A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These compounds may perform direct defensive functions, i.e., acting as antioxidant, antibacterial, or antifungal agents, or indirectly by signaling the act...
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2017-07-01
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doaj-4a807f75e7fe4b5fba85c238cf74b5342020-11-25T00:10:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01188278706A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringaeMaría Pilar López-GresaPurificación LisónLaura CamposIsmael RodrigoJosé Luis RamblaAntonio GranellVicente ConejeroJosé María BellésVolatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These compounds may perform direct defensive functions, i.e., acting as antioxidant, antibacterial, or antifungal agents, or indirectly by signaling the activation of the plant’s defensive responses. Using a non-targeted GC-MS metabolomics approach, we identified the profile of the VOCs associated with the differential immune response of the Rio Grande tomato leaves infected with either virulent or avirulent strains of Pseudomonas syringae DC3000 pv. tomato. The VOC profile of the tomato leaves infected with avirulent bacteria is characterized by esters of (Z)-3-hexenol with acetic, propionic, isobutyric or butyric acids, and several hydroxylated monoterpenes, e.g., linalool, α-terpineol, and 4-terpineol, which defines the profile of an immunized plant response. In contrast, the same tomato cultivar infected with the virulent bacteria strain produced a VOC profile characterized by monoterpenes and SA derivatives. Interestingly, the differential VOCs emission correlated statistically with the induction of the genes involved in their biosynthetic pathway. Our results extend plant defense system knowledge and suggest the possibility for generating plants engineered to over-produce these VOCs as a complementary strategy for resistance.http://journal.frontiersin.org/article/10.3389/fpls.2017.01188/fullmetabolomicstomatobacteriaVOCsdefense |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Pilar López-Gresa Purificación Lisón Laura Campos Ismael Rodrigo José Luis Rambla Antonio Granell Vicente Conejero José María Bellés |
spellingShingle |
María Pilar López-Gresa Purificación Lisón Laura Campos Ismael Rodrigo José Luis Rambla Antonio Granell Vicente Conejero José María Bellés A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae Frontiers in Plant Science metabolomics tomato bacteria VOCs defense |
author_facet |
María Pilar López-Gresa Purificación Lisón Laura Campos Ismael Rodrigo José Luis Rambla Antonio Granell Vicente Conejero José María Bellés |
author_sort |
María Pilar López-Gresa |
title |
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae |
title_short |
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae |
title_full |
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae |
title_fullStr |
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae |
title_full_unstemmed |
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae |
title_sort |
non-targeted metabolomics approach unravels the vocs associated with the tomato immune response against pseudomonas syringae |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-07-01 |
description |
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These compounds may perform direct defensive functions, i.e., acting as antioxidant, antibacterial, or antifungal agents, or indirectly by signaling the activation of the plant’s defensive responses. Using a non-targeted GC-MS metabolomics approach, we identified the profile of the VOCs associated with the differential immune response of the Rio Grande tomato leaves infected with either virulent or avirulent strains of Pseudomonas syringae DC3000 pv. tomato. The VOC profile of the tomato leaves infected with avirulent bacteria is characterized by esters of (Z)-3-hexenol with acetic, propionic, isobutyric or butyric acids, and several hydroxylated monoterpenes, e.g., linalool, α-terpineol, and 4-terpineol, which defines the profile of an immunized plant response. In contrast, the same tomato cultivar infected with the virulent bacteria strain produced a VOC profile characterized by monoterpenes and SA derivatives. Interestingly, the differential VOCs emission correlated statistically with the induction of the genes involved in their biosynthetic pathway. Our results extend plant defense system knowledge and suggest the possibility for generating plants engineered to over-produce these VOCs as a complementary strategy for resistance. |
topic |
metabolomics tomato bacteria VOCs defense |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01188/full |
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