Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous

Xanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there are many unknown aspects related to regulatory mechanisms on the synthesis of carotenoids in this yeast. Our recent studies showed that ch...

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Main Authors: María Soledad Gutiérrez, Sebastián Campusano, Ana María González, Melissa Gómez, Salvador Barahona, Dionisia Sepúlveda, Peter J. Espenshade, María Fernández-Lobato, Marcelo Baeza, Víctor Cifuentes, Jennifer Alcaíno
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00586/full
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spelling doaj-4935d0d4d3a04ec390fd72ac3b20dba42020-11-25T02:26:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-03-011010.3389/fmicb.2019.00586450427Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhousMaría Soledad Gutiérrez0Sebastián Campusano1Ana María González2Melissa Gómez3Salvador Barahona4Dionisia Sepúlveda5Peter J. Espenshade6María Fernández-Lobato7Marcelo Baeza8Marcelo Baeza9Víctor Cifuentes10Víctor Cifuentes11Jennifer Alcaíno12Jennifer Alcaíno13Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesCentro de Biologiìa Molecular Severo Ochoa, Departamento de Biologiìa Molecular (UAM-CSIC), Universidad Autoìnoma de Madrid, Madrid, SpainDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCentro de Biotecnología, Facultad de Ciencias, Universidad de Chile, Santiago, ChileXanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there are many unknown aspects related to regulatory mechanisms on the synthesis of carotenoids in this yeast. Our recent studies showed that changes in sterol levels and composition resulted in upregulation of genes in the mevalonate pathway required for the synthesis of carotenoid precursors, leading to increased production of these pigments. Sterol Regulatory Element-Binding Proteins (SREBP), called Sre1 in yeast, are conserved transcriptional regulators of sterol homeostasis and other cellular processes. Given the results linking sterols and carotenoids, we investigated the role of SREBP in sterol and carotenoid synthesis in X.dendrorhous. In this study, we present the identification and functional characterization of the X.dendrorhousSRE1 gene, which encodes the transcription factor Sre1. The deduced protein has the characteristic features of SREBP/Sre1 and binds to consensus DNA sequences in vitro. RNA-seq analysis and chromatin-immunoprecipitation experiments showed that genes of the mevalonate pathway and ergosterol biosynthesis are directly regulated by Sre1. The sre1- mutation reduced sterol and carotenoid production in X. dendrorhous, and expression of the Sre1 N-terminal domain (Sre1N) increased carotenoid production more than twofold compared to wild-type. Overall, our results indicate that in X. dendrorhous transcriptional regulation of genes in the mevalonate pathway control production of the isoprenoid derivatives, carotenoids and sterol. Our results provide new insights into the conserved regulatory functions of SREBP/Sre1 and identify pointing to the SREBP pathway as a potential target to enhance carotenoid production in X. dendrorhous.https://www.frontiersin.org/article/10.3389/fmicb.2019.00586/fullX. dendrorhousSREBP/Sre1carotenogenesisastaxanthinsterolsergosterol
collection DOAJ
language English
format Article
sources DOAJ
author María Soledad Gutiérrez
Sebastián Campusano
Ana María González
Melissa Gómez
Salvador Barahona
Dionisia Sepúlveda
Peter J. Espenshade
María Fernández-Lobato
Marcelo Baeza
Marcelo Baeza
Víctor Cifuentes
Víctor Cifuentes
Jennifer Alcaíno
Jennifer Alcaíno
spellingShingle María Soledad Gutiérrez
Sebastián Campusano
Ana María González
Melissa Gómez
Salvador Barahona
Dionisia Sepúlveda
Peter J. Espenshade
María Fernández-Lobato
Marcelo Baeza
Marcelo Baeza
Víctor Cifuentes
Víctor Cifuentes
Jennifer Alcaíno
Jennifer Alcaíno
Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
Frontiers in Microbiology
X. dendrorhous
SREBP/Sre1
carotenogenesis
astaxanthin
sterols
ergosterol
author_facet María Soledad Gutiérrez
Sebastián Campusano
Ana María González
Melissa Gómez
Salvador Barahona
Dionisia Sepúlveda
Peter J. Espenshade
María Fernández-Lobato
Marcelo Baeza
Marcelo Baeza
Víctor Cifuentes
Víctor Cifuentes
Jennifer Alcaíno
Jennifer Alcaíno
author_sort María Soledad Gutiérrez
title Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
title_short Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
title_full Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
title_fullStr Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
title_full_unstemmed Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols in Xanthophyllomyces dendrorhous
title_sort sterol regulatory element-binding protein (sre1) promotes the synthesis of carotenoids and sterols in xanthophyllomyces dendrorhous
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2019-03-01
description Xanthophyllomyces dendrorhous is a basidiomycete yeast that synthesizes carotenoids, mainly astaxanthin, which are of great commercial interest. Currently, there are many unknown aspects related to regulatory mechanisms on the synthesis of carotenoids in this yeast. Our recent studies showed that changes in sterol levels and composition resulted in upregulation of genes in the mevalonate pathway required for the synthesis of carotenoid precursors, leading to increased production of these pigments. Sterol Regulatory Element-Binding Proteins (SREBP), called Sre1 in yeast, are conserved transcriptional regulators of sterol homeostasis and other cellular processes. Given the results linking sterols and carotenoids, we investigated the role of SREBP in sterol and carotenoid synthesis in X.dendrorhous. In this study, we present the identification and functional characterization of the X.dendrorhousSRE1 gene, which encodes the transcription factor Sre1. The deduced protein has the characteristic features of SREBP/Sre1 and binds to consensus DNA sequences in vitro. RNA-seq analysis and chromatin-immunoprecipitation experiments showed that genes of the mevalonate pathway and ergosterol biosynthesis are directly regulated by Sre1. The sre1- mutation reduced sterol and carotenoid production in X. dendrorhous, and expression of the Sre1 N-terminal domain (Sre1N) increased carotenoid production more than twofold compared to wild-type. Overall, our results indicate that in X. dendrorhous transcriptional regulation of genes in the mevalonate pathway control production of the isoprenoid derivatives, carotenoids and sterol. Our results provide new insights into the conserved regulatory functions of SREBP/Sre1 and identify pointing to the SREBP pathway as a potential target to enhance carotenoid production in X. dendrorhous.
topic X. dendrorhous
SREBP/Sre1
carotenogenesis
astaxanthin
sterols
ergosterol
url https://www.frontiersin.org/article/10.3389/fmicb.2019.00586/full
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