Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
Chinese cabbage, belonging to Brassica rapa species, is an important vegetable in Eastern Asia. It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low. The lack of an efficient and stable genetic transformation system for C...
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doaj-4a90699695da4f4cb02c38a122dba2622021-03-13T04:24:49ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412021-03-0172117128Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis)Xiaonan Li0Haiyan Li1Yuzhu Zhao2Peixuan Zong3Zongxiang Zhan4Zhongyun Piao5Department of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaCorresponding author: Tel.: +86 24 88487143.; Department of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, ChinaChinese cabbage, belonging to Brassica rapa species, is an important vegetable in Eastern Asia. It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low. The lack of an efficient and stable genetic transformation system for Chinese cabbage has largely limited related gene functional studies. In this study, we firstly developed a regeneration system for Chinese cabbage by optimizing numerous factors, with 93.50% regeneration rate on average. Based on this, a simple and efficient Agrobacterium-mediated genetic transformation method was established, without pre-culture procedure and concentration adjustment of hormone and AgNO3 in co-cultivation and selection media. Using this system, transformants could be obtained within 3.5–4.0 months. Average transformation frequency is up to 10.83%. The establishment of this simple and efficient genetic transformation method paved the way for further gene editing and functional studies in Chinese cabbage.http://www.sciencedirect.com/science/article/pii/S2468014121000170Chinese cabbageAgrobacterium tumefaciensgenetic transformation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaonan Li Haiyan Li Yuzhu Zhao Peixuan Zong Zongxiang Zhan Zhongyun Piao |
spellingShingle |
Xiaonan Li Haiyan Li Yuzhu Zhao Peixuan Zong Zongxiang Zhan Zhongyun Piao Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis) Horticultural Plant Journal Chinese cabbage Agrobacterium tumefaciens genetic transformation |
author_facet |
Xiaonan Li Haiyan Li Yuzhu Zhao Peixuan Zong Zongxiang Zhan Zhongyun Piao |
author_sort |
Xiaonan Li |
title |
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis) |
title_short |
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis) |
title_full |
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis) |
title_fullStr |
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis) |
title_full_unstemmed |
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis) |
title_sort |
establishment of a simple and efficient agrobacterium-mediated genetic transformation system to chinese cabbage (brassica rapa l. ssp. pekinensis) |
publisher |
KeAi Communications Co., Ltd. |
series |
Horticultural Plant Journal |
issn |
2468-0141 |
publishDate |
2021-03-01 |
description |
Chinese cabbage, belonging to Brassica rapa species, is an important vegetable in Eastern Asia. It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low. The lack of an efficient and stable genetic transformation system for Chinese cabbage has largely limited related gene functional studies. In this study, we firstly developed a regeneration system for Chinese cabbage by optimizing numerous factors, with 93.50% regeneration rate on average. Based on this, a simple and efficient Agrobacterium-mediated genetic transformation method was established, without pre-culture procedure and concentration adjustment of hormone and AgNO3 in co-cultivation and selection media. Using this system, transformants could be obtained within 3.5–4.0 months. Average transformation frequency is up to 10.83%. The establishment of this simple and efficient genetic transformation method paved the way for further gene editing and functional studies in Chinese cabbage. |
topic |
Chinese cabbage Agrobacterium tumefaciens genetic transformation |
url |
http://www.sciencedirect.com/science/article/pii/S2468014121000170 |
work_keys_str_mv |
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