Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro
Abstract Background Targeted superparamagnetic iron oxide (SPIO) nanoparticles have emerged as a promising biomarker detection tool for molecular magnetic resonance (MR) image diagnosis. To identify patients who could benefit from Epidermal growth factor receptor (EGFR)-targeted therapies, we introd...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2017-11-01
|
Series: | Journal of Nanobiotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12951-017-0313-2 |
id |
doaj-4a7632566a1249828ac0321b2c3db474 |
---|---|
record_format |
Article |
spelling |
doaj-4a7632566a1249828ac0321b2c3db4742020-11-24T21:12:48ZengBMCJournal of Nanobiotechnology1477-31552017-11-0115111310.1186/s12951-017-0313-2Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitroHuai-Lu Chen0Fei-Ting Hsu1Yu-Chieh Jill Kao2Hua-Shan Liu3Wan-Zhen Huang4Chia-Feng Lu5Ping-Huei Tsai6Ahmed Atef Ahmed Ali7Gilbert Aaron Lee8Ray-Jade Chen9Cheng-Yu Chen10Translational Imaging Research Center, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityDepartment of Medical Research, Taipei Medical University Hospital, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityDepartment of Surgery, School of Medicine, College of Medicine, Taipei Medical UniversityTranslational Imaging Research Center, College of Medicine, Taipei Medical UniversityAbstract Background Targeted superparamagnetic iron oxide (SPIO) nanoparticles have emerged as a promising biomarker detection tool for molecular magnetic resonance (MR) image diagnosis. To identify patients who could benefit from Epidermal growth factor receptor (EGFR)-targeted therapies, we introduce lipid-encapsulated SPIO nanoparticles and hypothesized that anti-EGFR antibody cetuximab conjugated of such nanoparticles can be used to identify EGFR-positive glioblastomas in non-invasive T2 MR image assays. The newly introduced lipid-coated SPIOs, which imitate biological cell surface and thus inherited innate nonfouling property, were utilized to reduce nonspecific binding to off-targeted cells and prevent agglomeration that commonly occurs in nanoparticles. Results The synthesized targeted EGFR-antibody-conjugated SPIO (EGFR-SPIO) nanoparticles were characterized using dynamic light scattering, zeta potential assays, gel electrophoresis mobility shift assays, transmission electron microscopy (TEM) images, and cell line affinity assays, and the results showed that the conjugation was successful. The targeting efficiency of the synthesized EGFR-SPIO nanoparticles was confirmed through Prussian blue staining and TEM images by using glioblastoma cell lines with high or low EGFR expression levels. The EGFR-SPIO nanoparticles preferentially targeted U-251 cells, which have high EGFR expression, and were internalized by cells in a prolonged incubation condition. Moreover, the T2 MR relaxation time of EGFR-SPIO nanoparticles could be used for successfully identifying glioblastoma cells with elevated EGFR expression in vitro and distinguishing U-251 cells from U-87MG cells, which have low EFGR expression. Conclusion These findings reveal that the lipid-encapsulated EGFR-SPIO nanoparticles can specifically target cells with elevated EGFR expression in the three tested human glioblastoma cell lines. The results of this study can be used for noninvasive molecular MR image diagnosis in the future.http://link.springer.com/article/10.1186/s12951-017-0313-2Epidermal growth factor receptor (EGFR)Targeted superparamagnetic iron oxide (SPIO) nanoparticleMagnetic resonance imaging (MRI)GlioblastomaLipid-encapsulated nanoparticle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huai-Lu Chen Fei-Ting Hsu Yu-Chieh Jill Kao Hua-Shan Liu Wan-Zhen Huang Chia-Feng Lu Ping-Huei Tsai Ahmed Atef Ahmed Ali Gilbert Aaron Lee Ray-Jade Chen Cheng-Yu Chen |
spellingShingle |
Huai-Lu Chen Fei-Ting Hsu Yu-Chieh Jill Kao Hua-Shan Liu Wan-Zhen Huang Chia-Feng Lu Ping-Huei Tsai Ahmed Atef Ahmed Ali Gilbert Aaron Lee Ray-Jade Chen Cheng-Yu Chen Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro Journal of Nanobiotechnology Epidermal growth factor receptor (EGFR) Targeted superparamagnetic iron oxide (SPIO) nanoparticle Magnetic resonance imaging (MRI) Glioblastoma Lipid-encapsulated nanoparticle |
