmelo Diéguez-Castillo2Ariadna Martín3José Prados4José Luis Martín-Ruíz5Olga Genilloud6Francisca Vicente7José Pérez del Palacio8Octavio Caba9Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores, 18012 Granada, SpainCenter of Biomedical Research (CIBM), Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, 18012 Granada, SpainDepartment of Gastroenterology, San Cecilio University Hospital, 18012 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores, 18012 Granada, SpainCenter of Biomedical Research (CIBM), Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, 18012 Granada, SpainDepartment of Gastroenterology, San Cecilio University Hospital, 18012 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores, 18012 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores, 18012 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores, 18012 Granada, SpainCenter of Biomedical Research (CIBM), Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, 18012 Granada, Spain<i>Background and Objectives</i>: The clinical manifestations and course of chronic pancreatitis (CP) are often nonspecific and variable, hampering diagnosis of the risk of exocrine pancreatic insufficiency (EPI). Development of new, reproducible, and non-invasive methods to diagnose EPI is therefore a major priority. The objective of this metabolomic study was to identify novel biomarkers associated with EPI. <i>Materials and Methods</i>: We analyzed 53 samples from patients with CP, 32 with and 21 without EPI, using an untargeted metabolomics workflow based on hydrophilic interaction chromatography coupled to high-resolution mass spectrometry. Principal component and partial least squares-discriminant analyses showed significant between-group differentiation, and univariate and multivariate analyses identified potential candidate metabolites that significantly differed between samples from CP patients with EPI and those without EPI. <i>Results</i>: Excellent results were obtained using a six-metabolic panel to diagnose the presence of EPI in CP patients (area under the ROC curve = 0.785). <i>Conclusions</i>: This study confirms the usefulness of metabolomics in this disease setting, allowing the identification of novel biomarkers to differentiate between the presence and absence of EPI in CP patients.https://www.mdpi.com/1648-9144/57/9/876metabolomicsexocrine pancreatic insufficiencychronic pancreatitisbiomarkerdiagnosishigh-resolution mass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caridad Díaz Cristina Jiménez-Luna Carmelo Diéguez-Castillo Ariadna Martín José Prados José Luis Martín-Ruíz Olga Genilloud Francisca Vicente José Pérez del Palacio Octavio Caba |
spellingShingle |
Caridad Díaz Cristina Jiménez-Luna Carmelo Diéguez-Castillo Ariadna Martín José Prados José Luis Martín-Ruíz Olga Genilloud Francisca Vicente José Pérez del Palacio Octavio Caba Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis Medicina metabolomics exocrine pancreatic insufficiency chronic pancreatitis biomarker diagnosis high-resolution mass spectrometry |
author_facet |
Caridad Díaz Cristina Jiménez-Luna Carmelo Diéguez-Castillo Ariadna Martín José Prados José Luis Martín-Ruíz Olga Genilloud Francisca Vicente José Pérez del Palacio Octavio Caba |
author_sort |
Caridad Díaz |
title |
Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis |
title_short |
Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis |
title_full |
Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis |
title_fullStr |
Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis |
title_full_unstemmed |
Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis |
title_sort |
untargeted metabolomics for the diagnosis of exocrine pancreatic insufficiency in chronic pancreatitis |
publisher |
MDPI AG |
series |
Medicina |
issn |
1010-660X 1648-9144 |
publishDate |
2021-08-01 |
description |
<i>Background and Objectives</i>: The clinical manifestations and course of chronic pancreatitis (CP) are often nonspecific and variable, hampering diagnosis of the risk of exocrine pancreatic insufficiency (EPI). Development of new, reproducible, and non-invasive methods to diagnose EPI is therefore a major priority. The objective of this metabolomic study was to identify novel biomarkers associated with EPI. <i>Materials and Methods</i>: We analyzed 53 samples from patients with CP, 32 with and 21 without EPI, using an untargeted metabolomics workflow based on hydrophilic interaction chromatography coupled to high-resolution mass spectrometry. Principal component and partial least squares-discriminant analyses showed significant between-group differentiation, and univariate and multivariate analyses identified potential candidate metabolites that significantly differed between samples from CP patients with EPI and those without EPI. <i>Results</i>: Excellent results were obtained using a six-metabolic panel to diagnose the presence of EPI in CP patients (area under the ROC curve = 0.785). <i>Conclusions</i>: This study confirms the usefulness of metabolomics in this disease setting, allowing the identification of novel biomarkers to differentiate between the presence and absence of EPI in CP patients. |
topic |
metabolomics exocrine pancreatic insufficiency chronic pancreatitis biomarker diagnosis high-resolution mass spectrometry |
url |
https://www.mdpi.com/1648-9144/57/9/876 |
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