Investigation the effect of olive oil feeding strategies on Yarrowia lipolytica lipase production

• Main Authors: Farshad Darvishi, Barumand Hosseini
• Format: Article
• Language: English
• Published: University of Isfahan 2015-12-01
• Series: Biological Journal of Microorganism
• Subjects: Lipase; Yarrowia lipolytica; Batch culture; Fed-batch culture; Feeding
• Online Access: http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-71-3&slc_lang=en&sid=1

Introduction: Lipase of the yeast Yarrowia lipolytica used in detergents, cosmetics, pharmaceuticals and food industries. This enzyme production depends on medium composition, especially carbon source.  The purpose of this study was to investigate the effect of olive oil different feeding strategies on Y. lipolytica lipase production. Materials and methods: The yeast strains Y. lipolytica FDY1390 was cultured in media with different feeding strategies. Three different models were used to fed-batch feeding method. The yeast growth is monitored by direct counting method with neubauer chamber. Lipase activity was measured using titration method. Results: All three models of fed-batch feeding increased lipase toward the batch culture. Fed3 was the best model of fed-batch feeding, in which the model leads to the production of lipase activity with 526 U/ml after 120 hours and the productivity reached to 4.38 U/ h. Discussion and conclusion: The results showed that feeding patterns of fed-batch culture increase production rate of lipase production towards batch culture. The best strategy is to add olive oil as the carbon source induction substrate in medium after 48 hours of inoculation.