Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma
Elevated plasma fibrinogen levels and tumor progression in patients with gastric cancer (GC) have been largely reported. However, distinct fibrinogen chains and domains have different effects on coagulation, inflammation, and angiogenesis. The aim of this study was to characterize fibrinogen β chain...
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doaj-4915360a6be74f2cbc05ace46b8f8d6f2020-11-24T21:52:50ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-03-0119375910.3390/ijms19030759ijms19030759Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric CarcinomaOmbretta Repetto0Stefania Maiero1Raffaella Magris2Gianmaria Miolo3Maria Rita Cozzi4Agostino Steffan5Vincenzo Canzonieri6Renato Cannizzaro7Valli De Re8Facility of Bio-Proteomics, Immunopathology and Cancer Biomarkers, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyGastroenterology, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyGastroenterology, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyMedical Oncology, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyImmunopathology and Cancer Biomarkers, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyImmunopathology and Cancer Biomarkers, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyPathology, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyGastroenterology, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyFacility of Bio-Proteomics, Immunopathology and Cancer Biomarkers, CRO Aviano National Cancer Institute, 33081 Aviano, ItalyElevated plasma fibrinogen levels and tumor progression in patients with gastric cancer (GC) have been largely reported. However, distinct fibrinogen chains and domains have different effects on coagulation, inflammation, and angiogenesis. The aim of this study was to characterize fibrinogen β chain (FGB) in GC tissues. Retrospectively we analyzed the data of matched pairs of normal (N) and malignant tissues (T) of 28 consecutive patients with GC at diagnosis by combining one- and two-dimensional electrophoresis (1DE and 2DE) with immunoblotting and mass spectrometry together with two-dimensional difference in gel electrophoresis (2D-DIGE). 1DE showed bands of the intact FGB at 50 kDa and the cleaved forms containing the fragment D at ~37–40 kDa, which corresponded to 19 spots in 2DE. In particular, spot 402 at ~50 kDa and spots 526 and 548 at ~37 kDa were of interest by showing an increased expression in tumor tissues. A higher content of spot 402 was associated with stomach antrum, while spots 526 and 548 amounts correlated with corpus and high platelet count (>208 × 109/L). The quantification of FGB and cleaved products may help to further characterize the interconnections between GC and platelet/coagulation pathways.http://www.mdpi.com/1422-0067/19/3/759DIGEcomparative proteomicsgastric cancerfibrinogen β chainFGBcoagulationplateletsbiomarker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ombretta Repetto Stefania Maiero Raffaella Magris Gianmaria Miolo Maria Rita Cozzi Agostino Steffan Vincenzo Canzonieri Renato Cannizzaro Valli De Re |
spellingShingle |
Ombretta Repetto Stefania Maiero Raffaella Magris Gianmaria Miolo Maria Rita Cozzi Agostino Steffan Vincenzo Canzonieri Renato Cannizzaro Valli De Re Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma International Journal of Molecular Sciences DIGE comparative proteomics gastric cancer fibrinogen β chain FGB coagulation platelets biomarker |
author_facet |
Ombretta Repetto Stefania Maiero Raffaella Magris Gianmaria Miolo Maria Rita Cozzi Agostino Steffan Vincenzo Canzonieri Renato Cannizzaro Valli De Re |
author_sort |
Ombretta Repetto |
title |
Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma |
title_short |
Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma |
title_full |
Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma |
title_fullStr |
Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma |
title_full_unstemmed |
Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma |
title_sort |
quantitative proteomic approach targeted to fibrinogen β chain in tissue gastric carcinoma |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-03-01 |
description |
Elevated plasma fibrinogen levels and tumor progression in patients with gastric cancer (GC) have been largely reported. However, distinct fibrinogen chains and domains have different effects on coagulation, inflammation, and angiogenesis. The aim of this study was to characterize fibrinogen β chain (FGB) in GC tissues. Retrospectively we analyzed the data of matched pairs of normal (N) and malignant tissues (T) of 28 consecutive patients with GC at diagnosis by combining one- and two-dimensional electrophoresis (1DE and 2DE) with immunoblotting and mass spectrometry together with two-dimensional difference in gel electrophoresis (2D-DIGE). 1DE showed bands of the intact FGB at 50 kDa and the cleaved forms containing the fragment D at ~37–40 kDa, which corresponded to 19 spots in 2DE. In particular, spot 402 at ~50 kDa and spots 526 and 548 at ~37 kDa were of interest by showing an increased expression in tumor tissues. A higher content of spot 402 was associated with stomach antrum, while spots 526 and 548 amounts correlated with corpus and high platelet count (>208 × 109/L). The quantification of FGB and cleaved products may help to further characterize the interconnections between GC and platelet/coagulation pathways. |
topic |
DIGE comparative proteomics gastric cancer fibrinogen β chain FGB coagulation platelets biomarker |
url |
http://www.mdpi.com/1422-0067/19/3/759 |
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