High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues.
Accumulating data have shown the involvement of microRNAs (miRNAs) in endometriosis pathogenesis. In this study, we used a novel approach to determine the endometriotic lesion-specific miRNAs by high-throughput small RNA sequencing of paired samples of peritoneal endometriotic lesions and matched he...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4227690?pdf=render |
id |
doaj-494b0674edc84e3dbc1e10e6a82a276d |
---|---|
record_format |
Article |
spelling |
doaj-494b0674edc84e3dbc1e10e6a82a276d2020-11-24T21:38:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11263010.1371/journal.pone.0112630High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues.Merli SaareKadri RekkerTriin Laisk-PodarDeniss SõritsaAnne Mari RoostJaak SimmAgne Velthut-MeikasKülli SamuelTauno MetsaluHelle KarroAndrei SõritsaAndres SalumetsMaire PetersAccumulating data have shown the involvement of microRNAs (miRNAs) in endometriosis pathogenesis. In this study, we used a novel approach to determine the endometriotic lesion-specific miRNAs by high-throughput small RNA sequencing of paired samples of peritoneal endometriotic lesions and matched healthy surrounding tissues together with eutopic endometria of the same patients. We found five miRNAs specific to epithelial cells--miR-34c, miR-449a, miR-200a, miR-200b and miR-141 showing significantly higher expression in peritoneal endometriotic lesions compared to healthy peritoneal tissues. We also determined the expression levels of miR-200 family target genes E-cadherin, ZEB1 and ZEB2 and found that the expression level of E-cadherin was significantly higher in endometriotic lesions compared to healthy tissues. Further evaluation verified that studied miRNAs could be used as diagnostic markers for confirming the presence of endometrial cells in endometriotic lesion biopsy samples. Furthermore, we demonstrated that the miRNA profile of peritoneal endometriotic lesion biopsies is largely masked by the surrounding peritoneal tissue, challenging the discovery of an accurate lesion-specific miRNA profile. Taken together, our findings indicate that only particular miRNAs with a significantly higher expression in endometriotic cells can be detected from lesion biopsies, and can serve as diagnostic markers for endometriosis.http://europepmc.org/articles/PMC4227690?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Merli Saare Kadri Rekker Triin Laisk-Podar Deniss Sõritsa Anne Mari Roost Jaak Simm Agne Velthut-Meikas Külli Samuel Tauno Metsalu Helle Karro Andrei Sõritsa Andres Salumets Maire Peters |
spellingShingle |
Merli Saare Kadri Rekker Triin Laisk-Podar Deniss Sõritsa Anne Mari Roost Jaak Simm Agne Velthut-Meikas Külli Samuel Tauno Metsalu Helle Karro Andrei Sõritsa Andres Salumets Maire Peters High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues. PLoS ONE |
author_facet |
Merli Saare Kadri Rekker Triin Laisk-Podar Deniss Sõritsa Anne Mari Roost Jaak Simm Agne Velthut-Meikas Külli Samuel Tauno Metsalu Helle Karro Andrei Sõritsa Andres Salumets Maire Peters |
author_sort |
Merli Saare |
title |
High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues. |
title_short |
High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues. |
title_full |
High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues. |
title_fullStr |
High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues. |
title_full_unstemmed |
High-throughput sequencing approach uncovers the miRNome of peritoneal endometriotic lesions and adjacent healthy tissues. |
title_sort |
high-throughput sequencing approach uncovers the mirnome of peritoneal endometriotic lesions and adjacent healthy tissues. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Accumulating data have shown the involvement of microRNAs (miRNAs) in endometriosis pathogenesis. In this study, we used a novel approach to determine the endometriotic lesion-specific miRNAs by high-throughput small RNA sequencing of paired samples of peritoneal endometriotic lesions and matched healthy surrounding tissues together with eutopic endometria of the same patients. We found five miRNAs specific to epithelial cells--miR-34c, miR-449a, miR-200a, miR-200b and miR-141 showing significantly higher expression in peritoneal endometriotic lesions compared to healthy peritoneal tissues. We also determined the expression levels of miR-200 family target genes E-cadherin, ZEB1 and ZEB2 and found that the expression level of E-cadherin was significantly higher in endometriotic lesions compared to healthy tissues. Further evaluation verified that studied miRNAs could be used as diagnostic markers for confirming the presence of endometrial cells in endometriotic lesion biopsy samples. Furthermore, we demonstrated that the miRNA profile of peritoneal endometriotic lesion biopsies is largely masked by the surrounding peritoneal tissue, challenging the discovery of an accurate lesion-specific miRNA profile. Taken together, our findings indicate that only particular miRNAs with a significantly higher expression in endometriotic cells can be detected from lesion biopsies, and can serve as diagnostic markers for endometriosis. |
url |
http://europepmc.org/articles/PMC4227690?pdf=render |
work_keys_str_mv |
AT merlisaare highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT kadrirekker highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT triinlaiskpodar highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT denisssoritsa highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT annemariroost highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT jaaksimm highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT agnevelthutmeikas highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT kullisamuel highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT taunometsalu highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT hellekarro highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT andreisoritsa highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT andressalumets highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues AT mairepeters highthroughputsequencingapproachuncoversthemirnomeofperitonealendometrioticlesionsandadjacenthealthytissues |
_version_ |
1725934367072583680 |