Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling
Chromatin remodeling processes are among the most important regulatory mechanisms in controlling cell proliferation and regeneration. Drosophila intestinal stem cells (ISCs) exhibit self-renewal potentials, maintain tissue homeostasis, and serve as an excellent model for studying cell growth and reg...
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doaj-4b24171330764af496b936c744e898f52021-05-04T22:33:06ZengeLife Sciences Publications LtdeLife2050-084X2013-10-01210.7554/eLife.00999Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signalingYunyun Jin0Jinjin Xu1Meng-Xin Yin2Yi Lu3Lianxin Hu4Peixue Li5Peng Zhang6Zengqiang Yuan7Margaret S Ho8Hongbin Ji9Yun Zhao10Lei Zhang11State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, ChinaDepartment of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaChromatin remodeling processes are among the most important regulatory mechanisms in controlling cell proliferation and regeneration. Drosophila intestinal stem cells (ISCs) exhibit self-renewal potentials, maintain tissue homeostasis, and serve as an excellent model for studying cell growth and regeneration. In this study, we show that Brahma (Brm) chromatin-remodeling complex is required for ISC proliferation and damage-induced midgut regeneration in a lineage-specific manner. ISCs and enteroblasts exhibit high levels of Brm proteins; and without Brm, ISC proliferation and differentiation are impaired. Importantly, the Brm complex participates in ISC proliferation induced by the Scalloped–Yorkie transcriptional complex and that the Hippo (Hpo) signaling pathway directly restricted ISC proliferation by regulating Brm protein levels by inducing caspase-dependent cleavage of Brm. The cleavage resistant form of Brm protein promoted ISC proliferation. Our findings highlighted the importance of Hpo signaling in regulating epigenetic components such as Brm to control downstream transcription and hence ISC proliferation.https://elifesciences.org/articles/00999Hippo signalingbrahmamidgut |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yunyun Jin Jinjin Xu Meng-Xin Yin Yi Lu Lianxin Hu Peixue Li Peng Zhang Zengqiang Yuan Margaret S Ho Hongbin Ji Yun Zhao Lei Zhang |
spellingShingle |
Yunyun Jin Jinjin Xu Meng-Xin Yin Yi Lu Lianxin Hu Peixue Li Peng Zhang Zengqiang Yuan Margaret S Ho Hongbin Ji Yun Zhao Lei Zhang Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling eLife Hippo signaling brahma midgut |
author_facet |
Yunyun Jin Jinjin Xu Meng-Xin Yin Yi Lu Lianxin Hu Peixue Li Peng Zhang Zengqiang Yuan Margaret S Ho Hongbin Ji Yun Zhao Lei Zhang |
author_sort |
Yunyun Jin |
title |
Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling |
title_short |
Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling |
title_full |
Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling |
title_fullStr |
Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling |
title_full_unstemmed |
Brahma is essential for Drosophila intestinal stem cell proliferation and regulated by Hippo signaling |
title_sort |
brahma is essential for drosophila intestinal stem cell proliferation and regulated by hippo signaling |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2013-10-01 |
description |
Chromatin remodeling processes are among the most important regulatory mechanisms in controlling cell proliferation and regeneration. Drosophila intestinal stem cells (ISCs) exhibit self-renewal potentials, maintain tissue homeostasis, and serve as an excellent model for studying cell growth and regeneration. In this study, we show that Brahma (Brm) chromatin-remodeling complex is required for ISC proliferation and damage-induced midgut regeneration in a lineage-specific manner. ISCs and enteroblasts exhibit high levels of Brm proteins; and without Brm, ISC proliferation and differentiation are impaired. Importantly, the Brm complex participates in ISC proliferation induced by the Scalloped–Yorkie transcriptional complex and that the Hippo (Hpo) signaling pathway directly restricted ISC proliferation by regulating Brm protein levels by inducing caspase-dependent cleavage of Brm. The cleavage resistant form of Brm protein promoted ISC proliferation. Our findings highlighted the importance of Hpo signaling in regulating epigenetic components such as Brm to control downstream transcription and hence ISC proliferation. |
topic |
Hippo signaling brahma midgut |
url |
https://elifesciences.org/articles/00999 |
work_keys_str_mv |
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