Isolation and Characterization of Chelonia mydas Myoglobin
Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To ac...
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Bogor Agricultural University
2012-06-01
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doaj-4a079d044709455797377bcaa2cce7e22020-11-25T00:53:54ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942012-06-011926064Isolation and Characterization of Chelonia mydas MyoglobinMOHAMAD SADIKINRINI PUSPITANINGRUMSEPTELIA INAWATI WANANDIRONDANG ROEMIATI SOEGIANTOGreen sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied icroscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by ephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins.http://journal.ipb.ac.id/index.php/hayati/article/view/5305/3701green sea turtlemyoglobinmolecular weightisolationcharacterization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
MOHAMAD SADIKIN RINI PUSPITANINGRUM SEPTELIA INAWATI WANANDI RONDANG ROEMIATI SOEGIANTO |
spellingShingle |
MOHAMAD SADIKIN RINI PUSPITANINGRUM SEPTELIA INAWATI WANANDI RONDANG ROEMIATI SOEGIANTO Isolation and Characterization of Chelonia mydas Myoglobin Hayati Journal of Biosciences green sea turtle myoglobin molecular weight isolation characterization |
author_facet |
MOHAMAD SADIKIN RINI PUSPITANINGRUM SEPTELIA INAWATI WANANDI RONDANG ROEMIATI SOEGIANTO |
author_sort |
MOHAMAD SADIKIN |
title |
Isolation and Characterization of Chelonia mydas Myoglobin |
title_short |
Isolation and Characterization of Chelonia mydas Myoglobin |
title_full |
Isolation and Characterization of Chelonia mydas Myoglobin |
title_fullStr |
Isolation and Characterization of Chelonia mydas Myoglobin |
title_full_unstemmed |
Isolation and Characterization of Chelonia mydas Myoglobin |
title_sort |
isolation and characterization of chelonia mydas myoglobin |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 2086-4094 |
publishDate |
2012-06-01 |
description |
Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied icroscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by ephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins. |
topic |
green sea turtle myoglobin molecular weight isolation characterization |
url |
http://journal.ipb.ac.id/index.php/hayati/article/view/5305/3701 |
work_keys_str_mv |
AT mohamadsadikin isolationandcharacterizationofcheloniamydasmyoglobin AT rinipuspitaningrum isolationandcharacterizationofcheloniamydasmyoglobin AT septeliainawatiwanandi isolationandcharacterizationofcheloniamydasmyoglobin AT rondangroemiatisoegianto isolationandcharacterizationofcheloniamydasmyoglobin |
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