Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes
Phytochemicals have gained an immense interest in obesity management. Previously, we have shown that theaflavin-3,3′-digallate (TF3), a black tea polyphenol, prevents adipocyte-triggered metaflammation. Here, we demonstrate that TF3 attenuates triacylglycerol accumulation in adipocytes, concomitant...
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2015-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615002716 |
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doaj-4b056b1dd1d54723a699f856afff48c32021-04-29T04:44:40ZengElsevierJournal of Functional Foods1756-46462015-08-0117271282Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytesHuey-Jiun Ko0Chih-Yu Lo1Be-Jen Wang2Robin Yih-Yuan Chiou3Shu-Mei Lin4Department of Food Science, College of Life Science, National Chiayi University, Chiayi City, TaiwanDepartment of Food Science, College of Life Science, National Chiayi University, Chiayi City, TaiwanDepartment of Food Science, College of Life Science, National Chiayi University, Chiayi City, TaiwanDepartment of Food Science, College of Life Science, National Chiayi University, Chiayi City, TaiwanCorresponding author. Department of Food Science, College of Life Science, National Chiayi University, No. 300 Syuefu Rd., Chiayi City 60004, Taiwan. Tel.: +886 5 271 7625; Fax: +886 5 271 7596.; Department of Food Science, College of Life Science, National Chiayi University, Chiayi City, TaiwanPhytochemicals have gained an immense interest in obesity management. Previously, we have shown that theaflavin-3,3′-digallate (TF3), a black tea polyphenol, prevents adipocyte-triggered metaflammation. Here, we demonstrate that TF3 attenuates triacylglycerol accumulation in adipocytes, concomitant with promoting gene expression profile that favors lipolysis and β-oxidation, and inducing energy dissipation-related genes, mitochondrial uncoupling protein-1 (UCP-1) and UCP-2. The gene expression is in line with the upregulation of peroxisome proliferator-activated receptor α (PPARα), a primary transactivator for the expression of lipolytic genes. TF3 activates AMP-activated protein kinase (AMPK), which is required for TF3 effects of PPARα upregulation, and the reversal of the inactivation of Forkhead-box-O 3A (FoxO3A) and insulin-induced suppression of manganese superoxide dismutase (MnSOD). The role of MnSOD in adipogenesis is verified in MnSOD-overexpressing adipocytes. Thus, our results demonstrate TF3 as a potent AMPK activator with anti-adiposity activity in adipocytes, suggesting its potential application in functional foods and nutraceuticals for obesity management.http://www.sciencedirect.com/science/article/pii/S1756464615002716AdipocyteAMPKBlack teaFoxO3AMnSODTheaflavin-3,3′-digallate |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Huey-Jiun Ko Chih-Yu Lo Be-Jen Wang Robin Yih-Yuan Chiou Shu-Mei Lin |
| spellingShingle |
Huey-Jiun Ko Chih-Yu Lo Be-Jen Wang Robin Yih-Yuan Chiou Shu-Mei Lin Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes Journal of Functional Foods Adipocyte AMPK Black tea FoxO3A MnSOD Theaflavin-3,3′-digallate |
| author_facet |
Huey-Jiun Ko Chih-Yu Lo Be-Jen Wang Robin Yih-Yuan Chiou Shu-Mei Lin |
| author_sort |
Huey-Jiun Ko |
| title |
Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes |
| title_short |
Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes |
| title_full |
Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes |
| title_fullStr |
Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes |
| title_full_unstemmed |
Theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes |
| title_sort |
theaflavin-3,3′-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and ampk–foxo3a–mnsod pathway in 3t3-l1 adipocytes |
| publisher |
Elsevier |
| series |
Journal of Functional Foods |
| issn |
1756-4646 |
| publishDate |
2015-08-01 |
| description |
Phytochemicals have gained an immense interest in obesity management. Previously, we have shown that theaflavin-3,3′-digallate (TF3), a black tea polyphenol, prevents adipocyte-triggered metaflammation. Here, we demonstrate that TF3 attenuates triacylglycerol accumulation in adipocytes, concomitant with promoting gene expression profile that favors lipolysis and β-oxidation, and inducing energy dissipation-related genes, mitochondrial uncoupling protein-1 (UCP-1) and UCP-2. The gene expression is in line with the upregulation of peroxisome proliferator-activated receptor α (PPARα), a primary transactivator for the expression of lipolytic genes. TF3 activates AMP-activated protein kinase (AMPK), which is required for TF3 effects of PPARα upregulation, and the reversal of the inactivation of Forkhead-box-O 3A (FoxO3A) and insulin-induced suppression of manganese superoxide dismutase (MnSOD). The role of MnSOD in adipogenesis is verified in MnSOD-overexpressing adipocytes. Thus, our results demonstrate TF3 as a potent AMPK activator with anti-adiposity activity in adipocytes, suggesting its potential application in functional foods and nutraceuticals for obesity management. |
| topic |
Adipocyte AMPK Black tea FoxO3A MnSOD Theaflavin-3,3′-digallate |
| url |
http://www.sciencedirect.com/science/article/pii/S1756464615002716 |
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