Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis
Abstract Background To investigate the heterogeneities of human umbilical cord mesenchymal stromal cells (HUCMSCs) derived from different donors and their therapeutic variations when applied to mouse liver fibrosis model. Methods The characteristics of HUCMSCs derived from multiple donors were compr...
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doaj-49945133604b40da8524c6fa2c6a8ad82021-06-27T11:08:45ZengBMCStem Cell Research & Therapy1757-65122021-06-0112111710.1186/s13287-021-02430-6Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosisYuanyuan Xie0Shuo Liu1Liudi Wang2Hui Yang3Chenxu Tai4Li Ling5Libo Chen6Shanshan Liu7Bin Wang8Clinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolClinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolClinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolClinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolClinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolDepartment of Endocrinology, University Health Science Center, Hua Zhong University of Science and Technology Union Shenzhen Hospital and The 6th Affiliated Hospital of ShenzhenDepartment of Endocrinology, University Health Science Center, Hua Zhong University of Science and Technology Union Shenzhen Hospital and The 6th Affiliated Hospital of ShenzhenDepartment of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolClinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolAbstract Background To investigate the heterogeneities of human umbilical cord mesenchymal stromal cells (HUCMSCs) derived from different donors and their therapeutic variations when applied to mouse liver fibrosis model. Methods The characteristics of HUCMSCs derived from multiple donors were comprehensively analyzed including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. Then, the HUCMSCs with distinct immunomodulatory effects were applied to treat mouse liver fibrosis and their therapeutic effects were observed. Results The HUCMSCs derived from multiple donors kept a high consistency in surface marker expressions, viability, growth curve, and tumorigenicity in nude mice but had robust heterogeneities in differentiation potentials and immune regulations. In addition, three HUCMSC lines applied to mice liver fibrosis model had different therapeutic outcomes, in line with individual immune regulation capability. Conclusion The HUCMSCs derived from different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stromal cell therapy.https://doi.org/10.1186/s13287-021-02430-6Cell therapyUmbilical cord mesenchymal stromal cellsHeterogeneityDifferentiationImmunomodulationLiver fibrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuanyuan Xie Shuo Liu Liudi Wang Hui Yang Chenxu Tai Li Ling Libo Chen Shanshan Liu Bin Wang |
spellingShingle |
Yuanyuan Xie Shuo Liu Liudi Wang Hui Yang Chenxu Tai Li Ling Libo Chen Shanshan Liu Bin Wang Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis Stem Cell Research & Therapy Cell therapy Umbilical cord mesenchymal stromal cells Heterogeneity Differentiation Immunomodulation Liver fibrosis |
author_facet |
Yuanyuan Xie Shuo Liu Liudi Wang Hui Yang Chenxu Tai Li Ling Libo Chen Shanshan Liu Bin Wang |
author_sort |
Yuanyuan Xie |
title |
Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis |
title_short |
Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis |
title_full |
Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis |
title_fullStr |
Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis |
title_full_unstemmed |
Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis |
title_sort |
individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high treg promotion demonstrate improved recovery of mouse liver fibrosis |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2021-06-01 |
description |
Abstract Background To investigate the heterogeneities of human umbilical cord mesenchymal stromal cells (HUCMSCs) derived from different donors and their therapeutic variations when applied to mouse liver fibrosis model. Methods The characteristics of HUCMSCs derived from multiple donors were comprehensively analyzed including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. Then, the HUCMSCs with distinct immunomodulatory effects were applied to treat mouse liver fibrosis and their therapeutic effects were observed. Results The HUCMSCs derived from multiple donors kept a high consistency in surface marker expressions, viability, growth curve, and tumorigenicity in nude mice but had robust heterogeneities in differentiation potentials and immune regulations. In addition, three HUCMSC lines applied to mice liver fibrosis model had different therapeutic outcomes, in line with individual immune regulation capability. Conclusion The HUCMSCs derived from different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stromal cell therapy. |
topic |
Cell therapy Umbilical cord mesenchymal stromal cells Heterogeneity Differentiation Immunomodulation Liver fibrosis |
url |
https://doi.org/10.1186/s13287-021-02430-6 |
work_keys_str_mv |
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1721358244388012032 |