Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.

Dysregulation of miRNAs and their target genes contributes to the pathophysiology of autoimmune diseases. Circulating miRNAs may serve as diagnostic/prognostic biomarkers. We aimed to investigate the association between circulating miRNAs, disease activity and spinal involvement in patients with axi...

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Main Authors: Klára Prajzlerová, Kristýna Grobelná, Markéta Hušáková, Šárka Forejtová, Astrid Jüngel, Steffen Gay, Jiří Vencovský, Karel Pavelka, Ladislav Šenolt, Mária Filková
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5609864?pdf=render
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spelling doaj-4a4f7f3eaa8640f5a9078b2385023d002020-11-24T21:24:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018532310.1371/journal.pone.0185323Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.Klára PrajzlerováKristýna GrobelnáMarkéta HušákováŠárka ForejtováAstrid JüngelSteffen GayJiří VencovskýKarel PavelkaLadislav ŠenoltMária FilkováDysregulation of miRNAs and their target genes contributes to the pathophysiology of autoimmune diseases. Circulating miRNAs may serve as diagnostic/prognostic biomarkers. We aimed to investigate the association between circulating miRNAs, disease activity and spinal involvement in patients with axial spondyloarthritis (AxSpA).Total RNA was isolated from the plasma of patients with non-radiographic (nr)AxSpA, patients with ankylosing spondylitis (AS) and healthy controls (HC) via phenol-chloroform extraction. A total of 760 miRNAs were analysed with TaqMan® Low Density Arrays, and the expression of 21 miRNAs was assessed using single assays.Comprehensive analysis demonstrated the differential expression of miRNAs among patients with progressive spinal disease. Of the 21 miRNAs selected according to their expression patterns, the levels of miR-625-3p were significantly different between nr-AxSpA patients and HCs. We found no correlation between miRNA levels and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) in nr-AxSpA patients. Selected miRNAs, such as miR-29a-3p, miR-146a-5p or miR-222-3p with an established role in extracellular matrix formation and inflammation were associated with spinal changes and/or disease activity assessed by BASDAI in AS patients, including miR-625-3p reflecting disease activity in AS with spinal involvement.Our data indicate that circulating miRNAs play a role in the pathogenesis of AxSpA and are also suggestive of their potential as biomarkers of disease progression. We hypothesize that differential systemic levels of miRNA expression reflect miRNA dysregulation at sites of spinal inflammation or bone formation where these molecules contribute to the development of pathophysiological features typical of AxSpA.http://europepmc.org/articles/PMC5609864?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Klára Prajzlerová
Kristýna Grobelná
Markéta Hušáková
Šárka Forejtová
Astrid Jüngel
Steffen Gay
Jiří Vencovský
Karel Pavelka
Ladislav Šenolt
Mária Filková
spellingShingle Klára Prajzlerová
Kristýna Grobelná
Markéta Hušáková
Šárka Forejtová
Astrid Jüngel
Steffen Gay
Jiří Vencovský
Karel Pavelka
Ladislav Šenolt
Mária Filková
Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.
PLoS ONE
author_facet Klára Prajzlerová
Kristýna Grobelná
Markéta Hušáková
Šárka Forejtová
Astrid Jüngel
Steffen Gay
Jiří Vencovský
Karel Pavelka
Ladislav Šenolt
Mária Filková
author_sort Klára Prajzlerová
title Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.
title_short Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.
title_full Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.
title_fullStr Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.
title_full_unstemmed Association between circulating miRNAs and spinal involvement in patients with axial spondyloarthritis.
title_sort association between circulating mirnas and spinal involvement in patients with axial spondyloarthritis.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description Dysregulation of miRNAs and their target genes contributes to the pathophysiology of autoimmune diseases. Circulating miRNAs may serve as diagnostic/prognostic biomarkers. We aimed to investigate the association between circulating miRNAs, disease activity and spinal involvement in patients with axial spondyloarthritis (AxSpA).Total RNA was isolated from the plasma of patients with non-radiographic (nr)AxSpA, patients with ankylosing spondylitis (AS) and healthy controls (HC) via phenol-chloroform extraction. A total of 760 miRNAs were analysed with TaqMan® Low Density Arrays, and the expression of 21 miRNAs was assessed using single assays.Comprehensive analysis demonstrated the differential expression of miRNAs among patients with progressive spinal disease. Of the 21 miRNAs selected according to their expression patterns, the levels of miR-625-3p were significantly different between nr-AxSpA patients and HCs. We found no correlation between miRNA levels and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) in nr-AxSpA patients. Selected miRNAs, such as miR-29a-3p, miR-146a-5p or miR-222-3p with an established role in extracellular matrix formation and inflammation were associated with spinal changes and/or disease activity assessed by BASDAI in AS patients, including miR-625-3p reflecting disease activity in AS with spinal involvement.Our data indicate that circulating miRNAs play a role in the pathogenesis of AxSpA and are also suggestive of their potential as biomarkers of disease progression. We hypothesize that differential systemic levels of miRNA expression reflect miRNA dysregulation at sites of spinal inflammation or bone formation where these molecules contribute to the development of pathophysiological features typical of AxSpA.
url http://europepmc.org/articles/PMC5609864?pdf=render
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