Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates
INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducibl...
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doaj-494de56c10c94fb092b50632c587c5f02020-11-25T03:10:20ZengElsevierBrazilian Journal of Infectious Diseases1678-439114656456810.1590/S1413-86702010000600004S1413-86702010000600004Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolatesVivian de Lima Spode Coutinho0Rodrigo Minuto Paiva1Keli Cristine Reiter2Fernanda de-Paris3Afonso Luis Barth4Alice Beatriz Mombach Pinheiro Machado5Universidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulINTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. RESULTS: A total of 46.7% of staphylococci were positive for cMLS B; 3.3% for iMLS B and 3.3% for MS B. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. CONCLUSION: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004&lng=en&tlng=enStaphylococcusresistanceerm genesmacrolides |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vivian de Lima Spode Coutinho Rodrigo Minuto Paiva Keli Cristine Reiter Fernanda de-Paris Afonso Luis Barth Alice Beatriz Mombach Pinheiro Machado |
spellingShingle |
Vivian de Lima Spode Coutinho Rodrigo Minuto Paiva Keli Cristine Reiter Fernanda de-Paris Afonso Luis Barth Alice Beatriz Mombach Pinheiro Machado Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates Brazilian Journal of Infectious Diseases Staphylococcus resistance erm genes macrolides |
author_facet |
Vivian de Lima Spode Coutinho Rodrigo Minuto Paiva Keli Cristine Reiter Fernanda de-Paris Afonso Luis Barth Alice Beatriz Mombach Pinheiro Machado |
author_sort |
Vivian de Lima Spode Coutinho |
title |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_short |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_full |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_fullStr |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_full_unstemmed |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_sort |
distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1678-4391 |
description |
INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. RESULTS: A total of 46.7% of staphylococci were positive for cMLS B; 3.3% for iMLS B and 3.3% for MS B. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. CONCLUSION: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes. |
topic |
Staphylococcus resistance erm genes macrolides |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004&lng=en&tlng=en |
work_keys_str_mv |
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