Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
The inhibition effect of the selected heavy metals (Ag<sup>+</sup>, Cd<sup>2+</sup>, Cu<sup>2+</sup>, and Hg<sup>2+</sup>) on glucose oxidase (GOx) enzyme from <i>Aspergillus niger</i> (EC 1.1.3.4.) was studied using a new amperometric bios...
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doaj-4b2991049cc24753b5180f3d597b09812020-11-25T02:45:32ZengMDPI AGSensors1424-82202019-07-011913293910.3390/s19132939s19132939Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy MetalsAmir M. Ashrafi0Milan Sýs1Eliška Sedláčková2Amir Shaaban Farag3Vojtěch Adam4Jan Přibyl5Lukáš Richtera6Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicCzech Republic CEITEC MU, Nanobiotechnol Group, Kamenice 5, CZ-62 500 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicThe inhibition effect of the selected heavy metals (Ag<sup>+</sup>, Cd<sup>2+</sup>, Cu<sup>2+</sup>, and Hg<sup>2+</sup>) on glucose oxidase (GOx) enzyme from <i>Aspergillus niger</i> (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion<sup>®</sup> layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.https://www.mdpi.com/1424-8220/19/13/2939glucose oxidaseheavy metalsamperometric biosensornon-competitive inhibition |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amir M. Ashrafi Milan Sýs Eliška Sedláčková Amir Shaaban Farag Vojtěch Adam Jan Přibyl Lukáš Richtera |
spellingShingle |
Amir M. Ashrafi Milan Sýs Eliška Sedláčková Amir Shaaban Farag Vojtěch Adam Jan Přibyl Lukáš Richtera Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals Sensors glucose oxidase heavy metals amperometric biosensor non-competitive inhibition |
author_facet |
Amir M. Ashrafi Milan Sýs Eliška Sedláčková Amir Shaaban Farag Vojtěch Adam Jan Přibyl Lukáš Richtera |
author_sort |
Amir M. Ashrafi |
title |
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals |
title_short |
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals |
title_full |
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals |
title_fullStr |
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals |
title_full_unstemmed |
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals |
title_sort |
application of the enzymatic electrochemical biosensors for monitoring non-competitive inhibition of enzyme activity by heavy metals |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2019-07-01 |
description |
The inhibition effect of the selected heavy metals (Ag<sup>+</sup>, Cd<sup>2+</sup>, Cu<sup>2+</sup>, and Hg<sup>2+</sup>) on glucose oxidase (GOx) enzyme from <i>Aspergillus niger</i> (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion<sup>®</sup> layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons. |
topic |
glucose oxidase heavy metals amperometric biosensor non-competitive inhibition |
url |
https://www.mdpi.com/1424-8220/19/13/2939 |
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