author_facet |
Huai-Lu Chen Fei-Ting Hsu Yu-Chieh Jill Kao Hua-Shan Liu Wan-Zhen Huang Chia-Feng Lu Ping-Huei Tsai Ahmed Atef Ahmed Ali Gilbert Aaron Lee Ray-Jade Chen Cheng-Yu Chen |
author_sort |
Huai-Lu Chen |
title |
Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro |
title_short |
Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro |
title_full |
Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro |
title_fullStr |
Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro |
title_full_unstemmed |
Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro |
title_sort |
identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro |
publisher |
BMC |
series |
Journal of Nanobiotechnology |
issn |
1477-3155 |
publishDate |
2017-11-01 |
description |
Abstract Background Targeted superparamagnetic iron oxide (SPIO) nanoparticles have emerged as a promising biomarker detection tool for molecular magnetic resonance (MR) image diagnosis. To identify patients who could benefit from Epidermal growth factor receptor (EGFR)-targeted therapies, we introduce lipid-encapsulated SPIO nanoparticles and hypothesized that anti-EGFR antibody cetuximab conjugated of such nanoparticles can be used to identify EGFR-positive glioblastomas in non-invasive T2 MR image assays. The newly introduced lipid-coated SPIOs, which imitate biological cell surface and thus inherited innate nonfouling property, were utilized to reduce nonspecific binding to off-targeted cells and prevent agglomeration that commonly occurs in nanoparticles. Results The synthesized targeted EGFR-antibody-conjugated SPIO (EGFR-SPIO) nanoparticles were characterized using dynamic light scattering, zeta potential assays, gel electrophoresis mobility shift assays, transmission electron microscopy (TEM) images, and cell line affinity assays, and the results showed that the conjugation was successful. The targeting efficiency of the synthesized EGFR-SPIO nanoparticles was confirmed through Prussian blue staining and TEM images by using glioblastoma cell lines with high or low EGFR expression levels. The EGFR-SPIO nanoparticles preferentially targeted U-251 cells, which have high EGFR expression, and were internalized by cells in a prolonged incubation condition. Moreover, the T2 MR relaxation time of EGFR-SPIO nanoparticles could be used for successfully identifying glioblastoma cells with elevated EGFR expression in vitro and distinguishing U-251 cells from U-87MG cells, which have low EFGR expression. Conclusion These findings reveal that the lipid-encapsulated EGFR-SPIO nanoparticles can specifically target cells with elevated EGFR expression in the three tested human glioblastoma cell lines. The results of this study can be used for noninvasive molecular MR image diagnosis in the future. |
topic |
Epidermal growth factor receptor (EGFR) Targeted superparamagnetic iron oxide (SPIO) nanoparticle Magnetic resonance imaging (MRI) Glioblastoma Lipid-encapsulated nanoparticle |
url |
http://link.springer.com/article/10.1186/s12951-017-0313-2 |
work_keys_str_mv |
AT huailuchen identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT feitinghsu identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT yuchiehjillkao identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT huashanliu identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT wanzhenhuang identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT chiafenglu identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT pinghueitsai identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT ahmedatefahmedali identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT gilbertaaronlee identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT rayjadechen identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro AT chengyuchen identificationofepidermalgrowthfactorreceptorpositiveglioblastomausinglipidencapsulatedtargetedsuperparamagneticironoxidenanoparticlesinvitro |
_version_ |
1716749848522260480 |