| Summary: | Yang Lü,1 Haiquan Kang,2 Jianming Fan3 1State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People’s Republic of China; 2Department of Laboratory Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People’s Republic of China; 3The Laboratory of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People’s Republic of ChinaCorrespondence: Jianming Fan Tel +86-13783617236Email fan5746067@126.comBackground: An ESBL, carbapenemase- and MCR-1-producing Escherichia coli ST648 strain was isolated from the urine sample of a patient in a Chinese tertiary hospital in 2016.Methods: The strain was fully sequenced by GridION X5 platform of Oxford Nanopore Technology.Results: The sequence analysis showed that the extended-spectrum β-lactamases CTX-M-65 and OXA-1, the carbapenemase NDM-5, the MCR-1 were encoded, respectively, by three different resistance plasmids. The pE648CTX-M-65-carrying blaCTX-M-65 was a novel conjugative plasmid belonging to IncHI2 type; except for the blaCTX-M-65, it also carried resistance genes ble, floR, sul1, aph(4)-Ia, aac(3)-VI, aac(6ʹ)-II, blaOXA-1, catB, arr3 and tetA. Besides, an IncX4 plasmid pE648MCR-1-carrying mcr-1 and an IncX3 plasmid pE648NDM-5-carrying blaNDM-5 were also identified.Conclusion: The three transferable resistance plasmids coexisting in the E. coli ST648 isolate indicated the high risk to disseminate the extensively-drug-resistance among Enterobacteriaceae.Keywords: CTX-M-65, MCR-1, NDM-5, extensively-drug-resistance, Escherichia coli
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