Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms
Escherichia coli-associated urinary tract infections (UTIs) are among the most common bacterial infections in humans. UTIs are usually managed with antibiotic therapy, but over the years, antibiotic-resistant strains of uropathogenic E. coli (UPEC) have emerged. The formation of biofilms further com...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-04-01
|
Series: | Viruses |
Subjects: | |
Online Access: | http://www.mdpi.com/1999-4915/4/4/471/ |
id |
doaj-49cf6672ed764b8ea28bc9107b108888 |
---|---|
record_format |
Article |
spelling |
doaj-49cf6672ed764b8ea28bc9107b1088882020-11-24T22:05:42ZengMDPI AGViruses1999-49152012-04-014447148710.3390/v4040471Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli BiofilmsAmee MangesJosée HarelHans-W. AckermannAndrew M. KropinskiPatrick BoerlinAndrew ChibeuErika J. LingohrLuke MassonEscherichia coli-associated urinary tract infections (UTIs) are among the most common bacterial infections in humans. UTIs are usually managed with antibiotic therapy, but over the years, antibiotic-resistant strains of uropathogenic E. coli (UPEC) have emerged. The formation of biofilms further complicates the treatment of these infections by making them resistant to killing by the host immune system as well as by antibiotics. This has encouraged research into therapy using bacteriophages (phages) as a supplement or substitute for antibiotics. In this study we characterized 253 UPEC in terms of their biofilm-forming capabilities, serotype, and antimicrobial resistance. Three phages were then isolated (vB_EcoP_ACG-C91, vB_EcoM_ACG-C40 and vB_EcoS_ACG-M12) which were able to lyse 80.5% of a subset (42) of the UPEC strains able to form biofilms. Correlation was established between phage sensitivity and specific serotypes of the UPEC strains. The phages’ genome sequences were determined and resulted in classification of vB_EcoP_ACG-C91 as a SP6likevirus, vB_EcoM_ACG-C40 as a T4likevirus and vB_EcoS_ACG-M12 as T1likevirus. We assessed the ability of the three phages to eradicate the established biofilm of one of the UPEC strains used in the study. All phages significantly reduced the biofilm within 2–12 h of incubation.http://www.mdpi.com/1999-4915/4/4/471/UPECbacteriophagebiofilms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amee Manges Josée Harel Hans-W. Ackermann Andrew M. Kropinski Patrick Boerlin Andrew Chibeu Erika J. Lingohr Luke Masson |
spellingShingle |
Amee Manges Josée Harel Hans-W. Ackermann Andrew M. Kropinski Patrick Boerlin Andrew Chibeu Erika J. Lingohr Luke Masson Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms Viruses UPEC bacteriophage biofilms |
author_facet |
Amee Manges Josée Harel Hans-W. Ackermann Andrew M. Kropinski Patrick Boerlin Andrew Chibeu Erika J. Lingohr Luke Masson |
author_sort |
Amee Manges |
title |
Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms |
title_short |
Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms |
title_full |
Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms |
title_fullStr |
Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms |
title_full_unstemmed |
Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms |
title_sort |
bacteriophages with the ability to degrade uropathogenic escherichia coli biofilms |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2012-04-01 |
description |
Escherichia coli-associated urinary tract infections (UTIs) are among the most common bacterial infections in humans. UTIs are usually managed with antibiotic therapy, but over the years, antibiotic-resistant strains of uropathogenic E. coli (UPEC) have emerged. The formation of biofilms further complicates the treatment of these infections by making them resistant to killing by the host immune system as well as by antibiotics. This has encouraged research into therapy using bacteriophages (phages) as a supplement or substitute for antibiotics. In this study we characterized 253 UPEC in terms of their biofilm-forming capabilities, serotype, and antimicrobial resistance. Three phages were then isolated (vB_EcoP_ACG-C91, vB_EcoM_ACG-C40 and vB_EcoS_ACG-M12) which were able to lyse 80.5% of a subset (42) of the UPEC strains able to form biofilms. Correlation was established between phage sensitivity and specific serotypes of the UPEC strains. The phages’ genome sequences were determined and resulted in classification of vB_EcoP_ACG-C91 as a SP6likevirus, vB_EcoM_ACG-C40 as a T4likevirus and vB_EcoS_ACG-M12 as T1likevirus. We assessed the ability of the three phages to eradicate the established biofilm of one of the UPEC strains used in the study. All phages significantly reduced the biofilm within 2–12 h of incubation. |
topic |
UPEC bacteriophage biofilms |
url |
http://www.mdpi.com/1999-4915/4/4/471/ |
work_keys_str_mv |
AT ameemanges bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT joseeharel bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT hanswackermann bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT andrewmkropinski bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT patrickboerlin bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT andrewchibeu bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT erikajlingohr bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms AT lukemasson bacteriophageswiththeabilitytodegradeuropathogenicescherichiacolibiofilms |
_version_ |
1725825059435577344 |