Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
Xanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there are many unknown aspects related to regulatory mechanisms on the synthesis of carotenoids in this yeast. Our recent studies showed that ch...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-03-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00586/full |
id |
doaj-4935d0d4d3a04ec390fd72ac3b20dba4 |
---|---|
record_format |
Article |
spelling |
doaj-4935d0d4d3a04ec390fd72ac3b20dba42020-11-25T02:26:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-03-011010.3389/fmicb.2019.00586450427Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhousMaría Soledad Gutiérrez0Sebastián Campusano1Ana María González2Melissa Gómez3Salvador Barahona4Dionisia Sepúlveda5Peter J. Espenshade6María Fernández-Lobato7Marcelo Baeza8Marcelo Baeza9Víctor Cifuentes10Víctor Cifuentes11Jennifer Alcaíno12Jennifer Alcaíno13Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesCentro de Biologiìa Molecular Severo Ochoa, Departamento de Biologiìa Molecular (UAM-CSIC), Universidad Autoìnoma de Madrid, Madrid, SpainDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileXanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there are many unknown aspects related to regulatory mechanisms on the synthesis of carotenoids in this yeast. Our recent studies showed that changes in sterol levels and composition resulted in upregulation of genes in the mevalonate pathway required for the synthesis of carotenoid precursors, leading to increased production of these pigments. Sterol Regulatory Element-Binding Proteins (SREBP), called Sre1 in yeast, are conserved transcriptional regulators of sterol homeostasis and other cellular processes. Given the results linking sterols and carotenoids, we investigated the role of SREBP in sterol and carotenoid synthesis in X.dendrorhous. In this study, we present the identification and functional characterization of the X.dendrorhousSRE1 gene, which encodes the transcription factor Sre1. The deduced protein has the characteristic features of SREBP/Sre1 and binds to consensus DNA sequences in vitro. RNA-seq analysis and chromatin-immunoprecipitation experiments showed that genes of the mevalonate pathway and ergosterol biosynthesis are directly regulated by Sre1. The sre1- mutation reduced sterol and carotenoid production in X. dendrorhous, and expression of the Sre1 N-terminal domain (Sre1N) increased carotenoid production more than twofold compared to wild-type. Overall, our results indicate that in X. dendrorhous transcriptional regulation of genes in the mevalonate pathway control production of the isoprenoid derivatives, carotenoids and sterol. Our results provide new insights into the conserved regulatory functions of SREBP/Sre1 and identify pointing to the SREBP pathway as a potential target to enhance carotenoid production in X. dendrorhous.https://www.frontiersin.org/article/10.3389/fmicb.2019.00586/fullX. dendrorhousSREBP/Sre1carotenogenesisastaxanthinsterolsergosterol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Soledad Gutiérrez Sebastián Campusano Ana María González Melissa Gómez Salvador Barahona Dionisia Sepúlveda Peter J. Espenshade María Fernández-Lobato Marcelo Baeza Marcelo Baeza Víctor Cifuentes Víctor Cifuentes Jennifer Alcaíno Jennifer Alcaíno |
spellingShingle |
María Soledad Gutiérrez Sebastián Campusano Ana María González Melissa Gómez Salvador Barahona Dionisia Sepúlveda Peter J. Espenshade María Fernández-Lobato Marcelo Baeza Marcelo Baeza Víctor Cifuentes Víctor Cifuentes Jennifer Alcaíno Jennifer Alcaíno Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous Frontiers in Microbiology X. dendrorhous SREBP/Sre1 carotenogenesis astaxanthin sterols ergosterol |
author_facet |
María Soledad Gutiérrez Sebastián Campusano Ana María González Melissa Gómez Salvador Barahona Dionisia Sepúlveda Peter J. Espenshade María Fernández-Lobato Marcelo Baeza Marcelo Baeza Víctor Cifuentes Víctor Cifuentes Jennifer Alcaíno Jennifer Alcaíno |
author_sort |
María Soledad Gutiérrez |
title |
Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous |
title_short |
Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous |
title_full |
Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous |
title_fullStr |
Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous |
title_full_unstemmed |
Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous |
title_sort |
sterol regulatory element-binding protein (sre1) promotes the synthesis of carotenoids and sterols in xanthophyllomyces dendrorhous |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-03-01 |
description |
Xanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there are many unknown aspects related to regulatory mechanisms on the synthesis of carotenoids in this yeast. Our recent studies showed that changes in sterol levels and composition resulted in upregulation of genes in the mevalonate pathway required for the synthesis of carotenoid precursors, leading to increased production of these pigments. Sterol Regulatory Element-Binding Proteins (SREBP), called Sre1 in yeast, are conserved transcriptional regulators of sterol homeostasis and other cellular processes. Given the results linking sterols and carotenoids, we investigated the role of SREBP in sterol and carotenoid synthesis in X.dendrorhous. In this study, we present the identification and functional characterization of the X.dendrorhousSRE1 gene, which encodes the transcription factor Sre1. The deduced protein has the characteristic features of SREBP/Sre1 and binds to consensus DNA sequences in vitro. RNA-seq analysis and chromatin-immunoprecipitation experiments showed that genes of the mevalonate pathway and ergosterol biosynthesis are directly regulated by Sre1. The sre1- mutation reduced sterol and carotenoid production in X. dendrorhous, and expression of the Sre1 N-terminal domain (Sre1N) increased carotenoid production more than twofold compared to wild-type. Overall, our results indicate that in X. dendrorhous transcriptional regulation of genes in the mevalonate pathway control production of the isoprenoid derivatives, carotenoids and sterol. Our results provide new insights into the conserved regulatory functions of SREBP/Sre1 and identify pointing to the SREBP pathway as a potential target to enhance carotenoid production in X. dendrorhous. |
topic |
X. dendrorhous SREBP/Sre1 carotenogenesis astaxanthin sterols ergosterol |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.00586/full |
work_keys_str_mv |
AT mariasoledadgutierrez sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT sebastiancampusano sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT anamariagonzalez sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT melissagomez sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT salvadorbarahona sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT dionisiasepulveda sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT peterjespenshade sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT mariafernandezlobato sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT marcelobaeza sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT marcelobaeza sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT victorcifuentes sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT victorcifuentes sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT jenniferalcaino sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous AT jenniferalcaino sterolregulatoryelementbindingproteinsre1promotesthesynthesisofcarotenoidsandsterolsinxanthophyllomycesdendrorhous |
_version_ |
1724848929334362112 |