Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro
The contribution of secretions from tumor-associated macrophage (TAM)-like cells to the stimulation of mechanical property changes in murine breast cancer cells was studied using an in vitro model system. A murine breast cancer cell line (FP10SC2) was stimulated by adding macrophage (J774.2) cultiva...
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doaj-49a83f081aca4ede82e34ce3ba3c83d42020-11-25T02:47:15ZengMDPI AGMicromachines2072-666X2019-10-01101173810.3390/mi10110738mi10110738Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in VitroHyonchol Kim0Kenta Ishibashi1Tomoko Okada2Chikashi Nakamura3Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanBiomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanBiomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanBiomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanThe contribution of secretions from tumor-associated macrophage (TAM)-like cells to the stimulation of mechanical property changes in murine breast cancer cells was studied using an in vitro model system. A murine breast cancer cell line (FP10SC2) was stimulated by adding macrophage (J774.2) cultivation medium containing stimulation molecules secreted from the macrophages, and changes in mechanical properties were compared before and after stimulation. As a result, cell elasticity decreased, degradation ability of the extracellular matrix increased, and the expression of plakoglobin was upregulated. These results indicate that cancer cell malignancy is upregulated by this stimulation. Moreover, changes in intercellular adhesion strengths between pairs of cancer cells were measured before and after stimulation using atomic force microscopy (AFM). The maximum force required to separate cells was increased by stimulation with the secreted factors. These results indicate the possibility that TAMs cause changes in the mechanical properties of cancer cells in tumor microenvironments, and in vitro measurements of mechanical property changes in cancer cells will be useful to study interactions between cells in tumor microenvironments.https://www.mdpi.com/2072-666X/10/11/738intercellular adhesion strengthbreast cancer cellmacrophagesecretionmechanical propertyatomic force microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyonchol Kim Kenta Ishibashi Tomoko Okada Chikashi Nakamura |
spellingShingle |
Hyonchol Kim Kenta Ishibashi Tomoko Okada Chikashi Nakamura Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro Micromachines intercellular adhesion strength breast cancer cell macrophage secretion mechanical property atomic force microscopy |
author_facet |
Hyonchol Kim Kenta Ishibashi Tomoko Okada Chikashi Nakamura |
author_sort |
Hyonchol Kim |
title |
Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro |
title_short |
Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro |
title_full |
Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro |
title_fullStr |
Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro |
title_full_unstemmed |
Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro |
title_sort |
mechanical property changes in breast cancer cells induced by stimulation with macrophage secretions in vitro |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2019-10-01 |
description |
The contribution of secretions from tumor-associated macrophage (TAM)-like cells to the stimulation of mechanical property changes in murine breast cancer cells was studied using an in vitro model system. A murine breast cancer cell line (FP10SC2) was stimulated by adding macrophage (J774.2) cultivation medium containing stimulation molecules secreted from the macrophages, and changes in mechanical properties were compared before and after stimulation. As a result, cell elasticity decreased, degradation ability of the extracellular matrix increased, and the expression of plakoglobin was upregulated. These results indicate that cancer cell malignancy is upregulated by this stimulation. Moreover, changes in intercellular adhesion strengths between pairs of cancer cells were measured before and after stimulation using atomic force microscopy (AFM). The maximum force required to separate cells was increased by stimulation with the secreted factors. These results indicate the possibility that TAMs cause changes in the mechanical properties of cancer cells in tumor microenvironments, and in vitro measurements of mechanical property changes in cancer cells will be useful to study interactions between cells in tumor microenvironments. |
topic |
intercellular adhesion strength breast cancer cell macrophage secretion mechanical property atomic force microscopy |
url |
https://www.mdpi.com/2072-666X/10/11/738 |
work_keys_str_mv |
AT hyoncholkim mechanicalpropertychangesinbreastcancercellsinducedbystimulationwithmacrophagesecretionsinvitro AT kentaishibashi mechanicalpropertychangesinbreastcancercellsinducedbystimulationwithmacrophagesecretionsinvitro AT tomokookada mechanicalpropertychangesinbreastcancercellsinducedbystimulationwithmacrophagesecretionsinvitro AT chikashinakamura mechanicalpropertychangesinbreastcancercellsinducedbystimulationwithmacrophagesecretionsinvitro |
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