Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine
Aim: The aim was to study the bronchoalveolar lavage (BAL) technique in evaluating the local immune response of pig immunized with Pasteurella multocida bacterin vaccine. Materials and Methods: Weaned piglets were immunized with formalin-inactivated P52 strain of P. multocida bacterin and evaluated...
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Veterinary World
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doaj-4959f17692724d3d96eb96a790b6cdd52021-08-02T14:55:33ZengVeterinary WorldVeterinary World0972-89882231-09162015-04-018443844210.14202/vetworld.2015.438-442Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccineShiney George0Nagendra Nath Barman1Anjan Jyoti Nath 2Bhupen Sarma3North Eastern Regional Disease Diagnostic Laboratory, Government of Assam, Khanapara, Guwahati - 781 022, Assam, India; shineygeorge0@gmail.comDepartment of Microbiology, College of Veterinary Science, Khanapara, Guwahati - 781 022, Assam, India; nnbarman@gmail.comPasteur Institute of India, Coonoor - 643 013, Nilgiris, Tamil Nadu, India; drnath76@gmail.comDepartment of Surgery and Radiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India; bhupensarma59@gmail.comAim: The aim was to study the bronchoalveolar lavage (BAL) technique in evaluating the local immune response of pig immunized with Pasteurella multocida bacterin vaccine. Materials and Methods: Weaned piglets were immunized with formalin-inactivated P52 strain of P. multocida bacterin and evaluated for pulmonary immune response in BAL fluid. BAL was performed before vaccination and at different post vaccination days. The BAL fluid was assayed using enzyme-linked immunosorbent assay to study the development of P. multocida specific antibody isotypes and also evaluated for different cell populations using standard protocol. Results: The average recovery percentage of BAL fluid varies from 58.33 to 61.33 in vaccinated and control group of piglets. The BAL fluid of vaccinated pigs showed increase in antibody titer up to 60th days post vaccination (8.98±0.33), IgG being the predominant isotype reached maximum titer of 6.12±0.20 on 45th days post vaccination, followed by IgM and a meager concentration of IgA could be detected. An increased concentration of the lymphocyte population and induction of plasma cells was detected in the BAL fluid of vaccinated pigs. Conclusion: Though intranasal vaccination with P. multocida plain bacterin vaccine could not provoke a strong immune response, but is promising as lymphocyte population was increased and plasma cells were detected. BAL can be performed repeatedly up to 3/4 months of age in pigs to study pulmonary immune response without affecting their health.http://www.veterinaryworld.org/Vol.8/April-2015/1.pdfbronchoalveolar lavagecell typesisotype specific enzyme linked immunosorbent assayP. multocidapigpulmonary immune response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shiney George Nagendra Nath Barman Anjan Jyoti Nath Bhupen Sarma |
spellingShingle |
Shiney George Nagendra Nath Barman Anjan Jyoti Nath Bhupen Sarma Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine Veterinary World bronchoalveolar lavage cell types isotype specific enzyme linked immunosorbent assay P. multocida pig pulmonary immune response |
author_facet |
Shiney George Nagendra Nath Barman Anjan Jyoti Nath Bhupen Sarma |
author_sort |
Shiney George |
title |
Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine |
title_short |
Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine |
title_full |
Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine |
title_fullStr |
Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine |
title_full_unstemmed |
Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine |
title_sort |
bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with pasteurella multocida bacterin vaccine |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2015-04-01 |
description |
Aim: The aim was to study the bronchoalveolar lavage (BAL) technique in evaluating the local immune response of pig immunized with Pasteurella multocida bacterin vaccine.
Materials and Methods: Weaned piglets were immunized with formalin-inactivated P52 strain of P. multocida bacterin and evaluated for pulmonary immune response in BAL fluid. BAL was performed before vaccination and at different post vaccination days. The BAL fluid was assayed using enzyme-linked immunosorbent assay to study the development of P. multocida specific antibody isotypes and also evaluated for different cell populations using standard protocol.
Results: The average recovery percentage of BAL fluid varies from 58.33 to 61.33 in vaccinated and control group of piglets. The BAL fluid of vaccinated pigs showed increase in antibody titer up to 60th days post vaccination (8.98±0.33), IgG being the predominant isotype reached maximum titer of 6.12±0.20 on 45th days post vaccination, followed by IgM and a meager concentration of IgA could be detected. An increased concentration of the lymphocyte population and induction of plasma cells was detected in the BAL fluid of vaccinated pigs.
Conclusion: Though intranasal vaccination with P. multocida plain bacterin vaccine could not provoke a strong immune response, but is promising as lymphocyte population was increased and plasma cells were detected. BAL can be performed repeatedly up to 3/4 months of age in pigs to study pulmonary immune response without affecting their health. |
topic |
bronchoalveolar lavage cell types isotype specific enzyme linked immunosorbent assay P. multocida pig pulmonary immune response |
url |
http://www.veterinaryworld.org/Vol.8/April-2015/1.pdf |
work_keys_str_mv |
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1721230886135922688 |