FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum
Histone lysine acetylation is catalyzed by acetyltransferases (HATs), which is important in regulating gene expression and physiological function in eukaryotic cells. HATs can be classified into two main types: A- and B-type HATs. Recently, in Fusarium graminearum, it has been reported that A-type H...
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doaj-498b1172d51a405a88b04a70095529fb2021-06-08T04:42:01ZengElsevierJournal of Integrative Agriculture2095-31192020-07-0119718131824FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearumWu-yun LÜ0Nan YANG1Zhe XU2Han DAI3Shuai TANG4Zheng-yi WANG5Correspondence LÜ Wu-yun, Mobile: +86-15336512118; State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. ChinaCorrespondence WANG Zheng-yi, Mobile: +86-18067908283; State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. ChinaHistone lysine acetylation is catalyzed by acetyltransferases (HATs), which is important in regulating gene expression and physiological function in eukaryotic cells. HATs can be classified into two main types: A- and B-type HATs. Recently, in Fusarium graminearum, it has been reported that A-type HATs are involved in hyphal development, conidiation, sexual reproduction and virulence. However, the biological roles of B-type HATs are unknown. Here we report the identification and characterization of two B-type HATs (FgHat1 and FgHat2) in F. graminearum. Targeted deletion of FgHAT1 did not result in any detectable phenotypes. However, ΔFghat2 mutants were severely defective in vegetative growth, conidia production and morphogenesis, deoxynivalenol (DON) biosynthesis and virulence. Interestingly, deletion of FgHAT2 resulted in significantly increased sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). Furthermore, double deletion mutants (ΔFghat1ΔFghat2) displayed similar phenotypes to the ΔFghat2 mutants. Taken together, we conclude that FgHat2 but not FgHat1 plays essential roles in regulating morphogenesis, DNA damage repair, DON production and virulence in F. graminearum.http://www.sciencedirect.com/science/article/pii/S2095311919628194Fusarium graminearumacetyltransferasesFgHAT2pathogenicity |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wu-yun LÜ Nan YANG Zhe XU Han DAI Shuai TANG Zheng-yi WANG |
spellingShingle |
Wu-yun LÜ Nan YANG Zhe XU Han DAI Shuai TANG Zheng-yi WANG FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum Journal of Integrative Agriculture Fusarium graminearum acetyltransferases FgHAT2 pathogenicity |
author_facet |
Wu-yun LÜ Nan YANG Zhe XU Han DAI Shuai TANG Zheng-yi WANG |
author_sort |
Wu-yun LÜ |
title |
FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum |
title_short |
FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum |
title_full |
FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum |
title_fullStr |
FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum |
title_full_unstemmed |
FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum |
title_sort |
fghat2 is involved in regulating vegetative growth, conidiation, dna damage repair, don production and virulence in fusarium graminearum |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2020-07-01 |
description |
Histone lysine acetylation is catalyzed by acetyltransferases (HATs), which is important in regulating gene expression and physiological function in eukaryotic cells. HATs can be classified into two main types: A- and B-type HATs. Recently, in Fusarium graminearum, it has been reported that A-type HATs are involved in hyphal development, conidiation, sexual reproduction and virulence. However, the biological roles of B-type HATs are unknown. Here we report the identification and characterization of two B-type HATs (FgHat1 and FgHat2) in F. graminearum. Targeted deletion of FgHAT1 did not result in any detectable phenotypes. However, ΔFghat2 mutants were severely defective in vegetative growth, conidia production and morphogenesis, deoxynivalenol (DON) biosynthesis and virulence. Interestingly, deletion of FgHAT2 resulted in significantly increased sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). Furthermore, double deletion mutants (ΔFghat1ΔFghat2) displayed similar phenotypes to the ΔFghat2 mutants. Taken together, we conclude that FgHat2 but not FgHat1 plays essential roles in regulating morphogenesis, DNA damage repair, DON production and virulence in F. graminearum. |
topic |
Fusarium graminearum acetyltransferases FgHAT2 pathogenicity |
url |
http://www.sciencedirect.com/science/article/pii/S2095311919628194 |
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