Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast.
Addition of glucose to starved yeast cells elicits a dramatic restructuring of the transcriptional and metabolic state of the cell. While many components of the signaling network responsible for this response have been identified, a comprehensive view of this network is lacking. We have used global...
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doaj-49e8090533924ee688e6dee1d036aaa92021-07-02T13:59:40ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852004-05-0125E12810.1371/journal.pbio.0020128Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast.Ying WangMichael PierceLisa SchneperC Gökçe GüldalXiuying ZhangSaeed TavazoieJames R BroachAddition of glucose to starved yeast cells elicits a dramatic restructuring of the transcriptional and metabolic state of the cell. While many components of the signaling network responsible for this response have been identified, a comprehensive view of this network is lacking. We have used global analysis of gene expression to assess the roles of the small GTP-binding proteins, Ras2 and Gpa2, in mediating the transcriptional response to glucose. We find that 90% of the transcriptional changes in the cell attendant on glucose addition are recapitulated by activation of Ras2 or Gpa2. In addition, we find that protein kinase A (PKA) mediates all of the Ras2 and Gpa2 transcriptional effects. However, we also find that most of the transcriptional effects of glucose addition to wild-type cells are retained in strains containing a PKA unresponsive to changes in cAMP levels. Thus, most glucose-responsive genes are regulated redundantly by a Ras/PKA-dependent pathway and by one or more PKA-independent pathways. Computational analysis extracted RRPE/PAC as the major response element for Ras and glucose regulation and revealed additional response elements mediating glucose and Ras regulation. These studies provide a paradigm for extracting the topology of signal transduction pathways from expression data.http://europepmc.org/articles/PMC406390?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Wang Michael Pierce Lisa Schneper C Gökçe Güldal Xiuying Zhang Saeed Tavazoie James R Broach |
spellingShingle |
Ying Wang Michael Pierce Lisa Schneper C Gökçe Güldal Xiuying Zhang Saeed Tavazoie James R Broach Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. PLoS Biology |
author_facet |
Ying Wang Michael Pierce Lisa Schneper C Gökçe Güldal Xiuying Zhang Saeed Tavazoie James R Broach |
author_sort |
Ying Wang |
title |
Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. |
title_short |
Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. |
title_full |
Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. |
title_fullStr |
Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. |
title_full_unstemmed |
Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. |
title_sort |
ras and gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2004-05-01 |
description |
Addition of glucose to starved yeast cells elicits a dramatic restructuring of the transcriptional and metabolic state of the cell. While many components of the signaling network responsible for this response have been identified, a comprehensive view of this network is lacking. We have used global analysis of gene expression to assess the roles of the small GTP-binding proteins, Ras2 and Gpa2, in mediating the transcriptional response to glucose. We find that 90% of the transcriptional changes in the cell attendant on glucose addition are recapitulated by activation of Ras2 or Gpa2. In addition, we find that protein kinase A (PKA) mediates all of the Ras2 and Gpa2 transcriptional effects. However, we also find that most of the transcriptional effects of glucose addition to wild-type cells are retained in strains containing a PKA unresponsive to changes in cAMP levels. Thus, most glucose-responsive genes are regulated redundantly by a Ras/PKA-dependent pathway and by one or more PKA-independent pathways. Computational analysis extracted RRPE/PAC as the major response element for Ras and glucose regulation and revealed additional response elements mediating glucose and Ras regulation. These studies provide a paradigm for extracting the topology of signal transduction pathways from expression data. |
url |
http://europepmc.org/articles/PMC406390?pdf=render |
work_keys_str_mv |
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