Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery
In this study, fluorescein-labelled wheat germ agglutinin (fWGA)-conjugated disulfide cross-linked sodium alginate nanoparticles were developed to specifically target docetaxel (DTX) to colon cancer cells. Different amounts of 3-mercaptopropionic acid (MPA) were covalently attached to sodium alginat...
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doaj-4a8055d8b5ff4a98ae518c47c23e19062020-11-25T01:38:06ZengMDPI AGPharmaceutics1999-49232020-01-011213810.3390/pharmaceutics12010038pharmaceutics12010038Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug DeliveryHock Ing Chiu0Asila Dinie Ayub1Siti Nur Aishah Mat Yusuf2Noorfatimah Yahaya3Erazuliana Abd Kadir4Vuanghao Lim5Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, MalaysiaIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, MalaysiaIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, MalaysiaIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, MalaysiaIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, MalaysiaIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, MalaysiaIn this study, fluorescein-labelled wheat germ agglutinin (fWGA)-conjugated disulfide cross-linked sodium alginate nanoparticles were developed to specifically target docetaxel (DTX) to colon cancer cells. Different amounts of 3-mercaptopropionic acid (MPA) were covalently attached to sodium alginate to form thiolated sodium alginate (MPA1−5). These polymers were then self-assembled and air-oxidised to form disulfide cross-linked nanoparticles (MP1−5) under sonication. DTX was successfully loaded into the resulting MP1−5 to form DTX-loaded nanoparticles (DMP1−5). DMP2 had the highest loading efficiency (17.8%), thus was chosen for fWGA surface conjugation to form fWGA-conjugated nanoparticles (fDMP2) with a conjugation efficiency of 14.1%. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses showed spherical nanoparticles, and an in vitro drug release study recorded a cumulative drug release of 48.6%. Dynamic light scattering (DLS) analysis revealed a mean diameter (MD) of 289 nm with a polydispersity index (PDI) of 0.3 and a zeta potential of −2.2 mV for fDMP2. HT-29 human colon cancer cells treated with fDMP2 showed lower viability than that of L929 mouse fibroblast cells. These results indicate that fDMP2 was efficiently taken up by HT-29 cells (29.9%). Fluorescence and confocal imaging analyses also showed possible internalisation of nanoparticles by HT-29 cells. In conclusion, fDMP2 shows promise as a DTX carrier for colon cancer drug delivery.https://www.mdpi.com/1999-4923/12/1/38disulfide cross-linked nanoparticlesthiolated sodium alginatewheat germ agglutinin conjugationht-29docetaxel |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hock Ing Chiu Asila Dinie Ayub Siti Nur Aishah Mat Yusuf Noorfatimah Yahaya Erazuliana Abd Kadir Vuanghao Lim |
spellingShingle |
Hock Ing Chiu Asila Dinie Ayub Siti Nur Aishah Mat Yusuf Noorfatimah Yahaya Erazuliana Abd Kadir Vuanghao Lim Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery Pharmaceutics disulfide cross-linked nanoparticles thiolated sodium alginate wheat germ agglutinin conjugation ht-29 docetaxel |
author_facet |
Hock Ing Chiu Asila Dinie Ayub Siti Nur Aishah Mat Yusuf Noorfatimah Yahaya Erazuliana Abd Kadir Vuanghao Lim |
author_sort |
Hock Ing Chiu |
title |
Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery |
title_short |
Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery |
title_full |
Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery |
title_fullStr |
Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery |
title_full_unstemmed |
Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery |
title_sort |
docetaxel-loaded disulfide cross-linked nanoparticles derived from thiolated sodium alginate for colon cancer drug delivery |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2020-01-01 |
description |
In this study, fluorescein-labelled wheat germ agglutinin (fWGA)-conjugated disulfide cross-linked sodium alginate nanoparticles were developed to specifically target docetaxel (DTX) to colon cancer cells. Different amounts of 3-mercaptopropionic acid (MPA) were covalently attached to sodium alginate to form thiolated sodium alginate (MPA1−5). These polymers were then self-assembled and air-oxidised to form disulfide cross-linked nanoparticles (MP1−5) under sonication. DTX was successfully loaded into the resulting MP1−5 to form DTX-loaded nanoparticles (DMP1−5). DMP2 had the highest loading efficiency (17.8%), thus was chosen for fWGA surface conjugation to form fWGA-conjugated nanoparticles (fDMP2) with a conjugation efficiency of 14.1%. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses showed spherical nanoparticles, and an in vitro drug release study recorded a cumulative drug release of 48.6%. Dynamic light scattering (DLS) analysis revealed a mean diameter (MD) of 289 nm with a polydispersity index (PDI) of 0.3 and a zeta potential of −2.2 mV for fDMP2. HT-29 human colon cancer cells treated with fDMP2 showed lower viability than that of L929 mouse fibroblast cells. These results indicate that fDMP2 was efficiently taken up by HT-29 cells (29.9%). Fluorescence and confocal imaging analyses also showed possible internalisation of nanoparticles by HT-29 cells. In conclusion, fDMP2 shows promise as a DTX carrier for colon cancer drug delivery. |
topic |
disulfide cross-linked nanoparticles thiolated sodium alginate wheat germ agglutinin conjugation ht-29 docetaxel |
url |
https://www.mdpi.com/1999-4923/12/1/38 |
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