Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies

For reconstruction of controlled cell division in a minimal cell model, or protocell, a positioning mechanism that spatially regulates division is indispensable. In Escherichia coli, the Min proteins oscillate from pole to pole to determine the division site by inhibition of the primary divisome pro...

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Main Authors:	Simon Kretschmer, Petra Schwille
Format:	Article
Language:	English
Published:	MDPI AG 2014-12-01
Series:	Life
Subjects:	protocell bottom-up synthetic biology cell division Min proteins membranes self-organization pattern formation
Online Access:	http://www.mdpi.com/2075-1729/4/4/915

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spelling	doaj-4a3f5f18e5d342efb805d274ff4778a12020-11-24T20:40:36ZengMDPI AGLife2075-17292014-12-014491592810.3390/life4040915life4040915Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro StudiesSimon Kretschmer0Petra Schwille1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, GermanyDepartment of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, GermanyFor reconstruction of controlled cell division in a minimal cell model, or protocell, a positioning mechanism that spatially regulates division is indispensable. In Escherichia coli, the Min proteins oscillate from pole to pole to determine the division site by inhibition of the primary divisome protein FtsZ anywhere but in the cell middle. Remarkably, when reconstituted under defined conditions in vitro, the Min proteins self-organize into spatiotemporal patterns in the presence of a lipid membrane and ATP. We review recent progress made in studying the Min system in vitro, particularly focusing on the effects of various physicochemical parameters and boundary conditions on pattern formation. Furthermore, we discuss implications and challenges for utilizing the Min system for division site placement in protocells.http://www.mdpi.com/2075-1729/4/4/915protocellbottom-up synthetic biologycell divisionMin proteinsmembranesself-organizationpattern formation
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Simon Kretschmer Petra Schwille
spellingShingle	Simon Kretschmer Petra Schwille Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies Life protocell bottom-up synthetic biology cell division Min proteins membranes self-organization pattern formation
author_facet	Simon Kretschmer Petra Schwille
author_sort	Simon Kretschmer
title	Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies
title_short	Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies
title_full	Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies
title_fullStr	Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies
title_full_unstemmed	Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies
title_sort	toward spatially regulated division of protocells: insights into the e. coli min system from in vitro studies
publisher	MDPI AG
series	Life
issn	2075-1729
publishDate	2014-12-01
description	For reconstruction of controlled cell division in a minimal cell model, or protocell, a positioning mechanism that spatially regulates division is indispensable. In Escherichia coli, the Min proteins oscillate from pole to pole to determine the division site by inhibition of the primary divisome protein FtsZ anywhere but in the cell middle. Remarkably, when reconstituted under defined conditions in vitro, the Min proteins self-organize into spatiotemporal patterns in the presence of a lipid membrane and ATP. We review recent progress made in studying the Min system in vitro, particularly focusing on the effects of various physicochemical parameters and boundary conditions on pattern formation. Furthermore, we discuss implications and challenges for utilizing the Min system for division site placement in protocells.
topic	protocell bottom-up synthetic biology cell division Min proteins membranes self-organization pattern formation
url	http://www.mdpi.com/2075-1729/4/4/915
work_keys_str_mv	AT simonkretschmer towardspatiallyregulateddivisionofprotocellsinsightsintotheecoliminsystemfrominvitrostudies AT petraschwille towardspatiallyregulateddivisionofprotocellsinsightsintotheecoliminsystemfrominvitrostudies
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Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies

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