Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran
Contagious agalactiae (C.A.) is one of the most common disease affecting small ruminants which is caused by Mycoplasma agalactiae. This disease is particularly widespread around the world and Iran is one of the countries that C.A. is present. The aim of this study was isolation and identification of...
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Razi Vaccine and Serum Research Institute
2011-06-01
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doaj-49f12c21fe764a4a848bd60d06bd6bf22020-11-24T20:58:27Zeng Razi Vaccine and Serum Research InstituteArchives of Razi Institute 0365-34392008-98722011-06-016611116Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, IranKhaki, P.Moradi Bidhendi, S.Pilehchian Langroudi, R.Moradi Bidhendi, S.Khaki, P.Pilehchian Langroudi, R.Contagious agalactiae (C.A.) is one of the most common disease affecting small ruminants which is caused by Mycoplasma agalactiae. This disease is particularly widespread around the world and Iran is one of the countries that C.A. is present. The aim of this study was isolation and identification of M. agalactiae (MG) with culture and PCR technique in milk samples in Kordestan province, Iran. A total of 367 milk samples were collected from sheep and goat. Specific published primers amplify a 375 bp gene of MG were used for PCR. Twenty (5.5%) out of 367 were positive in PPLO agar and 5 (25%) out of these isolates were positive with Mycoplasma agalactiae primers. Four (75%) out of 5 isolates was from sheep and 1(25%) from goat. Result of PCR with 367 milk samples showed that 11(3%) of them were positive with these primers. The isolation of M. agalactiae showed that C.A is present in Kordestan province and our results suggested that PCR method because of reduces the time consuming could be an alternative method beside culture.http://www.archrazi.com/browse.php?a_id=201&sid=1&slc_lang=enMycoplasma agalactiaeCulturePCRSheepGoat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khaki, P. Moradi Bidhendi, S. Pilehchian Langroudi, R. Moradi Bidhendi, S. Khaki, P. Pilehchian Langroudi, R. |
spellingShingle |
Khaki, P. Moradi Bidhendi, S. Pilehchian Langroudi, R. Moradi Bidhendi, S. Khaki, P. Pilehchian Langroudi, R. Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran Archives of Razi Institute Mycoplasma agalactiae Culture PCR Sheep Goat |
author_facet |
Khaki, P. Moradi Bidhendi, S. Pilehchian Langroudi, R. Moradi Bidhendi, S. Khaki, P. Pilehchian Langroudi, R. |
author_sort |
Khaki, P. |
title |
Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran |
title_short |
Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran |
title_full |
Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran |
title_fullStr |
Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran |
title_full_unstemmed |
Isolation and identification of Mycoplasma agalactiae by culture and Polymerase Chain Reaction in Sheep and Goat Milk Samples in Kordestan province, Iran |
title_sort |
isolation and identification of mycoplasma agalactiae by culture and polymerase chain reaction in sheep and goat milk samples in kordestan province, iran |
publisher |
Razi Vaccine and Serum Research Institute |
series |
Archives of Razi Institute |
issn |
0365-3439 2008-9872 |
publishDate |
2011-06-01 |
description |
Contagious agalactiae (C.A.) is one of the most common disease affecting small ruminants which is caused by Mycoplasma agalactiae. This disease is particularly widespread around the world and Iran is one of the countries that C.A. is present. The aim of this study was isolation and identification of M. agalactiae (MG) with culture and PCR technique in milk samples in Kordestan province, Iran. A total of 367 milk samples were collected from sheep and goat. Specific published primers amplify a 375 bp gene of MG were used for PCR. Twenty (5.5%) out of 367 were positive in PPLO agar and 5 (25%) out of these isolates were positive with Mycoplasma agalactiae primers. Four (75%) out of 5 isolates was from sheep and 1(25%) from goat. Result of PCR with 367 milk samples showed that 11(3%) of them were positive with these primers. The isolation of M. agalactiae showed that C.A is present in Kordestan province and our results suggested that PCR method because of reduces the time consuming could be an alternative method beside culture. |
topic |
Mycoplasma agalactiae Culture PCR Sheep Goat |
url |
http://www.archrazi.com/browse.php?a_id=201&sid=1&slc_lang=en |
work_keys_str_mv |
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