TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers
Yaming Du,1,* Wei Zhang,1,* Binghui Du,1 Sheng Zang,1 Xinpeng Wang,1 Xin Mao,1 Zhansheng Hu2 1Department of Vascular Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China; 2Department of Intensive Care Unit, The First Affiliated Hospital of Jinzhou Medical U...
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doaj-4b3abee8d9b4462a926736d7b4b944332020-11-25T01:01:28ZengDove Medical PressOncoTargets and Therapy1178-69302018-11-01Volume 117841785242055TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancersDu YZhang WDu BZang SWang XMao XHu ZYaming Du,1,* Wei Zhang,1,* Binghui Du,1 Sheng Zang,1 Xinpeng Wang,1 Xin Mao,1 Zhansheng Hu2 1Department of Vascular Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China; 2Department of Intensive Care Unit, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China *These authors contributed equally to this work Background: TRIM32 is overexpressed in several human cancers. However, its expression pattern, biological characteristics and mechanisms in human non-small cell lung cancer (NSCLC) have not been reported. Methods: We examined TRIM32 protein in 115 cases of NSCLC specimens. TRIM32 plasmid transfection and siRNA knockdown was carried out in NSCLC cell lines. AnnexinV/PI and JC-1 staining were performed to examine the change of apoptosis and mitochondrial membrane potential. Western blot was used to detect change of downstream proteins. Results: We found that TRIM32 protein was upregulated in 69 cases and positively correlated with advanced TNM stage. TRIM32 overexpression also correlated with poor survival of NSCLC patients. Biological assays demonstrated that TRIM32 overexpression promoted while it depletion inhibited cell growth, colony formation and invasion. In addition, TRIM32 maintained NSCLC cell viability and reduced apoptosis when treated with cisplatin. JC-1 and CellRox staining demonstrated that TRIM32 could maintain mitochondrial membrane potential and reduce Reactive Oxygen Species (ROS) production after cisplatin treatment. Western blot analysis showed that TRIM32 overexpression downregulated caspase 3 cleavage and cytochrome c release. TRIM32 also positively regulated Bcl-2 protein expression and NF-κB signaling. Inhibition of NF-κB abolished the effects of TRIM32 on Bcl-2. Conclusion: Taken together, our results indicated that TRIM32 is overexpressed in NSCLC and regulates cisplatin resistance, possibly through NF-κB and Bcl-2. Keywords: NSCLC, TRIM32, NF-κB, apoptosishttps://www.dovepress.com/trim32-overexpression-improves-chemoresistance-through-regulation-of-m-peer-reviewed-article-OTTNSCLCTRIM32NF-κBapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Du Y Zhang W Du B Zang S Wang X Mao X Hu Z |
spellingShingle |
Du Y Zhang W Du B Zang S Wang X Mao X Hu Z TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers OncoTargets and Therapy NSCLC TRIM32 NF-κB apoptosis |
author_facet |
Du Y Zhang W Du B Zang S Wang X Mao X Hu Z |
author_sort |
Du Y |
title |
TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers |
title_short |
TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers |
title_full |
TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers |
title_fullStr |
TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers |
title_full_unstemmed |
TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers |
title_sort |
trim32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2018-11-01 |
description |
Yaming Du,1,* Wei Zhang,1,* Binghui Du,1 Sheng Zang,1 Xinpeng Wang,1 Xin Mao,1 Zhansheng Hu2 1Department of Vascular Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China; 2Department of Intensive Care Unit, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China *These authors contributed equally to this work Background: TRIM32 is overexpressed in several human cancers. However, its expression pattern, biological characteristics and mechanisms in human non-small cell lung cancer (NSCLC) have not been reported. Methods: We examined TRIM32 protein in 115 cases of NSCLC specimens. TRIM32 plasmid transfection and siRNA knockdown was carried out in NSCLC cell lines. AnnexinV/PI and JC-1 staining were performed to examine the change of apoptosis and mitochondrial membrane potential. Western blot was used to detect change of downstream proteins. Results: We found that TRIM32 protein was upregulated in 69 cases and positively correlated with advanced TNM stage. TRIM32 overexpression also correlated with poor survival of NSCLC patients. Biological assays demonstrated that TRIM32 overexpression promoted while it depletion inhibited cell growth, colony formation and invasion. In addition, TRIM32 maintained NSCLC cell viability and reduced apoptosis when treated with cisplatin. JC-1 and CellRox staining demonstrated that TRIM32 could maintain mitochondrial membrane potential and reduce Reactive Oxygen Species (ROS) production after cisplatin treatment. Western blot analysis showed that TRIM32 overexpression downregulated caspase 3 cleavage and cytochrome c release. TRIM32 also positively regulated Bcl-2 protein expression and NF-κB signaling. Inhibition of NF-κB abolished the effects of TRIM32 on Bcl-2. Conclusion: Taken together, our results indicated that TRIM32 is overexpressed in NSCLC and regulates cisplatin resistance, possibly through NF-κB and Bcl-2. Keywords: NSCLC, TRIM32, NF-κB, apoptosis |
topic |
NSCLC TRIM32 NF-κB apoptosis |
url |
https://www.dovepress.com/trim32-overexpression-improves-chemoresistance-through-regulation-of-m-peer-reviewed-article-OTT |
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