Plasmodium vivax Diversity and Population Structure across Four Continents.
Plasmodium vivax is the geographically most widespread human malaria parasite. To analyze patterns of microsatellite diversity and population structure across countries of different transmission intensity, genotyping data from 11 microsatellite markers was either generated or compiled from 841 isola...
Main Authors: | , , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS Neglected Tropical Diseases |
Online Access: | http://europepmc.org/articles/PMC4488360?pdf=render |
id |
doaj-4a40326eeb934297b02a96d9d41c5c3b |
---|---|
record_format |
Article |
spelling |
doaj-4a40326eeb934297b02a96d9d41c5c3b2020-11-25T01:38:43ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352015-01-0196e000387210.1371/journal.pntd.0003872Plasmodium vivax Diversity and Population Structure across Four Continents.Cristian KoepfliPriscila T RodriguesTiago AntaoPamela Orjuela-SánchezPeter Van den EedeDionicia GamboaNguyen van HongJorge BendezuAnnette ErhartCéline BarnadasArsène RatsimbasoaDidier MenardCarlo SeveriniMichela MenegonBakri Y M NourNadira KarunaweeraIvo MuellerMarcelo U FerreiraIngrid FelgerPlasmodium vivax is the geographically most widespread human malaria parasite. To analyze patterns of microsatellite diversity and population structure across countries of different transmission intensity, genotyping data from 11 microsatellite markers was either generated or compiled from 841 isolates from four continents collected in 1999-2008. Diversity was highest in South-East Asia (mean allelic richness 10.0-12.8), intermediate in the South Pacific (8.1-9.9) Madagascar and Sudan (7.9-8.4), and lowest in South America and Central Asia (5.5-7.2). A reduced panel of only 3 markers was sufficient to identify approx. 90% of all haplotypes in South Pacific, African and SE-Asian populations, but only 60-80% in Latin American populations, suggesting that typing of 2-6 markers, depending on the level of endemicity, is sufficient for epidemiological studies. Clustering analysis showed distinct clusters in Peru and Brazil, but little sub-structuring was observed within Africa, SE-Asia or the South Pacific. Isolates from Uzbekistan were exceptional, as a near-clonal parasite population was observed that was clearly separated from all other populations (FST>0.2). Outside Central Asia FST values were highest (0.11-0.16) between South American and all other populations, and lowest (0.04-0.07) between populations from South-East Asia and the South Pacific. These comparisons between P. vivax populations from four continents indicated that not only transmission intensity, but also geographical isolation affect diversity and population structure. However, the high effective population size results in slow changes of these parameters. This persistency must be taken into account when assessing the impact of control programs on the genetic structure of parasite populations.http://europepmc.org/articles/PMC4488360?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristian Koepfli Priscila T Rodrigues Tiago Antao Pamela Orjuela-Sánchez Peter Van den Eede Dionicia Gamboa Nguyen van Hong Jorge Bendezu Annette Erhart Céline Barnadas Arsène Ratsimbasoa Didier Menard Carlo Severini Michela Menegon Bakri Y M Nour Nadira Karunaweera Ivo Mueller Marcelo U Ferreira Ingrid Felger |
spellingShingle |
Cristian Koepfli Priscila T Rodrigues Tiago Antao Pamela Orjuela-Sánchez Peter Van den Eede Dionicia Gamboa Nguyen van Hong Jorge Bendezu Annette Erhart Céline Barnadas Arsène Ratsimbasoa Didier Menard Carlo Severini Michela Menegon Bakri Y M Nour Nadira Karunaweera Ivo Mueller Marcelo U Ferreira Ingrid Felger Plasmodium vivax Diversity and Population Structure across Four Continents. PLoS Neglected Tropical Diseases |
author_facet |
Cristian Koepfli Priscila T Rodrigues Tiago Antao Pamela Orjuela-Sánchez Peter Van den Eede Dionicia Gamboa Nguyen van Hong Jorge Bendezu Annette Erhart Céline Barnadas Arsène Ratsimbasoa Didier Menard Carlo Severini Michela Menegon Bakri Y M Nour Nadira Karunaweera Ivo Mueller Marcelo U Ferreira Ingrid Felger |
author_sort |
Cristian Koepfli |
title |
Plasmodium vivax Diversity and Population Structure across Four Continents. |
title_short |
Plasmodium vivax Diversity and Population Structure across Four Continents. |
title_full |
Plasmodium vivax Diversity and Population Structure across Four Continents. |
title_fullStr |
Plasmodium vivax Diversity and Population Structure across Four Continents. |
title_full_unstemmed |
Plasmodium vivax Diversity and Population Structure across Four Continents. |
title_sort |
plasmodium vivax diversity and population structure across four continents. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2015-01-01 |
description |
Plasmodium vivax is the geographically most widespread human malaria parasite. To analyze patterns of microsatellite diversity and population structure across countries of different transmission intensity, genotyping data from 11 microsatellite markers was either generated or compiled from 841 isolates from four continents collected in 1999-2008. Diversity was highest in South-East Asia (mean allelic richness 10.0-12.8), intermediate in the South Pacific (8.1-9.9) Madagascar and Sudan (7.9-8.4), and lowest in South America and Central Asia (5.5-7.2). A reduced panel of only 3 markers was sufficient to identify approx. 90% of all haplotypes in South Pacific, African and SE-Asian populations, but only 60-80% in Latin American populations, suggesting that typing of 2-6 markers, depending on the level of endemicity, is sufficient for epidemiological studies. Clustering analysis showed distinct clusters in Peru and Brazil, but little sub-structuring was observed within Africa, SE-Asia or the South Pacific. Isolates from Uzbekistan were exceptional, as a near-clonal parasite population was observed that was clearly separated from all other populations (FST>0.2). Outside Central Asia FST values were highest (0.11-0.16) between South American and all other populations, and lowest (0.04-0.07) between populations from South-East Asia and the South Pacific. These comparisons between P. vivax populations from four continents indicated that not only transmission intensity, but also geographical isolation affect diversity and population structure. However, the high effective population size results in slow changes of these parameters. This persistency must be taken into account when assessing the impact of control programs on the genetic structure of parasite populations. |
url |
http://europepmc.org/articles/PMC4488360?pdf=render |
work_keys_str_mv |
AT cristiankoepfli plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT priscilatrodrigues plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT tiagoantao plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT pamelaorjuelasanchez plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT petervandeneede plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT dioniciagamboa plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT nguyenvanhong plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT jorgebendezu plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT annetteerhart plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT celinebarnadas plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT arseneratsimbasoa plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT didiermenard plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT carloseverini plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT michelamenegon plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT bakriymnour plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT nadirakarunaweera plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT ivomueller plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT marcelouferreira plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents AT ingridfelger plasmodiumvivaxdiversityandpopulationstructureacrossfourcontinents |
_version_ |
1725051937060028416 |