Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i>
Background: Periodontitis is a chronic inflammatory disease associated with a dysbiotic biofilm. Many pathogens have been related with its progression and severity, one of which is <i>Aggregatibacter actinomycetemcomitans</i>, a Gram-negative bacteria with seven serotypes (a–g) according...
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doaj-4925d15050304e4f98d11db3f1895f842021-03-18T00:06:09ZengMDPI AGMicroorganisms2076-26072021-03-01962262210.3390/microorganisms9030622Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i>Daniel Betancur0Camila Muñoz Grez1Angel Oñate2Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, ChileLaboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, ChileLaboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, ChileBackground: Periodontitis is a chronic inflammatory disease associated with a dysbiotic biofilm. Many pathogens have been related with its progression and severity, one of which is <i>Aggregatibacter actinomycetemcomitans</i>, a Gram-negative bacteria with seven serotypes (a–g) according with the structure of its LPS, with serotype b defined as the most virulent compared with the other serotypes. The aim of this study was to evaluate the response of oral keratinocytes and macrophages to <i>A. actinomycetemcomitans</i>. Methods: Oral keratinocytes (OKF6/TERT2) and macrophages (THP-1) were infected with <i>A. actinomycetemcomitans</i> serotypes a, b and c. The expression of IL-1β, IL-6, IL-8, IL-18, TNF-α, MMP-9, RANKL, TLR-2, TLR-4, TLR-6, thymic stromal lymphopoietin (TSLP), and ICAM-1 was evaluated by qPCR at 2 and 24 h after infection. Results: An increase in the expression of these molecules was induced by all serotypes at both times of infection, with macrophages showing higher levels of expression at 24 h compared to epithelial cells in which the highest levels were observed in the first hours after infection. Conclusions: Keratinocytes and macrophages contribute to the inflammation in periodontitis from the early stages of infection, producing the first waves of cytokines, acting as the first signal for professional immune cell recruitment and modulation of more specific immune responses.https://www.mdpi.com/2076-2607/9/3/622cytokinesepithelial cellskeratinocytesmacrophages<i>Aggregatibacter actinomycetemcomitans</i>periodontitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel Betancur Camila Muñoz Grez Angel Oñate |
spellingShingle |
Daniel Betancur Camila Muñoz Grez Angel Oñate Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i> Microorganisms cytokines epithelial cells keratinocytes macrophages <i>Aggregatibacter actinomycetemcomitans</i> periodontitis |
author_facet |
Daniel Betancur Camila Muñoz Grez Angel Oñate |
author_sort |
Daniel Betancur |
title |
Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i> |
title_short |
Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i> |
title_full |
Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i> |
title_fullStr |
Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i> |
title_full_unstemmed |
Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of <i>Aggregatibacter actinomycetemcomitans</i> |
title_sort |
comparative analysis of cytokine expression in oral keratinocytes and thp-1 macrophages in response to the most prevalent serotypes of <i>aggregatibacter actinomycetemcomitans</i> |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-03-01 |
description |
Background: Periodontitis is a chronic inflammatory disease associated with a dysbiotic biofilm. Many pathogens have been related with its progression and severity, one of which is <i>Aggregatibacter actinomycetemcomitans</i>, a Gram-negative bacteria with seven serotypes (a–g) according with the structure of its LPS, with serotype b defined as the most virulent compared with the other serotypes. The aim of this study was to evaluate the response of oral keratinocytes and macrophages to <i>A. actinomycetemcomitans</i>. Methods: Oral keratinocytes (OKF6/TERT2) and macrophages (THP-1) were infected with <i>A. actinomycetemcomitans</i> serotypes a, b and c. The expression of IL-1β, IL-6, IL-8, IL-18, TNF-α, MMP-9, RANKL, TLR-2, TLR-4, TLR-6, thymic stromal lymphopoietin (TSLP), and ICAM-1 was evaluated by qPCR at 2 and 24 h after infection. Results: An increase in the expression of these molecules was induced by all serotypes at both times of infection, with macrophages showing higher levels of expression at 24 h compared to epithelial cells in which the highest levels were observed in the first hours after infection. Conclusions: Keratinocytes and macrophages contribute to the inflammation in periodontitis from the early stages of infection, producing the first waves of cytokines, acting as the first signal for professional immune cell recruitment and modulation of more specific immune responses. |
topic |
cytokines epithelial cells keratinocytes macrophages <i>Aggregatibacter actinomycetemcomitans</i> periodontitis |
url |
https://www.mdpi.com/2076-2607/9/3/622 |
work_keys_str_mv |
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