Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a difficult enzyme to work with. Here, the authors covalently immobilized it in a microfluidic reactor to enhance its storage/thermal stabilities and reusability, which enabled the continuous artificial synthesis of glucose precursor.

Bibliographic Details
Main Authors:	Yujiao Zhu, Ziyu Huang, Qingming Chen, Qian Wu, Xiaowen Huang, Pui-Kin So, Liyang Shao, Zhongping Yao, Yanwei Jia, Zhaohui Li, Weixing Yu, Yi Yang, Aoqun Jian, Shengbo Sang, Wendong Zhang, Xuming Zhang
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-09-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-12089-6

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spelling	doaj-49316f1752514a8e82b25f6989e0f7d22021-05-11T11:48:08ZengNature Publishing GroupNature Communications2041-17232019-09-011011910.1038/s41467-019-12089-6Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactorsYujiao Zhu0Ziyu Huang1Qingming Chen2Qian Wu3Xiaowen Huang4Pui-Kin So5Liyang Shao6Zhongping Yao7Yanwei Jia8Zhaohui Li9Weixing Yu10Yi Yang11Aoqun Jian12Shengbo Sang13Wendong Zhang14Xuming Zhang15Department of Applied Physics, The Hong Kong Polytechnic UniversityState Key Laboratory of Biobased Material and Green Papermaking; Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)Department of Applied Physics, The Hong Kong Polytechnic UniversityState Key Laboratory for Chemical Biology and Drug Discovery, Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic UniversityState Key Laboratory of Biobased Material and Green Papermaking; Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)University of Research Facility in Life Sciences, The Hong Kong Polytechnic UniversityDepartment of Electrical and Electronic Engineering, Southern University of Science and TechnologyState Key Laboratory for Chemical Biology and Drug Discovery, Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic UniversityState Key Laboratory of Analog and Mixed Signal VLSI, Institute of Microelectronics, University of MacauSchool of Electronics and Information Engineering, Sun Yat-Sen UniversityKey Laboratory of Spectral Imaging Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of SciencesSchool of Physics & Technology, Wuhan UniversityMircoNano System Research Center, College of Information and Computer Science, Taiyuan University of TechnologyMircoNano System Research Center, College of Information and Computer Science, Taiyuan University of TechnologyMircoNano System Research Center, College of Information and Computer Science, Taiyuan University of TechnologyDepartment of Applied Physics, The Hong Kong Polytechnic UniversityRibulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a difficult enzyme to work with. Here, the authors covalently immobilized it in a microfluidic reactor to enhance its storage/thermal stabilities and reusability, which enabled the continuous artificial synthesis of glucose precursor.https://doi.org/10.1038/s41467-019-12089-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yujiao Zhu Ziyu Huang Qingming Chen Qian Wu Xiaowen Huang Pui-Kin So Liyang Shao Zhongping Yao Yanwei Jia Zhaohui Li Weixing Yu Yi Yang Aoqun Jian Shengbo Sang Wendong Zhang Xuming Zhang
spellingShingle	Yujiao Zhu Ziyu Huang Qingming Chen Qian Wu Xiaowen Huang Pui-Kin So Liyang Shao Zhongping Yao Yanwei Jia Zhaohui Li Weixing Yu Yi Yang Aoqun Jian Shengbo Sang Wendong Zhang Xuming Zhang Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors Nature Communications
author_facet	Yujiao Zhu Ziyu Huang Qingming Chen Qian Wu Xiaowen Huang Pui-Kin So Liyang Shao Zhongping Yao Yanwei Jia Zhaohui Li Weixing Yu Yi Yang Aoqun Jian Shengbo Sang Wendong Zhang Xuming Zhang
author_sort	Yujiao Zhu
title	Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
title_short	Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
title_full	Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
title_fullStr	Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
title_full_unstemmed	Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
title_sort	continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-09-01
description	Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a difficult enzyme to work with. Here, the authors covalently immobilized it in a microfluidic reactor to enhance its storage/thermal stabilities and reusability, which enabled the continuous artificial synthesis of glucose precursor.
url	https://doi.org/10.1038/s41467-019-12089-6
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Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors

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