The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat
BackgroundExperimental studies have shown that gabapentin can reduce neuronal injury in the setting of cerebral ischemia, but the mechanisms have not yet been clearly determined. This study was conducted to determine whether gabapentin pretreatment altered expression levels of heat shock protein 70...
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doaj-493e61489d3e46ceaeff80c012d235272020-11-25T03:10:07ZengKorean Society of AnesthesiologistsKorean Journal of Anesthesiology2005-64192005-75632010-02-0158218419010.4097/kjae.2010.58.2.1846847The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the ratYoo-kyung Kim0Jeong-Gill Leem1Ji-Yeon Sim2Sung-moon Jeong3Kyoung-Woon Joung4Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.BackgroundExperimental studies have shown that gabapentin can reduce neuronal injury in the setting of cerebral ischemia, but the mechanisms have not yet been clearly determined. This study was conducted to determine whether gabapentin pretreatment altered expression levels of heat shock protein 70 and reduced acute phase neuronal injury in rats subjected to transient focal cerebral ischemia/reperfusion.MethodsForty male Sprague-Dawley rats (260-300 g) were randomly assigned to one of four groups (saline-treated, or 0.1, 0.5, or 5 mg/kg gabapentin group). In all animals, focal cerebral ischemia was induced by intraluminal middle cerebral artery occlusion for 1 hour. The animals of the gabapentin groups were pretreated with a single intravenous administration of gabapentin 20 minutes before ischemic insults. The infarct volume, brain edema and motor behavior deficits were analyzed 24 hours after ischemic insult. Caspase-3-reactive cells and cells showing Hsp70 activity were counted in the caudoputamen and fronto-parietal cortex.ResultsThe infarction ratio was significantly decreased in the 5 mg/kg gabapentin group (P < 0.05) and brain edema ratios were significantly reduced in the 0.1 mg/kg, 0.5 mg/kg, and 5 mg/kg gabapentin groups 24 hours after ischemia/reperfusion injury (P < 0.05). There were more Hsp70-reactive cells in the 5 mg/kg gabapentin group than in the saline group in both the caudoputamen and fronto-parietal cortex (P < 0.05).ConclusionsThese results indicate that gabapentin may have a neuroprotective effect and can reduce early neuronal injury caused by focal cerebral ischemia/reperfusion; this may be mediated by expression of Hsp70. However, gabapentin pretreatment did not prevent caspase-dependent apoptosis.http://ekja.org/upload/pdf/kjae-58-184.pdfcaspase-3focal cerebral ischemia/reperfusiongabapentinheat shock protein 70rat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoo-kyung Kim Jeong-Gill Leem Ji-Yeon Sim Sung-moon Jeong Kyoung-Woon Joung |
spellingShingle |
Yoo-kyung Kim Jeong-Gill Leem Ji-Yeon Sim Sung-moon Jeong Kyoung-Woon Joung The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat Korean Journal of Anesthesiology caspase-3 focal cerebral ischemia/reperfusion gabapentin heat shock protein 70 rat |
author_facet |
Yoo-kyung Kim Jeong-Gill Leem Ji-Yeon Sim Sung-moon Jeong Kyoung-Woon Joung |
author_sort |
Yoo-kyung Kim |
title |
The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat |
title_short |
The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat |
title_full |
The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat |
title_fullStr |
The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat |
title_full_unstemmed |
The effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat |
title_sort |
effects of gabapentin pretreatment on brain injury induced by focal cerebral ischemia/reperfusion in the rat |
publisher |
Korean Society of Anesthesiologists |
series |
Korean Journal of Anesthesiology |
issn |
2005-6419 2005-7563 |
publishDate |
2010-02-01 |
description |
BackgroundExperimental studies have shown that gabapentin can reduce neuronal injury in the setting of cerebral ischemia, but the mechanisms have not yet been clearly determined. This study was conducted to determine whether gabapentin pretreatment altered expression levels of heat shock protein 70 and reduced acute phase neuronal injury in rats subjected to transient focal cerebral ischemia/reperfusion.MethodsForty male Sprague-Dawley rats (260-300 g) were randomly assigned to one of four groups (saline-treated, or 0.1, 0.5, or 5 mg/kg gabapentin group). In all animals, focal cerebral ischemia was induced by intraluminal middle cerebral artery occlusion for 1 hour. The animals of the gabapentin groups were pretreated with a single intravenous administration of gabapentin 20 minutes before ischemic insults. The infarct volume, brain edema and motor behavior deficits were analyzed 24 hours after ischemic insult. Caspase-3-reactive cells and cells showing Hsp70 activity were counted in the caudoputamen and fronto-parietal cortex.ResultsThe infarction ratio was significantly decreased in the 5 mg/kg gabapentin group (P < 0.05) and brain edema ratios were significantly reduced in the 0.1 mg/kg, 0.5 mg/kg, and 5 mg/kg gabapentin groups 24 hours after ischemia/reperfusion injury (P < 0.05). There were more Hsp70-reactive cells in the 5 mg/kg gabapentin group than in the saline group in both the caudoputamen and fronto-parietal cortex (P < 0.05).ConclusionsThese results indicate that gabapentin may have a neuroprotective effect and can reduce early neuronal injury caused by focal cerebral ischemia/reperfusion; this may be mediated by expression of Hsp70. However, gabapentin pretreatment did not prevent caspase-dependent apoptosis. |
topic |
caspase-3 focal cerebral ischemia/reperfusion gabapentin heat shock protein 70 rat |
url |
http://ekja.org/upload/pdf/kjae-58-184.pdf |
work_keys_str_mv |
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