Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates
Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2016-06-01
|
Series: | Brazilian Journal of Microbiology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381&lng=en&tlng=en |
id |
doaj-49be04db3a8949788e83ca67e6070865 |
---|---|
record_format |
Article |
spelling |
doaj-49be04db3a8949788e83ca67e60708652020-11-24T22:40:11ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052016-06-0147238138810.1016/j.bjm.2016.01.007S1517-83822016000200381Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolatesYasmine Fathy MohamedHamida Moustafa Abou-ShleibAmal Mohamed KhalilNadia Mohamed El-GuinkMoustafa Ahmed El-NakeebAbstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was also elucidated. Colistin was proved to be rapid bactericidal against pan-drug resistant isolates. It interacts with the outer bacterial membrane leading to deformation of its outline, pore formation, leakage of internal contents, cell lysis and finally death. Furthermore, variations in membrane composition of eukaryotic and microbial cells provide a key for colistin selectivity toward bacterial cells. Colistin selectively alters membrane permeability of pan-drug resistant isolates which leads to cell lysis. Colistin was proved to be an efficient last line treatment for pan-drug resistant infections which are hard to treat.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381&lng=en&tlng=enPan-drug resistantLeakagePermeability alterationColistinUltrastructure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yasmine Fathy Mohamed Hamida Moustafa Abou-Shleib Amal Mohamed Khalil Nadia Mohamed El-Guink Moustafa Ahmed El-Nakeeb |
spellingShingle |
Yasmine Fathy Mohamed Hamida Moustafa Abou-Shleib Amal Mohamed Khalil Nadia Mohamed El-Guink Moustafa Ahmed El-Nakeeb Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates Brazilian Journal of Microbiology Pan-drug resistant Leakage Permeability alteration Colistin Ultrastructure |
author_facet |
Yasmine Fathy Mohamed Hamida Moustafa Abou-Shleib Amal Mohamed Khalil Nadia Mohamed El-Guink Moustafa Ahmed El-Nakeeb |
author_sort |
Yasmine Fathy Mohamed |
title |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_short |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_full |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_fullStr |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_full_unstemmed |
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates |
title_sort |
membrane permeabilization of colistin toward pan-drug resistant gram-negative isolates |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2016-06-01 |
description |
Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was also elucidated. Colistin was proved to be rapid bactericidal against pan-drug resistant isolates. It interacts with the outer bacterial membrane leading to deformation of its outline, pore formation, leakage of internal contents, cell lysis and finally death. Furthermore, variations in membrane composition of eukaryotic and microbial cells provide a key for colistin selectivity toward bacterial cells. Colistin selectively alters membrane permeability of pan-drug resistant isolates which leads to cell lysis. Colistin was proved to be an efficient last line treatment for pan-drug resistant infections which are hard to treat. |
topic |
Pan-drug resistant Leakage Permeability alteration Colistin Ultrastructure |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381&lng=en&tlng=en |
work_keys_str_mv |
AT yasminefathymohamed membranepermeabilizationofcolistintowardpandrugresistantgramnegativeisolates AT hamidamoustafaaboushleib membranepermeabilizationofcolistintowardpandrugresistantgramnegativeisolates AT amalmohamedkhalil membranepermeabilizationofcolistintowardpandrugresistantgramnegativeisolates AT nadiamohamedelguink membranepermeabilizationofcolistintowardpandrugresistantgramnegativeisolates AT moustafaahmedelnakeeb membranepermeabilizationofcolistintowardpandrugresistantgramnegativeisolates |
_version_ |
1725705584372613120 |