Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells
Hot water and hydroethanolic (70:30) extracts were prepared from 15 plant species, which were investigated to discover eco-friendly and less expensive tick control methods as an alternative to synthetic acaricides. A contact bioassay was used to determine the acaricidal activity of these extracts ag...
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doaj-4b3ca6c0eb6248a89f70c88a7aac35ea2020-11-24T22:05:46ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352019-07-01861e1e710.4102/ojvr.v86i1.1665612Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cellsGerda Fouche0Olubukola T. Adenubi1Tlabo Leboho2Lyndy J. McGaw3Vinny Naidoo4Kevin W. Wellington5Jacobus N. Eloff6Chemistry Department, Faculty of Natural and Agricultural Sciences, University of Pretoria, PretoriaPhytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, OnderstepoortCouncil for Scientific and Industrial Research (CSIR) Biosciences, Pretoriahytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, OnderstepoortBiomedical Research Centre, Faculty Veterinary Science, University of Pretoria, OnderstepoortCouncil for Scientific and Industrial Research (CSIR) Biosciences, PretoriaPhytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, OnderstepoortHot water and hydroethanolic (70:30) extracts were prepared from 15 plant species, which were investigated to discover eco-friendly and less expensive tick control methods as an alternative to synthetic acaricides. A contact bioassay was used to determine the acaricidal activity of these extracts against the cattle tick, Rhipicephalus turanicus (Acari: Ixodidae) at a concentration of 20% (200 mg/mL). The hydroethanolic extracts had better activity than the hot water extracts against R. turanicus. The hydroethanolic extract from Tabernaemontana elegans (leaves) had the best mortality (87.0%). This was followed by Calpurnia aurea (stems) with a mortality of 75.0%, Schkuhria pinnata (whole plant) with a mortality of 67.0% and Aloe rupestris (leaves) with a mortality of 66.6%. The toxicity of the plant extracts was also investigated and it was found that most of the hydroethanolic and hot water extracts were either safe or very safe on human Vero kidney and liver HepG2 cells. From this study, it was evident that botanicals have the potential to be developed as environmentally benign natural acaricides against R. turanicus.https://ojvr.org/index.php/ojvr/article/view/1665Rhipicephalus turanicuscontact bioassayacaricidal activityVero cellsHepG2 cellstoxicitywaterethanol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gerda Fouche Olubukola T. Adenubi Tlabo Leboho Lyndy J. McGaw Vinny Naidoo Kevin W. Wellington Jacobus N. Eloff |
spellingShingle |
Gerda Fouche Olubukola T. Adenubi Tlabo Leboho Lyndy J. McGaw Vinny Naidoo Kevin W. Wellington Jacobus N. Eloff Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells Onderstepoort Journal of Veterinary Research Rhipicephalus turanicus contact bioassay acaricidal activity Vero cells HepG2 cells toxicity water ethanol |
author_facet |
Gerda Fouche Olubukola T. Adenubi Tlabo Leboho Lyndy J. McGaw Vinny Naidoo Kevin W. Wellington Jacobus N. Eloff |
author_sort |
Gerda Fouche |
title |
Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells |
title_short |
Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells |
title_full |
Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells |
title_fullStr |
Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells |
title_full_unstemmed |
Acaricidal activity of the aqueous and hydroethanolic extracts of 15 South African plants against Rhipicephalus turanicus and their toxicity on human liver and kidney cells |
title_sort |
acaricidal activity of the aqueous and hydroethanolic extracts of 15 south african plants against rhipicephalus turanicus and their toxicity on human liver and kidney cells |
publisher |
AOSIS |
series |
Onderstepoort Journal of Veterinary Research |
issn |
0030-2465 2219-0635 |
publishDate |
2019-07-01 |
description |
Hot water and hydroethanolic (70:30) extracts were prepared from 15 plant species, which were investigated to discover eco-friendly and less expensive tick control methods as an alternative to synthetic acaricides. A contact bioassay was used to determine the acaricidal activity of these extracts against the cattle tick, Rhipicephalus turanicus (Acari: Ixodidae) at a concentration of 20% (200 mg/mL). The hydroethanolic extracts had better activity than the hot water extracts against R. turanicus. The hydroethanolic extract from Tabernaemontana elegans (leaves) had the best mortality (87.0%). This was followed by Calpurnia aurea (stems) with a mortality of 75.0%, Schkuhria pinnata (whole plant) with a mortality of 67.0% and Aloe rupestris (leaves) with a mortality of 66.6%. The toxicity of the plant extracts was also investigated and it was found that most of the hydroethanolic and hot water extracts were either safe or very safe on human Vero kidney and liver HepG2 cells. From this study, it was evident that botanicals have the potential to be developed as environmentally benign natural acaricides against R. turanicus. |
topic |
Rhipicephalus turanicus contact bioassay acaricidal activity Vero cells HepG2 cells toxicity water ethanol |
url |
https://ojvr.org/index.php/ojvr/article/view/1665 |
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