Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway
Abstract Background The combination of Radix Paeoniae Alba (RPA) and Rhizoma Atractylodis Macrocephalae (RAM) has long been used as a classic herb pair for the treatment of gynecologic and gastrointestinal diseases, but the underlying mechanisms of the herb pair remain unknown. This study aims to ex...
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doaj-494807271279407da37024df9729dc482020-11-25T02:38:29ZengBMCChinese Medicine1749-85462019-01-0114111110.1186/s13020-019-0224-2Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathwayYangyang Zhou0Hongxun Tao1Anqi Wang2Zhangfeng Zhong3Xu Wu4Mei Wang5Zhaoxiang Bian6Shengpeng Wang7Yitao Wang8State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauLaboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical UniversityLeiden University European Center for Chinese Medicine and Natural Compounds, Institute of Biology, Leiden UniversitySchool of Chinese Medicine, Hong Kong Baptist UniversityState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauAbstract Background The combination of Radix Paeoniae Alba (RPA) and Rhizoma Atractylodis Macrocephalae (RAM) has long been used as a classic herb pair for the treatment of gynecologic and gastrointestinal diseases, but the underlying mechanisms of the herb pair remain unknown. This study aims to explore the anti-inflammatory potentials of RPA–RAM herb pair and to elucidate the underlying mechanisms. Methods The bioactive parts of RPA–RAM were extracted and screened through the inhibitory effects against nitric oxide (NO) production. The effects of optimized RPA–RAM extracts (OPAE) on inflammation-associated mediators were investigated by Western blotting, real-time quantitative PCR (RT-qPCR), Enzyme-linked immunosorbent (ELISA) and immunofluorescence staining. Results OPAE potently suppressed the productions of NO, TNF-α, IL-6 and MCP-1 in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages, concentration-dependently inhibited protein level of inducible nitric oxide synthase (iNOS), dramatically downregulated mRNA expression of iNOS, TNF-α, IL-6 and MCP-1. In addition, OPAE significantly prevented phosphorylation and degradation of inhibitory kappa Bα (IκBα) and subsequently restrained the nuclear translocation of NF-κB p65. Pretreatment with OPAE also attenuated the LPS-induced phosphorylation of ERK, JNK and p38. Conclusions Our findings demonstrated that OPAE suppressed inflammatory responses in LPS-stimulated RAW 264.7 macrophages by decreasing critical molecules involved in MAPK and NF-κB pathway, suggesting that the herb pair could be a promising therapeutic candidate for inflammation-related diseases.http://link.springer.com/article/10.1186/s13020-019-0224-2Radix Paeoniae AlbaRhizoma Atractylodis MacrocephalaeHerb pairInflammatory |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yangyang Zhou Hongxun Tao Anqi Wang Zhangfeng Zhong Xu Wu Mei Wang Zhaoxiang Bian Shengpeng Wang Yitao Wang |
spellingShingle |
Yangyang Zhou Hongxun Tao Anqi Wang Zhangfeng Zhong Xu Wu Mei Wang Zhaoxiang Bian Shengpeng Wang Yitao Wang Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway Chinese Medicine Radix Paeoniae Alba Rhizoma Atractylodis Macrocephalae Herb pair Inflammatory |
author_facet |
Yangyang Zhou Hongxun Tao Anqi Wang Zhangfeng Zhong Xu Wu Mei Wang Zhaoxiang Bian Shengpeng Wang Yitao Wang |
author_sort |
Yangyang Zhou |
title |
Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway |
title_short |
Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway |
title_full |
Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway |
title_fullStr |
Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway |
title_full_unstemmed |
Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway |
title_sort |
chinese herb pair paeoniae radix alba and atractylodis macrocephalae rhizoma suppresses lps-induced inflammatory response through inhibiting mapk and nf-κb pathway |
publisher |
BMC |
series |
Chinese Medicine |
issn |
1749-8546 |
publishDate |
2019-01-01 |
description |
Abstract Background The combination of Radix Paeoniae Alba (RPA) and Rhizoma Atractylodis Macrocephalae (RAM) has long been used as a classic herb pair for the treatment of gynecologic and gastrointestinal diseases, but the underlying mechanisms of the herb pair remain unknown. This study aims to explore the anti-inflammatory potentials of RPA–RAM herb pair and to elucidate the underlying mechanisms. Methods The bioactive parts of RPA–RAM were extracted and screened through the inhibitory effects against nitric oxide (NO) production. The effects of optimized RPA–RAM extracts (OPAE) on inflammation-associated mediators were investigated by Western blotting, real-time quantitative PCR (RT-qPCR), Enzyme-linked immunosorbent (ELISA) and immunofluorescence staining. Results OPAE potently suppressed the productions of NO, TNF-α, IL-6 and MCP-1 in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages, concentration-dependently inhibited protein level of inducible nitric oxide synthase (iNOS), dramatically downregulated mRNA expression of iNOS, TNF-α, IL-6 and MCP-1. In addition, OPAE significantly prevented phosphorylation and degradation of inhibitory kappa Bα (IκBα) and subsequently restrained the nuclear translocation of NF-κB p65. Pretreatment with OPAE also attenuated the LPS-induced phosphorylation of ERK, JNK and p38. Conclusions Our findings demonstrated that OPAE suppressed inflammatory responses in LPS-stimulated RAW 264.7 macrophages by decreasing critical molecules involved in MAPK and NF-κB pathway, suggesting that the herb pair could be a promising therapeutic candidate for inflammation-related diseases. |
topic |
Radix Paeoniae Alba Rhizoma Atractylodis Macrocephalae Herb pair Inflammatory |
url |
http://link.springer.com/article/10.1186/s13020-019-0224-2 |
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