Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells
Zhongyan Tang,1,* Lihua Lu,2,* Zhengxiang Xia3 1Department of Emergency and Critical Care Medicine, Jin Shan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2Department of Neonatology, International Peace Maternity and Child Health Hospital, School of Medicine, Sha...
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doaj-4a88dc6673d742688569049307a6435c2020-11-25T01:38:36ZengDove Medical PressDrug Design, Development and Therapy1177-88812020-09-01Volume 143965397657408Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers CellsTang ZLu LXia ZZhongyan Tang,1,* Lihua Lu,2,* Zhengxiang Xia3 1Department of Emergency and Critical Care Medicine, Jin Shan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2Department of Neonatology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, People’s Republic of China; 3Department of Pharmacy, School & Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, Shanghai 200072, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhengxiang Xia Tel +8621-66315500Email xzx5380537@163.comBackground: Ovarian cancer (OC) is a serious public health concern in the world. It is important to develop novel drugs to inhibit OC.Purpose: This study investigated the isolation, elucidation, efficiency, molecular docking, and pharmaceutical mechanisms of xanthones isolated from Garcinia nujiangensis.Methods: Xanthones were isolated, and purified by different chromatography, including silica gel, reversed-phase silica gel (ODS-C18), and semipreparative HPLC, then identified by analysis of their spectral data. Three xanthones were estimated for their efficiency on the human OC cells HEY and ES-2. 2 was found to be the most potent cytotoxic xanthones of those tested. Further, its mechanisms of action were explored by molecular docking, cell apoptosis, and Western blotting analysis.Results: Bioassay-guided fractionation of the fruits of Garcinia nujiangensis led to the separation of a new xanthone named nujiangexanthone G ( 1) and two known xanthones. Among these, isojacareubin ( 2) exhibited the most potent cytotoxic compound against the HEY and ES-2 cell lines. The analysis of Western blot suggested that 2 inhibited OC via regulating the PARP, PI3K/AKT/mTOR, and ERK/MAPK signal pathways in the HEY cell lines.Conclusion: In conclusion, isojacareubin ( 2) might be a potential drug for the treatment of OC.Keywords: Garcinia nujiangensis, xanthone, ovarian cancer; OC, molecular docking, apoptosishttps://www.dovepress.com/anti-tumor-xanthones-from-garcinia-nujiangensis-suppress-proliferation-peer-reviewed-article-DDDTgarcinia nujiangensisxanthoneovarian cancermolecular dockingapoptosis |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Tang Z Lu L Xia Z |
spellingShingle |
Tang Z Lu L Xia Z Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells Drug Design, Development and Therapy garcinia nujiangensis xanthone ovarian cancer molecular docking apoptosis |
author_facet |
Tang Z Lu L Xia Z |
author_sort |
Tang Z |
title |
Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells |
title_short |
Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells |
title_full |
Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells |
title_fullStr |
Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells |
title_full_unstemmed |
Anti-Tumor Xanthones from Garcinia nujiangensis Suppress Proliferation, and Induce Apoptosis via PARP, PI3K/AKT/mTOR, and MAPK/ERK Signaling Pathways in Human Ovarian Cancers Cells |
title_sort |
anti-tumor xanthones from garcinia nujiangensis suppress proliferation, and induce apoptosis via parp, pi3k/akt/mtor, and mapk/erk signaling pathways in human ovarian cancers cells |
publisher |
Dove Medical Press |
series |
Drug Design, Development and Therapy |
issn |
1177-8881 |
publishDate |
2020-09-01 |
description |
Zhongyan Tang,1,* Lihua Lu,2,* Zhengxiang Xia3 1Department of Emergency and Critical Care Medicine, Jin Shan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2Department of Neonatology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, People’s Republic of China; 3Department of Pharmacy, School & Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, Shanghai 200072, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhengxiang Xia Tel +8621-66315500Email xzx5380537@163.comBackground: Ovarian cancer (OC) is a serious public health concern in the world. It is important to develop novel drugs to inhibit OC.Purpose: This study investigated the isolation, elucidation, efficiency, molecular docking, and pharmaceutical mechanisms of xanthones isolated from Garcinia nujiangensis.Methods: Xanthones were isolated, and purified by different chromatography, including silica gel, reversed-phase silica gel (ODS-C18), and semipreparative HPLC, then identified by analysis of their spectral data. Three xanthones were estimated for their efficiency on the human OC cells HEY and ES-2. 2 was found to be the most potent cytotoxic xanthones of those tested. Further, its mechanisms of action were explored by molecular docking, cell apoptosis, and Western blotting analysis.Results: Bioassay-guided fractionation of the fruits of Garcinia nujiangensis led to the separation of a new xanthone named nujiangexanthone G ( 1) and two known xanthones. Among these, isojacareubin ( 2) exhibited the most potent cytotoxic compound against the HEY and ES-2 cell lines. The analysis of Western blot suggested that 2 inhibited OC via regulating the PARP, PI3K/AKT/mTOR, and ERK/MAPK signal pathways in the HEY cell lines.Conclusion: In conclusion, isojacareubin ( 2) might be a potential drug for the treatment of OC.Keywords: Garcinia nujiangensis, xanthone, ovarian cancer; OC, molecular docking, apoptosis |
topic |
garcinia nujiangensis xanthone ovarian cancer molecular docking apoptosis |
url |
https://www.dovepress.com/anti-tumor-xanthones-from-garcinia-nujiangensis-suppress-proliferation-peer-reviewed-article-DDDT |
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