| Summary: | Lifen Peng,1 Hailan Yan,2 Shuyi Qi,3 Lifei Deng4 1Department of Otolaryngology, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, Nanchang, People’s Republic of China; 2Department of Clinical Medicine, Xi’an Jiaotong University Health Science Center, Xi’an, People’s Republic of China; 3Department of Pathology, Jiangxi Provincial Cancer Hospital, Nanchang, People’s Republic of China; 4Head and Neck Cancer Surgery, Jiangxi Provincial Cancer Hospital, Nanchang, People’s Republic of ChinaCorrespondence: Lifei DengHead and Neck Cancer Surgery, Jiangxi Provincial Cancer Hospital, Nanchang 330006, People’s Republic of ChinaTel +86 13657099876Email denglifei@126.comAim: To investigate the role of CAB39 in nasopharyngeal carcinoma (NPC) development and examine its expression level in NPC tumor samples.Methods: Immunohistochemistry staining of NPC tissue microarray was conducted to detect the expression of CAB39 protein in NPC tissues, and the clinical significance of CAB39 was evaluated. Lentivirus-mediated over-expression of CAB39 was designed to increase CAB39 expression in CNE-1 cells. Cell colony formation, cell cycle and CCK-8 proliferation experiments were performed to compare the proliferation ability of CNE-1 cells with or without CAB39 over-expression. Western blotting was conducted to examine downstream targets of CAB39.Results: CAB39 expression was higher in tumor samples compared to normal tissue and the higher CAB39 expression was positively correlated to higher TNM stage and distant metastasis rate and non-keratinized state. Further, CAB39 over-expression dramatically increased the proliferation and colony formation of CNE-1 cells. Finally, higher p-JNK protein level was found in CAB39 over-expressing cells.Conclusion: CAB39 promotes the proliferation of CNE-1 cells via up-regulating p-JNK.Keywords: CAB39, proliferation, nasopharyngeal carcinoma, p-JNK
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